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P8849

Sigma-Aldrich

プロテアーゼインヒビターカクテル

for use in purification of Histidine-tagged proteins, DMSO solution

MDL番号:
NACRES:
NA.77

品質水準

300

由来生物

synthetic

形態

DMSO solution

保管温度

−20°C

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特異性

セリン、システイン、アスパラギン酸、サーモリシン様プロテアーゼおよびアミノペプチダーゼを阻害します。

包装

1 mL in poly bottle
5 mL in glass bottle

アプリケーション

ヒスチジンタグ付きタンパク質の分離のために最適化され、検査済みです。IMAC用途への完全な適合性を確保するために、キレート剤は使用していません。

構成

AEBSF
ベスタチン
E-64
ペプスタチンA
ホスホラミドン

数量

20 gの Escherichia coliまたは10 gのバキュロウイルス感染Spodoptera frugiperda(ツマジロクサヨトウ)蛹卵巣細胞から総容量100 mLで得た溶液のプロテアーゼを阻害するには、1 mLを使用することを推奨します。

このプロテアーゼヒンヒビターカクテルはヒスチジン標識タンパク質用に最適化し、検査済みです

調製ノート

この製品は、DMSOを溶媒とする透明な溶液として供給します。 20 gのE. Coli細胞または10 gのバキュロウイルス感染細胞から得た100 mLの細胞ライセート中のプロテアーゼ活性の阻害には、1 mLの溶液を使用することを推奨します。

保管分類コード

10 - Combustible liquids

WGK

WGK 1

引火点(°F)

185.0 °F - closed cup

引火点(℃)

85 °C - closed cup

試験成績書(COA)

ロット番号を入力して試験成績書(COA)を検索してください。

原産地証明書(COO)

ロット番号を入力して原産地証明書(COO)を検索してください。

Quotes and Ordering

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the difference between Protease Inhibitors P8849 and S8830?

    P8849 is a DMSO solution and S8830 is a water soluble tablet. P8849 formulation is considered proprietary but does contain AEBSF, Pepstatin A, Bestatin, E-64, and Phosphoramidon. The formulation of S8830 also contains Aprotinin and Pepstatin A and the exact formulation is given on the product information sheet (under Documents, above).

  6. Can Product No. P8849, Protease Inhibitor Cocktail, be used with with HIS-Select products or any other immobilized metal affinity chromatography (IMAC) products?

    This product does not contain EDTA and can be used with sample processing by immobilized metal affinity chromatography (IMAC) suchas used for the purification of histidine-tagged proteins..

  7. How much of Product No. P8849, Protease Inhibitor Cocktail, should be added to the cell lysate?

    A 1:100 dilution is the recommended concentration.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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