P8999

Sigma-Aldrich

モノクロナール抗p53 マウス宿主抗体

clone DO-7, purified from hybridoma cell culture

別名:
抗TRP53抗体, 抗形質転換関連タンパク質53抗体
MDL番号:
NACRES:
NA.41

品質水準

200

由来生物

mouse

抗体製品の状態

purified immunoglobulin

antibody product type

primary antibodies

クローン

DO-7, monoclonal

形態

buffered aqueous solution

分子量

antigen ~53 kDa

species reactivity

human

濃度

~2 mg/mL

アプリケーション

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.1-0.2 μg/mL using human A431

アイソタイプ

IgG2b

結合体

unconjugated

UniProtアクセッション番号

出荷済み

dry ice

保管温度

−20°C

Gene Information

human ... TP53(7157)

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詳細

Monoclonal anti-p53, (mouse IgG2b isotype) is derived from the hybridoma DO-7 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53. The p53 gene is located on chromosome 17p. The p53 protein is highly conserved and expressed in normal tissues.

アプリケーション

Monoclonal Anti-p53 antibody produced in mouse has been used in:
  • immunoblotting
  • enzyme linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • immunohistochemistry
  • immunoprecipitation

生物化学的/生理学的作用

p53 is a transcription factor that regulates several critical cellular functions such as DNA repair, apoptosis, and cell cycle arrest. p53 primarily functions as a tumor suppressor and inactivates apoptosis inhibitors such as survivin, MCL-1 and BCL-2. Mutations in p53 gene occur most commonly in a wide range of human cancers. Thus, analysis of p53 function has important implications in cancer pathogenesis and therapy . Monoclonal Anti-p53 antibody specifically reacts with p53 in humans.
Elevation of p53 protein induces the transcriptional activation of multiple genes, including p21waf1. p21waf1 (cyclin-dependent kinase inhibitor 1A) interacts directly with cyclin dependent kinases, important for cell cycle progression, thereby inhibiting their activity and resulting in cell cycle arrest.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

ドイツ水質汚染分類

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

個人用保護具 (PPE)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

試験成績書(COA)

原産地証明書(COO)

DNA damage-induced activation of p53 by the checkpoint kinase Chk2
Hirao A, et al.
Science (New York, N.Y.), 287(5459), 1824-1827 (2000)
New Insights into p53 Signaling and Cancer Cell Response to DNA Damage: Implications for Cancer Therapy.
Mirzayans, R., et al.
Journal of Biomedicine and Biotechnology, doi: 10-doi: 10 (2012)
Predictors of progression in Barrett's esophagus II: baseline 17p (p53) loss of heterozygosity identifies a patient subset at increased risk for neoplastic progression
Reid BJ, et al.
The American Journal of Gastroenterology, 96(10), 2839-2839 (2001)
Nalini Mehta et al.
Molecular therapy oncolytics, 4, 1-17 (2017-03-28)
Treatment of aggressive glioblastoma brain tumors is challenging, largely due to diffusion barriers preventing efficient drug dosing to tumors. To overcome these barriers, bacterial carriers that are actively motile and programmed to migrate and localize to tumor zones were designed....
Ting Zhang et al.
International journal of molecular medicine, 40(1), 21-30 (2017-05-13)
Dihydroartemisinin (DHA) has been shown to inhibit the viability of various cancer cells. Previous studies have revealed that the mechanisms involved in the inhibitory effects of DHA are based on theactivation of p53 and the mitochondrial-related cell death pathway. However...
資料
p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.
さらに詳しく
p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.
さらに詳しく
Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.
さらに詳しく

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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