powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)

メチルチアゾリルジフェニル-テトラゾリウムブロミド, MTT, 3-(4,5-ジメチル-2-チアゾリル)-2,5-ジフェニル-2H-テトラゾリウムブロミド
Empirical Formula (Hill Notation):
EC 番号:
PubChem Substance ID:






≥97.5% (HPLC)




cell culture | insect: suitable
cell culture | mammalian: suitable


195 °C (dec.) (lit.)


H2O: 5 mg/mL



SMILES string




InChI key


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チアゾリルブルーテトラゾリウムブルー(MTT)を細胞増殖の測定に使用する場合があります。 MTTは黄色の溶液を生じますが、これは生細胞のミトコンドリアデヒドロゲナーゼにより濃青色の水不溶性MTTホルマザンに変換されます。青色の結晶は酸性化イソプロパノールで可溶化され、570 nmで比色的に強度を測定します。MTTは、組織化学的試薬/細胞化学的試薬として、また、NADを検出するために使用されています。 組織中のADP結合酵素系は、使用されるシアニド(シアン化物)トラップによりカチオンが結合するため、MTTでは検出することができません。 MTTは急速にホルマザンに還元され、ホルマザンはニッケル、銅、およびコバルトでキレート化されます;コバルトキレートは酸化系で使用されています。


1 g in glass bottle
15 mg in poly bottle
100, 500 mg in glass insert


Exclamation markHealth hazard





Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3


Respiratory system


11 - Combustible Solids




Not applicable


Not applicable

個人用保護具 (PPE)

Eyeshields, Gloves, type N95 (US)





  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. What can be used to make a solution of Thiazolyl Blue Tetrazolium Bromide (MTT)?

    We test the solubility of MTT in water at a concentration of 5 mg/mL.  For the MTT viability assay, we dissolve Product No. M5655 at a concentration of 5 mg/ml in RPMI-1640 without phenol red. This medium is available as a powder (Product No.R8755) or liquid (Product No. R7509). MTT can be solubilized in any culture media or balanced salt solution that does not contain phenol red as a pH indicator.

  3. How can I store solutions of Thiazolyl Blue Tetrazolium Bromide (MTT)?

    Reconstituted MTT solution is stable for at least 6 months when stored frozen (-20 °C). Storage of reconstituted MTT solution at 2-8°C for more than 2 weeks may cause decomposition and yield erroneous results.

  4. What can be used to solubilize MTT formazan (the product of the Thiazolyl Blue Tetrazolium Bromide - MTT reaction)?

    Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring of the MTT, yielding purple MTT formazan crystals. These crystals are not soluble in aqueous solutions; they may be dissolved in acidified isopropanol (0.1 N HCl in anhydrous isopropanol). If this does not lyse the cells, Triton X-100 can be added to the acidified isopropanol. A typical working concentration is 10% Triton X-100 (i.e., 1 volume of 100% Triton X-100 + 9 volumes of acidified isopropanol).

  5. Why is a reference wavelength used for data analysis of Thiazolyl Blue Tetrazolium Bromide (MTT)?

    The reference wavelength (630-690 nm) is used to adjust for the optical variation in the wells of the plate.  It also corrects for dust and fingerprints that can be present.  The assay is read at 570 nm.  The reference wavelength needs to be far enough away from the assay wavelength so as not to affect those values.

  6. What type of multi-well plate should be used for the Thiazolyl Blue Tetrazolium Bromide (MTT) assay?

    It is recommended that flat bottom multi-well plates be used for the MTT assay. The use of tissue culture treated plates is only important if necessary for good cell growth.  If cells are grown in larger plates, the supernatant after cell lysis can be transferred to a 96 well plate for reading.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. Is the MTT assay quantitative?

    The MTT asay is a method to assess cell viability.  This assay is a semiquantitative assay. The assay is used to compare the viability changes in treated cells to untreated cells. The absorbance is indicative of the cell number. The higher the absorbance, the greater the number of viable cells present. Most researchers compare the absorbance of the two samples as a ratio (ABS treated cells/ABS untreated cells) to get a fold increase/decrease in cell number.   

  11. How can Thiazolyl Blue Tetrazolium Bromide be used for staining dehydrogenase in tissue sections?

    Products M2128 or M5655, Thiazolyl Blue Tetrazolium Bromide can be used for staining dehydrogenase in tissue sections. See the method below. Reagent PreparationMTT (2 mg/ml distilled water) 2.5 ml0.2 M Tris buffer, pH 7.4 2.5 ml0.5 M cobalt chloride 0.5 ml0.05 M magnesium chloride 1.0 mlDistilled water 2.5 mlThe pH of this solution is adjusted to 7.0 if necessary. Aliquot and store at -20°C until required. Sample PreparationCut cytostat sections at 5 to 7 microns. Do not fix. Method:1. Incubate sections in appropriate incubation solution at 37°C for 30-60 minutes.2. Transfer sections to 15% formol saline, 15 minutes.3. Wash in distilled water4. Counterstain in 2% methyl green if required.5. Wash in distilled water.6. Mount in glycerin Jelly. ResultsEnzyme: Black formazan deposits with MTT Source:  Bancroft and Gamble, Theory and Practice of Histological Techniques, Fifth Edition. Churchill Livingstone, London, 2002, page 610.

  12. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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