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P7626

Sigma-Aldrich

フェニルメチルスルホニルフルオリド

≥98.5% (GC)

別名:
α-トルエンスルホニルフルオリド, PMSF, ベンジルスルホニルフルオリド, フェニルメチルスルホニルフルオリド
Empirical Formula (Hill Notation):
C7H7FO2S
CAS番号:
分子量:
174.19
Beilstein:
2088311
EC 番号:
MDL番号:
PubChem Substance ID:
NACRES:
NA.77

品質水準

300

由来生物

synthetic

アッセイ

≥98.5% (GC)

形態

powder

mp

91-94 °C

溶解性

dry solvents (ethanol, methanol, and 2-propanol): 200 mM (Stock solution are stable for months at 4°C.)
H2O: unstable

SMILES string

FS(=O)(=O)Cc1ccccc1

InChI

1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2

InChI key

YBYRMVIVWMBXKQ-UHFFFAOYSA-N

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詳細

Phenylmethanesulfonyl fluoride (PMSF) is a widely used serine protease inhibitor, effective against such enzymes as chymotrypsin, thrombin, and trypsin. PMSF acts as an inhibitor via sulfonation of the hydroxyl residues of serine residues at the reactive sites of serine proteases.

アプリケーション

PMSFは、細胞や組織の溶解後にタンパク質を分解するおそれのあるプロテアーゼを阻害することにより、タンパク質の単離中やサンプルの調製中に目的のタンパク質の保存を補助するための溶解バッファーによく使用されます。 PMSFは水性培地中では不安定です。 そのため、水性培地に使用する前に、PMSFの原液を無水有機溶媒(例えば、100%エタノールまたは無水イソプロパノール)で調製するのが一般的です。

PMSFには以下のような一般的特徴や利点があります:
  • トリプシンやキモトリプシンなどのセリンプロテアーゼを阻害します
  • また、システインプロテアーゼ(還元チオールによって可逆)や、哺乳類アセチルコリンエステラーゼも阻害します
  • DFPほどの効果もしくは毒性はありません
  • 有効濃度は0.1~1 mMです
  • 半減期はpH 7.5で1時間
フッ化フェニルメチルスルホニルは、以下のアプリケーションに使用されています:
  • 細胞分画。
  • 核タンパク質抽出における添加剤。
  • コレステロールエステラーゼ(CE)および偽性コリンエステラーゼ(PCE)の阻害剤。
  • 血漿 ANP レベルの定量のための遠心分離前の血液採取。

包装

250 mg in glass bottle
1, 5, 25, 100 g in glass bottle

生物化学的/生理学的作用

PMSFを投与すると、その抗コリンエステラーゼ作用と関連しない鎮痛が得られ、中枢に投与したβ-エンドルフィンの鎮痛作用が延長します。

ピクトグラム

CorrosionSkull and crossbones

シグナルワード

Danger

危険有害性情報

危険有害性の分類

Acute Tox. 3 Oral - Skin Corr. 1B

保管分類コード

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

個人用保護具 (PPE)

Eyeshields, Faceshields, Gloves, type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges

試験成績書(COA)

ロット番号を入力して試験成績書(COA)を検索してください。

原産地証明書(COO)

ロット番号を入力して原産地証明書(COO)を検索してください。

  1. How should Product P7626, Phenylmethanesulfonyl fluoride (PMSF), solutions be prepared?

    Stock solutions of 100-200 mM can be prepared in anhydrous isopropanol or anhydrous absolute ethanol. PMSF is unstable in the presence of water.

  2. How should stock solutions of Product P7626,  Phenylmethanesulfonyl fluoride (PMSF), be stored?

    A 200 mM solution in dry solvent is stable for at least 9 months when stored at 2-8°C.

  3. What does Product P7626,  Phenylmethanesulfonyl fluoride (PMSF), inhibit?

    PMSF is a serine protease inhibitor, inhibiting the activity of enzymes such as thrombin, trypsin, papain, chymotrypsin and proteinase K. It also inhibits mammalian acetylcholinesterases.

  4. What is the effective amount of Product P7626,  Phenylmethanesulfonyl fluoride (PMSF), to be used?

    The effective concentration range is 0.1-1 mM. A final concentration of 5 mM was used for proteinase K inhibition.

  5. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  6. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

C Pinsky et al.
Life sciences, 31(12-13), 1193-1196 (1982-09-20)
Intraperitoneal (IP) injection of the serine proteinase inhibitor phenylmethylsulfonyl fluoride (PMSF) produced dose-dependent analgesia in Sprague-Dawley rats. AD50 was 2.9 +/- 1.4 (S.E.) mg kg-1, the analgesia was antagonized by naloxone but unaffected by atropine. PMSF significantly enhanced the analgesic
Estrogen receptors activate atrial natriuretic peptide in the rat heart.
Jankowski M, et al.
Proceedings of the National Academy of Sciences of the USA, 98(20), 11765-11770 (2001)
Salivary esterase activity and its association with the biodegradation of dental composites.
Finer Y and Santerre JP.
Journal of Dental Research, 83(1), 22-26 (2004)
Lise Boussemart et al.
Nature, 513(7516), 105-109 (2014-08-01)
In BRAF(V600)-mutant tumours, most mechanisms of resistance to drugs that target the BRAF and/or MEK kinases rely on reactivation of the RAS-RAF-MEK-ERK mitogen-activated protein kinase (MAPK) signal transduction pathway, on activation of the alternative, PI(3)K-AKT-mTOR, pathway (which is ERK independent)
Rui Chen et al.
The Journal of biological chemistry, 287(36), 30800-30811 (2012-07-19)
Hypoxia-inducible factors (HIFs) are oxygen-sensitive transcription factors. HIF-1α plays a prominent role in hypoxic gene induction. HIF-2α target genes are more restricted but include erythropoietin (Epo), one of the most highly hypoxia-inducible genes in mammals. We previously reported that HIF-2α

資料

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プロトコル

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