Merck
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M8787

Sigma-Aldrich

塩化マグネシウム 溶液

PCR Reagent, 25 mM MgCI2 solution for PCR

Synonym(s):
PCR optimization
Linear Formula:
MgCl2
CAS Number:
Molecular Weight:
95.21
MDL番号:
PubChem Substance ID:
NACRES:
NA.52

グレード

PCR Reagent

品質水準

300

形態

liquid

包装

vial of 1.5 mL

濃度

25 mM±1 mM

technique(s)

PCR: suitable

colorless

application(s)

agriculture
diagnostic assay manufacturing

その他の活性

DNase, RNase, none detected

保管温度

2-8°C

SMILES string

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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詳細

Magnesium chloride solution is suitable for the optimization of polymerase chain reactions (PCR). Magnesium chloride acts as a source of magnesium ions for polymerase chain reactions (PCR). It influences the primer-template annealing temperature, fidelity, specificity, and yield. Excess amounts of magnesium can cause an increase in nonspecific products, while lack of magnesium can lead to reduced yield.

アプリケーション

Magnesium chloride solution has been used as a component of the:

  • amplification mixture for polymerase chain reaction (PCR)
  • amplification mixture in an inter simple sequence repeats (ISSR)-PCR
  • reaction buffer in 16S-23S rRNA gene internal transcribed spacer (ITS) PCR amplification and restriction analysis (ITS-PCR-RFLP)
  • PCR master mix for quantitative reverse transcription-polymerase chain reaction (qRT PCR) to amplify reversely transcribed cDNA
  • reaction mix for RT-PCR to assess the gene expression

適合性

ポリメラ-ゼ連鎖反応の最適化用です。

保管分類コード

12 - Non Combustible Liquids

WGK

nwg

引火点(°F)

Not applicable

引火点(℃)

Not applicable

個人用保護具 (PPE)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificate of Analysis

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Certificate of Origin

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