P6874

Sigma-Aldrich

Anti-p53 antibody, Mouse monoclonal

clone DO-1, purified from hybridoma cell culture

別名:
抗TRP53抗体
NACRES:
NA.41

品質水準

200

由来生物

mouse

抗体製品の状態

purified immunoglobulin

antibody product type

primary antibodies

クローン

DO-1, monoclonal

形態

buffered aqueous solution

分子量

antigen ~53 kDa

species reactivity

human

濃度

~2 mg/mL

アプリケーション

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.2-0.4 μg/mL using total cell extract of A431 cells

アイソタイプ

IgG2a

結合体

unconjugated

UniProtアクセッション番号

出荷済み

dry ice

保管温度

−20°C

Gene Information

human ... TP53(7157)

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詳細

Monoclonal Anti-p53 (mouse IgG2a isotype) is derived from hybridoma DO-1 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53. The p53 gene is located on human chromosome 17p. The gene codes for a tumor suppressor protein that is expressed in normal tissues.

特異性

Mouse anti-(p53) antibody reacts specifically with (p53) if human.

免疫原

recombinant human wild type p53. The epitope resides between amino acids 20-25 of human p53.

アプリケーション

Anti-p53 antibody, Mouse monoclonal has been used in:
  • semi-dry western blotting
  • immunoblotting
  • immunofluorescence
  • enzyme-linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • immunohistochemistry
  • immunoprecipitation
  • flow cytometry
  • chromatin immunoprecipitation (ChIP) assay

生物化学的/生理学的作用

The p53 tumor suppressor protein is important in the cellular response to DNA damage and other genomic aberrations. Elevation of p53 protein induces the transcriptional activation of multiple genes, including p21awf1. Wild-type p53 is shown to be a sequence-specific transcription factor, directly interacting with various cellular and viral proteins. Intact p53 function is essential for the maintenance of the non-tumorogenic phenotype of cells.p53 has a pivotal role in suppressing the development of cancer, cell cycle regulation and modulation of CAK kinase activity.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

ドイツ水質汚染分類

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

個人用保護具 (PPE)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

試験成績書(COA)

原産地証明書(COO)

Mikael S Lindström et al.
PloS one, 5(3), e9578-e9578 (2010-03-12)
Disruption of the nucleolus often leads to activation of the p53 tumor suppressor pathway through inhibition of MDM2 that is mediated by a limited set of ribosomal proteins including RPL11 and RPL5. The effects of ribosomal protein loss in cultured...
Knockdown of hnRNPK leads to increased DNA damage after irradiation and reduces survival of tumor cells
Wiesmann N, et al.
Carcinogenesis, 38(3), 321-328 (2017)
Lena Wedeken et al.
The Journal of biological chemistry, 286(50), 42855-42862 (2011-10-29)
The tumor suppressor protein Pdcd4 is thought to suppress translation of mRNAs containing structured 5'-UTRs by interacting with translation initiation factor eIF4A and inhibiting its helicase activity. However, natural target mRNAs regulated by Pdcd4 so far are mostly unknown. Here...
Maria Yusenko et al.
Scientific reports, 8(1), 13159-13159 (2018-09-05)
The transcription factor MYB plays key roles in hematopoietic cells and has been implicated the development of leukemia. MYB has therefore emerged as an attractive target for drug development. Recent work has suggested that targeting MYB by small-molecule inhibitors is...
Nadine Wiesmann et al.
Carcinogenesis, 38(3), 321-328 (2017-04-21)
Radiotherapy is an important treatment option in the therapy of multiple tumor entities among them head and neck squamous cell carcinoma (HNSCC). However, the success of radiotherapy is limited by the development of radiation resistances. Heterogeneous nuclear ribonucleoprotein K (hnRNPK)...
資料
p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.
さらに詳しく
p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.
さらに詳しく
Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.
さらに詳しく

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