ZRB1219

Sigma-Aldrich

Anti-MBP Antibody, clone 2N6 , ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

別名:
Myelin basic protein, Myelin membrane encephalitogenic protein, Myelin A1 protein

由来生物

rabbit (recombinant)

リコンビナント

expressed in HEK 293 cells

抗体製品の状態

purified antibody

antibody product type

primary antibodies

クローン

2N6, recombinant monoclonal

製品ライン

ZooMAb® learn more

形態

lyophilized

分子量

calculated mol wt 17.34 kDa
calculated mol wt 33.11  kDa
observed mol wt 18-20 kDa

species reactivity

human, rat, mouse

包装

pkg of 25 μL

環境により配慮した代替製品の特徴

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

強化検証

recombinant expression
Learn more about Antibody Enhanced Validation

アプリケーション

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

アイソタイプ

IgG

結合体

unconjugated

環境により配慮した代替製品カテゴリ

Aligned

出荷済み

ambient

保管温度

2-8°C

詳細

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特異性

Clone 2N6 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Myelin Basic Protein (MBP). It targets an epitope within 19 amino acids from the C-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal half of human Myelin Basic Protein.

アプリケーション

Anti-MBP, clone 2N6 ZooMAb, Cat. No. ZRB1219, is a highly specific recombinant rabbit monoclonal antibody that detects Myelin basic protein and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MBP in mouse and rat brain tissue lysate.

Immunocytochemistry Analysis: A 1:10 dilution from a representative lot detected MBP in Rat Glial precursor cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MBP in human brain and mouse brain tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary.

ターゲットの説明

Myelin basic protein (UniProt: P02686; also known as MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein) is encoded by the MBP gene (Gene ID: 4155) in human. MBP is a homodimeric protein that is found in both the central and the peripheral nervous system. It is one of the most abundant protein components of the myelin membrane in the CNS and plays a role in both the formation and stabilization of this myelin membrane. It is responsible for adhesion of the cytosolic surfaces of multi-layered compact myelin. MBP can interact with many polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and with negatively charged lipids. MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. MBP is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. MBP expression begins abruptly in 14-16-week-old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane that can lead to demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating Marburg disease. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

物理的形状

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

再構成

30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法的情報

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

ドイツ水質汚染分類

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable

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原産地証明書(COO)

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