As mentioned on the Product Detail Page at the DESCRIPTION section, under 'Preparation Note' , this product is prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic.
T8154
トリパンブルー 溶液
0.4%, liquid, sterile-filtered, suitable for cell culture
別名:
Blue cell viability dye
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サイズを選択してください
20 ML
￥3,360
100 ML
￥5,140
￥3,360
本日発送予定詳細
この商品について
実験式（ヒル表記法）:
C34H24N6Na4O14S4
CAS番号:
分子量:
960.81
UNSPSC Code:
12352207
NACRES:
NA.75
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
4360496
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製品名
トリパンブルー 溶液, 0.4%, liquid, sterile-filtered, suitable for cell culture
form
liquid
InChI
1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
SMILES string
[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
InChI key
GLNADSQYFUSGOU-UHFFFAOYSA-J
sterility
sterile-filtered
storage condition
dry at room temperature
concentration
0.4%
technique(s)
cell culture | mammalian: suitable
tissue processing: suitable
application(s)
cell analysis
shipped in
ambient
Quality Level
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当該品目 | T6146 | 93595 | 302643 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable, tissue processing: suitable | technique(s) cell culture | mammalian: suitable, tissue processing: suitable | technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable |
| concentration 0.4% | concentration - | concentration 0.4% | concentration - |
| application(s) cell analysis | application(s) cell analysis | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| form liquid | form powder | form liquid | form powder |
| shipped in ambient | shipped in - | shipped in - | shipped in - |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
Application
General description
トリパンブルーは、生細胞と死細胞の区別に使用される色素です。健康な生細胞では吸収されない生体染色ですが、細胞膜の損傷した細胞は染色されます。[5]このように、死細胞のみを計測することができます。本法は色素排除法と呼ばれることもあります。
Preparation Note
0.81% 塩化ナトリウムと0.06% リン酸水素二カリウムで調製されています。
Comparable product
signalword
Danger
hcodes
Hazard Classifications
Carc. 1B
保管分類
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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Yu Deng
Journal of Biological Engineering, 11(2) (2017)
Vital dyes in vitreoretinal surgery: chromovitrectomy
Meyer CH
Developments in Ophthalmology, 42 (2008)
Roya Khosravi-Far, Zahra Zakeri
Programmed Cell Death (2008)
The sensitivity of 5-formylcytosine to doxorubicin regardless of DNA damage
Selcen
Turkiye Entomoloji Dergisi (Turkish Journal of Entomology), 40 (2016)
S R Mishra et al.
Research in veterinary science, 108, 98-111 (2016-09-25)
The present study investigated the expression and localization of FGF and its functional receptors in the follicle of buffalo and the treatment of FGF2 on mRNA expression of CYP19A1 (aromatase), PCNA, and BAX (BCL-2 associated X protein) in cultured buffalo
資料
Cell counting protocol using a hemocytometer for monitoring cell viability. Discover other cell counting tools such as the Scepter™ 3.0 Cell Counter.
肺オルガノイドは、ヒトの肺の発生や呼吸器疾患の研究において有用な3次元（3D）モデルです。3dGRO®分化プロトコルは、4つのステップでヒトiPSCからオルガノイドを生成します。
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.
プロトコル
Learn how to cultivate similar-sized iPSC-derived colon organoids using Millicell® Microwell plates and perform a forskolin-induced swelling assay.
Trypsin is commonly used for dissociating adherent cells from surfaces. A wide variety of trypsin solutions are available to meet your specific cell line requirements.
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
Step-by-step protocol for ADCC assays using cryopreserved NK effector cells including tips and tricks, assay development, and data analysis methods.
関連コンテンツ
Three-dimensional (3D) printing of biological tissue is rapidly becoming an integral part of tissue engineering.
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is this solution dissolved in PBS?
1 回答-
役に立ちましたか？
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What is the stability of the product. How long we can use it as there is no expiry date mentioned in the COA
1 回答-
This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
役に立ちましたか？
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Trypan blue, Product T8154, contains a precipitate. Is it still good to use?
1 回答-
The trypan blue solution is still good to use. It can be warmed to dissolve the precipitate or it can be filtered to remove the precipitate.
役に立ちましたか？
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Why is Trypan blue, Product T8154, used to determine cell viability?
1 回答-
Trypan Blue is a vital stain recommended for use in estimating the proportion of viable cells in a population. The reactivity of this dye is based on the fact that the chromophore is negatively charged and does not react with the cell unless the membrane is damaged. Staining facilitates the visualization of cell morphology. Live (viable) cells do not take up the dye and dead (non-viable) cells do.
役に立ちましたか？
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
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Do the cells need to be washed to remove the culture media prior to staining with Product T8154, Trypan Blue solution?
1 回答-
Trypan Blue has a greater affinity for serum proteins than for cellular protein. If the background is too dark, cells should be pelleted and resuspended in protein-free medium or salt solution prior to counting.
役に立ちましたか？
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Why are there red fibers in Product T8154, Trypan blue solution?
1 回答-
A report of fibers in Product T8154, Trypan blue solution is sufficiently common almost to be a characteristic of the product. These fibers are a cosmetic fault rather than one of functionality. The presence of fibers may be more pronounced following cold storage or shipping. This product should be stored at room temperature. Filtration to remove the fibers will not affect the use of the product.
役に立ちましたか？
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Can Trypan blue, Product T8154, be used to discriminate between apoptotic and necrotic cells?
1 回答-
Trypan blue will stain cells that have disrupted membranes. It cannot differentiate between permeabilized membranes caused by apoptosis or necrosis.
役に立ちましたか？
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