T8154

Sigma-Aldrich

トリパンブルー 溶液

0.4%, liquid, sterile-filtered, suitable for cell culture

Empirical Formula (Hill Notation):
C34H24N6Na4O14S4
CAS番号:
分子量:
960.81
Beilstein/REAXYS Number:
4360496
MDL番号:
PubChem Substance ID:
NACRES:
NA.75

品質水準

200

無菌性

sterile-filtered

形態

liquid

保管条件

dry at room temperature

濃度

0.4%

アプリケーション

cell analysis: suitable
cell culture | mammalian: suitable
tissue processing: suitable

出荷済み

ambient

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

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詳細

トリパンブルーは、生細胞と死細胞の区別に使用される色素です。健康な生細胞では吸収されない生体染色ですが、細胞膜の損傷した細胞は染色されます。このように、死細胞のみを計測することができます。本法は色素排除法と呼ばれることもあります。

アプリケーション

細胞毒性アッセイ、細胞生存率のルーチン評価、および増殖アッセイにおいて、死細胞および瀕死細胞の検出に使用してください。

調製ノート

0.81% 塩化ナトリウムと0.06% リン酸水素二カリウムで調製されています。

ピクトグラム

Health hazard

シグナルワード

Danger

危険有害性情報

hazcat

Carc. 1B

storage_class_code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

ドイツ水質汚染分類

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

個人用保護具 (PPE)

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

試験成績書(COA)

原産地証明書(COO)

その他のドキュメント

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. Do the cells need to be washed to remove the culture media prior to staining with Product T8154, Trypan Blue solution?

    Trypan Blue has a greater affinity for serum proteins than for cellular protein. If the background is too dark, cells should be pelleted and resuspended in protein-free medium or salt solution prior to counting.

  3. Why is Trypan blue, Product T8154, used to determine cell viability?

    Trypan Blue is a vital stain recommended for use in estimating the proportion of viable cells in a population. The reactivity of this dye is based on the fact that the chromophore is negatively charged and does not react with the cell unless the membrane is damaged. Staining facilitates the visualization of cell morphology. Live (viable) cells do not take up the dye and dead (non-viable) cells do.

  4. Can Trypan blue, Product T8154, be used to discriminate between apoptotic and necrotic cells?

    Trypan blue will stain cells that have disrupted membranes.  It cannot differentiate between permeabilized membranes caused by apoptosis or necrosis.

  5. Trypan blue, Product T8154, contains a precipitate.  Is it still good to use?

    The trypan blue solution is still good to use.  It can be warmed to dissolve the precipitate or it can be filtered to remove the precipitate.

  6. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. Why are there red fibers in Product T8154, Trypan blue solution?

    A report of fibers in Product T8154, Trypan blue solution is sufficiently common almost to be a characteristic of the product. These fibers are a cosmetic fault rather than one of functionality.  The presence of fibers may be more pronounced following cold storage or shipping.  This product should be stored at room temperature. Filtration to remove the fibers will not affect the use of the product.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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