ZMS1028

Sigma-Aldrich

Anti-α-Dystroglycan Antibody, clone VIA4-1 ZooMAb® Mouse Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

別名:
Dystrophin-associated glycoprotein 1

品質水準

200

由来生物

mouse

リコンビナント

expressed in HEK 293 cells

抗体製品の状態

purified antibody

antibody product type

primary antibodies

クローン

VIA4-1, monoclonal
recombinant monoclonal

製品ライン

ZooMAb® learn more

形態

lyophilized

分子量

calculated mol wt 97.44 kDa
observed mol wt ~140 kDa

species reactivity

rabbit, mouse, human

species reactivity (predicted by homology)

rat, guinea pig, canine

包装

antibody small pack of 25 μL

環境により配慮した代替製品の特徴

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

強化検証

recombinant expression
Learn more about Antibody Enhanced Validation

アプリケーション

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

アイソタイプ

IgG1

結合体

unconjugated

環境により配慮した代替製品カテゴリ

Aligned

出荷済み

ambient

保管温度

2-8°C

詳細

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特異性

CloneVIA4-1 is a ZooMAb mouse recombinant monoclonal antibody that specifically detects alpha-Dystroglycan.

免疫原

Rabbit skeletal muscle membrane preparations.

アプリケーション

Anti-a-Dystroglycan, clone VIA4-1 ZooMAb, Cat. No. ZMS1028, is a recombinant Mouse monoclonal antibody that targets a-Dystroglycan and is tested for use in Immunocytochemistry, Immunohisttochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected -Dystroglycan in mouse placenta and human heart tissue lysates.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected -Dystroglycan in HeLa and NIH 3T3 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected -Dystroglycan in rabbit skeletal muscle and human skeletal muscle tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

ターゲットの説明

Dystroglycan (UniProt: Q28685; also known as Dystrophin-associated glycoprotein 1) is encoded by the DAG1 gene (Gene ID: 100009278) in rabbit. Dystroglycan is a component of the dystrophin glycoprotein complex that serves as an adhesion molecule and links the extracellular matrix to the intracellular actin cytoskeleton. It is synthesized with a signal peptide (aa 1-29) that is subsequently cleaved off to generate the mature protein that is further autolytically cleaved (between 653-654) to produce alpha-dystroglycan (aa 30-653) and beta-dystroglycan (aa 654-895). Alpha-dystroglycan is an extracellular peripheral glycoprotein that binds to several extracellular matrix and synaptic proteins such as laminin, agrin, neurexin, and pikachurin. It is reported that its O-mannosyl glycosylation is required for its ligand- binding functions. Beta-dystroglycan is a single-pass type 1 transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton and serves as a cell adhesion receptor in both muscle and non-muscle tissues. In the cell, beta-dystroglycan binds to dystrophin that is linked to actin cytoskeleton. Hence, alpha/beta dystroglycans act as a molecular axis connecting extracellular matrix with the cytoskeleton across the plasma membrane. Defects in O-mannosyl glycan have been linked to various congenital muscular dystrophies caused by aberrant alpha-dystroglycan glycosylation. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Sudo, A., et al. (2018). Human Mol. Genetics. 27(7); 7 1174-1185; Street, RM., et al. (2012). Reproductive Sci. 19(3); 282-289; Kanagawa, M., et al. (2010). J. Biol. Chem. 285(41); 31208-31216).

物理的形状

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

再構成

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法的情報

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

ドイツ水質汚染分類

WGK 1

試験成績書(COA)
原産地証明書(COO)

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