M9070

Sigma-Aldrich

Matrix Metalloproteinase-2 ヒト

>90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

別名:
72 Kd Type IV Collagenase, 72 Kd Gelatinase, MMP-2, Gelatinase A, 72 Kd Gelatinase 72 Kd Type IV collagenase Gelatinase A
MDL番号:
NACRES:
NA.32

品質水準

200

リコンビナント

expressed in NSO cells

アッセイ

>90% (SDS-PAGE)

形態

lyophilized powder

分子量

apparent mol wt ~69 kDa

UniProtアクセッション番号

保管温度

−20°C

Gene Information

human ... MMP2(4313)

詳細

Matrix metalloproteinase-2 (MMP-2) also known as gelatinase or type IV collagenase is a 72kDa protein. MMP-2 is a member of matrix metalloproteinase (MMP) family of enzymes. Basic structure of MMP2 contains signal peptide domain that targets the enzyme for secretion, the pro-peptide domain, which is removed when the enzyme is activated and the catalytic site containing gelatin-binding domain.

特異性

MMP-2 specifically cleaves type IV collagen, a major structural component of basement membranes.

アプリケーション

Matrix metalloproteinase-2 (MMP2) human has been used:
  • As a standard in zymography to measure gelatinolytic activity of MMP2.
  • In enzymatic method for dissociation of brain tissue to single cells.

生物化学的/生理学的作用

Matrix metalloproteinase-2 (MMP-2) cleaves gelatin, type IV, V, VII, X, and XI collagens, fibronectin, elastin, laminin, proteoglycans, and a range of non extracellular matrix (ECM ) components. MMP-2 cleaves native type I collagen to N-terminal ¾ and C-terminal ¼ fragments identical to those generated by interstitial collagenases. MMP2 and MMP9 play an essential role in matrix degradation and they are implicated in the maintenance of neovascularization. MMP-2 activity is associated with human ovarian follicular development. MMP2 expression is elevated in human myocardial infarction and dilated cardiomyopathy. Wound fluid from chronic leg ulcers show elevated expression of MMP2. Overexpression of active MMP2 affects wound healing in chronic leg ulcers. MMP2 activity is inhibited by tissue inhibitor of metalloproteinase-2 (TIMP-2). Human follicular fluid MMP-2 level acts as a potential biomarker of oocyte maturation in in vitro fertilization and intracytoplasmic sperm injection (ICSI) cycles.
MMP-2 degrades general matrix components and may have a role in processes such as host defense, cell proliferation, and protein turnover as well as tissue remodeling.

物理的形状

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 5 mM CaCl2, 150 mM NaCl, and 1 μM ZnCl2, pH 7.5.

再構成

It is recommended that 0.1 mL of buffer (100 mM Tris, 10 mM calcium chloride, 150 mM sodium chloride, and 0.05% Brij® L23, pH 8.0) be used to give a stock solution of the enzyme at 100 μg/mL.

アナリシスノート

The biological activity is measured by its ability to cleave a fluorogenic peptide substrate.

法的情報

Brij is a registered trademark of Croda International PLC

storage_class_code

12 - Non Combustible Liquids

ドイツ水質汚染分類

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

個人用保護具 (PPE)

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

試験成績書(COA)

原産地証明書(COO)

A non-aggressive, highly efficient, enzymatic method for dissociation of human brain-tumors and brain-tissues to viable single-cells.
Volovitz I
BMC Neuroscience, 17(1):30 (2016)
Immunohistochemical expression of MMP-14 and MMP-2, and MMP-2 activity during human ovarian follicular development.
Vos MC
Reproductive Biology and Endocrinology, 12:12 (2014)
Matrix metalloproteinase 2 level in human follicular fluid is a reliable marker of human oocyte maturation in in vitro fertilization and intracytoplasmic sperm injection cycles.
Yang WJ
Reproductive Biology and Endocrinology, 13:102 (2015)
Takashi Temma et al.
PloS one, 9(7), e102180-e102180 (2014-07-11)
Since matrix metalloproteinase-2 (MMP-2) is an important marker of tumor malignancy, we developed an original drug design strategy, MMP-2 activity dependent anchoring probes (MDAP), for use in MMP-2 activity imaging, and evaluated the usefulness of this probe in in vitro...
Matrix metalloproteinases and myocardial infarction
Phatharajaree W
The Canadian Journal of Cardiology, 23(9), 727-733 (2007)

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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