ZRB1856

Sigma-Aldrich

Anti-14-3-3 β/α Antibody, clone 1D15 ZooMAb® Rabbit Monoclonal

newenhanced validation

recombinant, expressed in HEK 293 cells

由来生物

rabbit

品質水準

200

リコンビナント

expressed in HEK 293 cells

抗体製品の状態

purified antibody

antibody product type

primary antibodies

クローン

1D15, monoclonal
recombinant monoclonal

製品ライン

ZooMAb® learn more

形態

lyophilized

分子量

calculated mol wt ~ 28.08 kDa
observed mol wt ~ 28 kDa

species reactivity

human, monkey

species reactivity (predicted by homology)

sheep, bovine, rat

包装

antibody small pack of 25 μL

強化検証

recombinant expression
Learn more about Antibody Enhanced Validation

アプリケーション

ELISA: suitable
affinity binding assay: suitable
western blot: suitable

アイソタイプ

IgG

結合体

unconjugated

出荷済み

ambient

保管温度

2-8°C

詳細

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1D15 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects 14-3-3 protein β/a. It targets an epitope within 19 amino acids from the C-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal half of human 14-3-3 protein β/a.

アプリケーション

Anti-14-3-3 β/a, clone 1D15 ZooMAb®, Cat. No. ZRB1856, is a recombinant Rabbit monoclonal antibody that detects 14-3-3 β/a and is tested for use in Affinity Binding Assay, ELISA, and Western Blotting.
Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected 14-3-3 β/a in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected 14-3-3 β/a in SHSY5Y and COS-1 cell lysates.

ELISA Analysis: 1.0 μg/mL from a representative lot detected recombinant 14-3-3 β/a.

Affinity Binding Assay: A representative lot of this antibody bound 14-3-3 b/a peptide with a KD of 2.3 x 10-6 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

ターゲットの説明

14-3-3 protein β/a (UniProt: P31946; also known as Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1) is encoded by the YWHAB gene (Gene ID: 7529) in human. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and heterodimers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 protein beta/alpha serves as an adapter protein that has been implicated in the regulation of a large spectrum of both general and specialized signaling pathways. It binds to many partners, usually by recognition of a phosphoserine or phosphothreonine motif and its binding generally results in the modulation of the activity of the binding partner. The alpha, brain-specific form, differs from the beta form in its ability to be phosphorylated on serine 60 by protein kinase C delta in a sphingosine-dependent fashion. 14-3-3 β/a is shown to block the nuclear translocation of the phosphorylated form of SR protein-specific kinase 2 (SRPK2) and antagonize its stimulatory effect on cyclin D1 expression that results in blockage of SRPK2 elicited neuronal apoptosis. It also reported to serve as a negative regulator of osteogenesis. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

物理的形状

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

再構成

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法的情報

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

ドイツ水質汚染分類

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable

試験成績書(COA)
原産地証明書(COO)

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

テクニカルサービス連絡先

ソーシャルメディア

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

Merck

研究、開発、製造

私たちは、バイオテクノロジーと医薬品の研究&開発および製造のソリューションとサービスを提供する、グローバルなライフサイエンス業界のリーディングサプライヤーです。

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

当ウェブサイトのいかなる内容についても、許可なく複写することを禁じます。