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T5393

Sigma-Aldrich

抗マウスIgG (全分子)−TRITC ヤギ宿主抗体

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):
Goat Anti-Mouse IgG (whole molecule)−Tetramethylrhodamine isothiocyanate
MDL番号:
NACRES:
NA.46

由来生物

goat

品質水準

200

結合体

TRITC conjugate

抗体製品の状態

IgG fraction of antiserum

antibody product type

secondary antibodies

クローン

polyclonal

形態

buffered aqueous solution

technique(s)

direct immunofluorescence: 1:64

保管温度

2-8°C

Related Categories

詳細

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Anti-Mouse IgG (whole molecule)-TRITC antibody is specific for mouse IgG and all subclasses, it cross reacts with mouse IgA and IgM. Antibody purification is by ion exchange chromatography after which it is conjugated to crystalline tetramethylrhodamine isothiocyanate (TRITC).
Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other goat serum proteins. The antibody is conjugated to crystalline tetramethylrhodamine isothiocyanate (TRITC) and then further purified to remove free TRITC. Specificity for mouse IgG is determined by Ouchterlony Double Diffusion (ODD). The antibody preparation is specific for mouse IgG and all subclasses, it cross reacts with mouse IgA and IgM. Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion against anti-goat IgG and anti-goat whole serum results in single arcs of precipitation in the γ region.

特異性

The antibody preparation is specific for mouse IgG and all subclasses, it cross reacts with mouse IgA and IgM.

免疫原

IgG isolated from pooled normal mouse serum

アプリケーション

Anti-Mouse IgG (whole molecule)-TRITC antibody may be used for immunofluorescence at a minimum antibody dilution of 1:64 using mouse spleen cells. Antibody dilution range of 1:50 to 1:250 was used for immunofluorescence in various studies.

包装

0.5 mL in glass insert
1, 2 mL in glass bottle

物理的形状

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

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