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  • SRP5085
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SRP5085

Sigma-Aldrich

SIK (1-303)、活性型、Hisタグ融合 ヒト

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):
MSK, SIK1, SNF1LK
NACRES:
NA.32

リコンビナント

expressed in baculovirus infected Sf9 cells

製品種目

PRECISIO® Kinase

アッセイ

≥70% (SDS-PAGE)

形状

buffered aqueous glycerol solution

specific activity

296-401 nmol/min·mg

分子量

~36 kDa

NCBIアクセッション番号

輸送温度

dry ice

保管温度

−70°C

Gene Information

human ... SIK1(150094)

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This Item
SRP5004SRP5003P0060
recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

specific activity

296-401 nmol/min·mg

specific activity

255-345 nmol/min·mg

specific activity

238-322 nmol/min·mg

specific activity

14-20 nmol/min·mg

mol wt

~36 kDa

mol wt

~36 kDa (B2), ~65 kDa (G2), ~69 kDa (A2)

mol wt

~36 kDa (B2), ~40 kDa (G1), ~69 kDa (A2)

mol wt

~70 kDa

NCBI accession no.

NM_173354

NCBI accession no.

NM_001040633, NM_005399, NM_006252

NCBI accession no.

NM_002733, NM_005399, NM_006252

NCBI accession no.

-

詳細

SIKは、AMPK関連キナーゼの調節に関与するプロテインキナーゼです。SIKは、LKB1の腫瘍抑制因子としての機能など、生理的な作用を仲介するとみられています。また、AMPKに関連するSTRAD、NUAK1、NUAK2、BRSK1、BRSK2、QIK、QSK、SIK、MARK1、MARK2、MARK3、MARK4、MELKのような様々なタンパク質によるシグナル伝達に関与しています。SIK1がthr322でリン酸化を受けて活性化されると、細胞膜におけるナトリウム/カリウムATPアーゼαサブユニットの触媒活性が上昇します。この結果、インタクトな哺乳動物細胞で細胞内ナトリウムの上昇が認められています。

物理的形状

50 mMリン酸ナトリウム(pH 7.0, 300 mM NaCl, 150 mMイミダゾール, 0.1 mM PMSF, 0.25 mM DTT, 25%グリセロール含有)を用いた溶液として調製されています。

調製ノート

開封後はそれぞれの目的に応じて少量ずつ分注し、-70°Cで保存してください。また、取扱いや凍結融解について、繰り返しのご使用は避けてください。

法的情報

PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC

ピクトグラム

Exclamation markHealth hazard

シグナルワード

Danger

危険有害性情報

危険有害性の分類

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

保管分類コード

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

名称等を表示すべき危険物及び有害物

ISHL Notified Names

名称等を通知すべき危険物及び有害物

Cartagena Act

カルタヘナ法


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