The Brunello CRISPR library utilizes puromycin selection.
PCRISPR007
Human CRISPR Brunello Knockout Library
別名:
CRISPR Brunello Library, CRISPR Knockout Library, Knockout Library Kit
スキップする
packaging
pkg of 5 vials (5x200µL aliquots )
concentration
≥5x108 VP/ml (via p24 Assay)
application(s)
CRISPR
shipped in
dry ice
storage temp.
−70°C
1 of 4
当該品目 | PCRISPR004 | PCRISPR008 | PCRISPR006 |
|---|---|---|---|
| packaging pkg of 5 vials (5x200µL aliquots ) | packaging pkg of 5 vials (5x200µL aliquots ) | packaging pkg of 5 vials (5x200µL aliquots ) | packaging pkg of 5 vials (5x200µL aliquots ) |
| concentration ≥5x108 VP/ml (via p24 Assay) | concentration ≥5x108 VP/ml (via p24 Assay) | concentration ≥5x108 VP/ml (via p24 Assay) | concentration ≥5x108 VP/ml (via p24 Assay) |
| application(s) CRISPR | application(s) CRISPR | application(s) CRISPR | application(s) CRISPR |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. −70°C | storage temp. −70°C | storage temp. −70°C | storage temp. −70°C |
General description
The human CRISPR ′Brunello′ pooled library is designed using optimized metrics, as published by, Doench et al. Nat Biotechnol. (2016) and described further in Sanson, K.R., et al. Nat Commun (2018), which combine improved on-target activity predictions (Rule Set 2) with an off-target score, the Cutting Frequency Determination (CFD). The library is designed to be compact and efficient to maximize screening efficiency and performance.
Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.
Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.
Application
- Functional Genomics/Target Validation
- Unbiased wholed genome forward genetic screening
- Validated positive and negative controls
- Set up and optimization of screen assay
Biochem/physiol Actions
In a CRISPR KO screen, Cas9 introduces double-strand breaks at locations specified by a gRNA. When the endogenous non-homologous end-joining (NHEJ) DNA repair system corrects these breaks, this often leads to the introduction of frame-shift mutations that effectively knock out the gene. Thus, the power of CRISPR for genome engineering, coupled with the ability to perform large-scale, whole-genome loss-of-function (LOF) screening, has allowed breakthroughs in identifying gene pathways in drug resistance and disease.
Features and Benefits
- Focus on your research, and we will generate your lentivirus screening library
- Use CRISPR nucleases to knockout protein-coding genes to assess their function.
- Human Genes targeted 19,114
- Compact library of ~ four gRNAs per gene (76,441 total)
- Total Controls 1000(pools are gRNA-only, Cas9 sold separately) See products: LVCAS9BST or LVCAS9NEO for sources of Cas9
保管分類
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Cartagena Act Listed
cart
John G Doench et al.
Nature biotechnology, 34(2), 184-191 (2016-01-19)
CRISPR-Cas9-based genetic screens are a powerful new tool in biology. By simply altering the sequence of the single-guide RNA (sgRNA), one can reprogram Cas9 to target different sites in the genome with relative ease, but the on-target activity and off-target
資料
Deconvolute pooled shRNA samples post-screening to identify significant TRC clones with our service.
This screening guide covers how to choose a cell line, a screening library, and experimental conditions as well as tips for designing and performing your experiment.
このスクリーニングガイドには、細胞株、スクリーニングライブラリ、および実験条件の選択方法と、実験を計画および実行するためのヒントが記載されています。
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）