PCRISPR003

Human CRISPR Inhibition Dolcetto Library

• NACRES: NE.02
• UNSPSC Code: 41105904

特性

• packaging: pkg of 10 vials (5x200µL aliquots of each component)
• concentration: ≥5x108 VP/ml (via p24 Assay)
• application(s): CRISPR
• shipped in: dry ice
• storage temp.: −70°C

詳細

Application

• Functional Genomics/Target Validation
• Focused forward genetic activation screening
• Validated positive and negative controls
• Set up and optimization of screen assay
• Creation of cell lines stably expressing KRAB-dCas9

Biochem/physiol Actions

The power of CRISPR for genome engineering, coupled with the ability to perform large-scale, whole-genome loss-of-function (LOF) screening, has allowed breakthroughs in identifying gene pathways in drug resistance and disease. CRISPR is most commonly used to create double-stranded breaks that often result in loss of gene function (CRISPR-KO). However, the full extent of CRISPR′s utility extends beyond just targeted cutting of DNA. CRISPR′s Nuclease-independent applications provide equal targeting specificity. Instead of cutting, CRISPRi allows for targeted interference of gene function by delivering transcriptional repressor domains to a specific target sequence using modified dCas9 + gRNA complexes. Gene knockdown complements CRISPR-KO and CRISPRa (activation) and has distinct advantages over existing loss-of-function strategies like RNAi.

Features and Benefits

• Focus on your research, and we will generate your lentivirus screening library
• Compact and ready to use CRISPRi library ~6 sgRNAs
• Optimized (F+E) gRNA scaffold (pools are gRNA-only, KRAB-dCas9 sold separately)
• Can be paired with CRISPRICON to ease optimization using validated positive control targeting RAB1A and non-targeting control for assay setup
• UCOE KRAB-dCas9 for consistent effector expression across a wide variety of cell lines
• Ease of optimization: Utilizes BFP and Puromycin as selection markers under EF1alpha promoter.

General description

The Human CRISPR inhibition (CRISPRi) library (Dolcetto) is a single compact library that inhibits over 18,000 human genes and is used for genome-wide inhibition screening using a KRAB-dCas9 effector. The library is designed to be compact and efficient to maximize screening efficiency and performance, as published by Sanson, K.R., et al. Nat Commun 9, 5416 (2018).

Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.

Legal Information

CRISPR USE LICENSE AGREEMENT

LENTIVIRAL, WPRE AND EVROGEN FLUOROPHORE LICENSES

Other Notes

2 Subpools with a minimum concentration of 5x10⁸ VP/mL(via p24 assay)
PCRISPR003A - Human CRISPR Inhibition Dolcetto Library Set A
PCRISPR003B - Human CRISPR Inhibition Dolcetto Library Set B

安全性情報

• 保管分類: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable