TRACRRNAMOD
SygRNA™ Cas9 Synthetic Modified tracrRNA
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|---|---|
5 nmol | カートの在庫状況を確認する | ￥11,500 |
この商品について
NACRES:
NA.51
UNSPSC Code:
12352208
packaging
pkg of 1 vial
shipped in
ambient
storage temp.
−20°C
General description
The SygRNA modified synthetic RNA system recruits WT SpCas9, eSpCas9, dCas9, or Cas9D10A to specific genomic targets. 2′ O-methyl and phosphothioate modifications have been incorporated to enhance the stability and performance of the synthetic RNA. The modified tracrRNA (trans-activating crRNA) binds to a crRNA (CRISPR RNA) and the resulting crRNA-tracrRNA complex guides SpCas9 and SpCas9 variant proteins to crRNA-specified sites in the genome. SygRNA modified synthetic tracrRNA and crRNAs are optimized for use with a variety of Cas9 expressing cell lines, Cas9 proteins, or Cas9 mRNAs and are compatible with a delivery methods such as microinjection, lipofection, and electroporation.
Application
Functional Genomics/Target Validation/Transgenics
- Creation of gene knockouts in multiple cell lines
- Complete knockout of genes not amenable to RNAi
- Creation of knock-in cell lines with promoters, fusion tags, or reporters integrated into endogenous genes
- Generation of transgenic animals via embryo microinjection or electroporation
Features and Benefits
HPLC-purified SygRNA modified tracrRNA oligo is optimized for use with a target-specific crRNA or modified crRNA and a source of SpCas9 protein for genome editing applications.
Preparation Note
Resuspend SygRNA modified tracrRNA oligo in a RNAase-free Tris-containing buffer with a pH 8.0 or less.
Other Notes
The SygRNA Cas9 tracrRNA oligo is used in conjunction with a target-specific crRNA and a source of Cas9 protein to form active RNP complexes.
Visit SigmaAldrich.com/CRISPRessentials to to review available Cas9 sources.
Order crRNAs at SigmaAldrich.com/CRISPR
Visit SigmaAldrich.com/CRISPRessentials to to review available Cas9 sources.
Order crRNAs at SigmaAldrich.com/CRISPR
1 of 1
当該品目 | |||
|---|---|---|---|
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in dry ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| packaging pkg of 1 vial | packaging pkg of 1 vial | packaging pkg of 5 nmol × Tube (HPLC) | packaging vial of 50 μL |
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable