SRP3164

sTRAIL レセプター1 (DR4) ヒト

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• 別名: Apo2, DR4, TNFRSF10A, 可溶性TRAIL レセプター1

特性

• 由来生物: human
• リコンビナント: expressed in E. coli
• アッセイ: ≥98% (HPLC); ≥98% (SDS-PAGE)
• 形状: lyophilized
• 有効性: 100 ng/mL
• 分子量: 22.7 kDa
• 包装: pkg of 50 μg
• technique(s): cell culture | mammalian: suitable
• 不純物: <0.1 EU/μg endotoxin, tested
• 色: white
• UniProtアクセッション番号: O00220
• 輸送温度: wet ice
• 保管温度: −20°C
• Gene Information: human ... TNFRSF10A(8797)

詳細

詳細

Soluble tumor necrosis factor-related apoptosis-inducing ligand-receptor 1 (sTRAIL-R1) also known as DR4, is encoded by the gene mapped to human chromosome 8p21-22. Recombinant human soluble TRAIL Receptor-1/DR4 is a 22.7kDa protein (216 amino acid residues) consisting of the TNFR homologous, cysteine rich portion of the extracellular domain. TRAIL Receptor-1/DR4 and TRAIL Receptor-2/DR5 belong to the TNFR superfamily of transmembrane proteins. TRAIL-R1 contains a cytoplasmic "death domain," which can activate the cell′s apoptotic machinery. TRAIL Receptor-1/DR4 is activated by binding to either membrane anchored or soluble TRAIL/Apo2L.

生物化学的／生理学的作用

Soluble tumor necrosis factor-related apoptosis-inducing ligand-receptor 1 (sTRAIL-R1) interacts with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and stimulate cell-death signaling pathway. Mutation of the sTRAIL-R1 gene is associated with the pathogenesis of non-Hodgkin′s lymphoma (NHL). In addition, variation of TRAIL-R1 also increases the risk of susceptibility to prostate cancer (PCa) in North Indian population.

シーケンス

MSGTGAAAAT PSKVWGSSAG RIEPRGGGRG ALPTSMGQHG PSARARAGRA PGPRPAREAS PRLRVHKTFK FVVVGVLLQV VPSSAATIKL HDQSIGTQQW EHSPLGELCP PGSHRSERPG ACNRCTEGVG YTNASQQLFA CLPCTACKSD EEERSPCTTT RNTACQCKPG TFRNDNSAEM CRKCSTGCPR GMVKVKDCTP WSDIECVHKE SGNGHN

物理的形状

凍結乾燥品(添加物不含)。

再構成

バイアルは、開封前に遠心してください。0.1～1.0 mg/mLの濃度となるよう水で再構成してください。ボルテックスは行わないでください。この溶液は2～8°Cで1週間まで保存できます。長期保存の際は、キャリアタンパク質(0.1% BSAなど)を含有するバッファーで希釈し、1回使用量ごとに分けて-20～-80°Cで保存することをお勧めします。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable