SRP3300

Sigma-Aldrich

HGF mouse

recombinant, expressed in Hi-5 Insect cells, ≥95% (SDS-PAGE), ≥95% (HPLC)

別名:
Hepatopoietin (HPTA), Scatter Factor (SF)
NACRES:
NA.32

由来生物

mouse

リコンビナント

expressed in Hi-5 Insect cells

アッセイ

≥95% (HPLC)
≥95% (SDS-PAGE)

形態

lyophilized

有効性

10-20 ng/mL

分子量

80.0 kDa

包装

pkg of 20 μg

不純物

<0.1 EU/μg endotoxin, tested

white to off-white

UniProtアクセッション番号

出荷済み

wet ice

保管温度

−20°C

Gene Information

mouse ... HGF(15234)

関連するカテゴリー

詳細

Hepatocyte growth factor (HGF) is a plasmin related disulfide-linked, hetereodimeric protein containing an α and a β chain. It is synthesized as inactive, single-chain precursor which shares similarity with plasminogen. Its α chain contains an N-terminal hairpin and four kringle domains (NK4).

生物化学的/生理学的作用

Hepatocyte growth factor (HGF) functions via its receptor c-Met, which gets activated upon tyrosine phosphorylation, and subsequently promotes epithelial cell proliferation, morphogenesis, angiogenesis and motility. It plays a crucial role in organ development, as inactivation of either this gene or its receptor in fetal stages results in organ hypoplasia. Endogenously this protein is involved in the self-repair of various ograns including injured livers, kidneys, lungs etc. HGF is a predominant potent factor involved in lung growth and repair. It functions as a motogen, tubulogen and anti-apoptotic agent on airway epithelial and other cells expressing HGF receptor c-Met. Studies in mice show that HGF determines the severity of Pseudomonas aeruginosa keratitis by enhanced HGF signaling through c-met resulting in elevated pro-inflammatory cytokines and reduced anti-inflammatory cytokines.

物理的形状

Lyophilized from 10 mM Tris, pH 7.2 + 100mM L-Arginine + 200 mM NaCl.

再構成

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Note: Slow to dissolve. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

RIDADR

NONH for all modes of transport

ドイツ水質汚染分類

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

試験成績書(COA)
原産地証明書(COO)
Regulation of hepatocyte growth factor in mice with pneumonia by peptidases and trans-alveolar flux.
Raymond WW et al
PLoS ONE, 10(5), e0125797-e0125797 (2015)
HGF signaling impacts severity of Pseudomonas aeruginosa keratitis.
Jiang X et al
Investigative Ophthalmology & Visual Science, 55(4), 2180-2190 (2014)
L Naldini et al.
The EMBO journal, 10(10), 2867-2878 (1991-10-01)
Scatter Factor (SF) is a fibroblast-secreted protein which promotes motility and matrix invasion of epithelial cells. Hepatocyte Growth Factor (HGF) is a powerful mitogen for hepatocytes and other epithelial tissues. SF and HGF, purified according to their respective biological activities...
Rosaria Gangemi et al.
Pigment cell & melanoma research, 27(6), 1138-1148 (2014-08-16)
Uveal melanoma (UM) is a rare ocular tumor that may lead to deadly metastases in 50% of patients. A disintegrin and metalloproteinase (ADAM)10, ADAM17, and the HGF-receptor c-Met support invasiveness in different tumors. Here, we report that high ADAM10, MET...
Jian-Ya Zhou et al.
Cancer letters, 351(2), 265-271 (2014-07-02)
In non-small-cell lung cancer (NSCLC) that harbours an activating epidermal growth factor receptor (EGFR) mutation, over-expression of hepatocyte growth factor (HGF) is an important mechanism involved in the acquired resistance to EGFR-tyrosine kinase inhibitors (TKIs) by restoring activity of the...

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