ZRB1863

Sigma-Aldrich

Anti-Doublecortin Antibody, clone 3F11 ZooMAb® Rabbit Monoclonal

newenhanced validation

recombinant, expressed in HEK 293 cells

由来生物

rabbit

品質水準

200

リコンビナント

expressed in HEK 293 cells

抗体製品の状態

purified antibody

antibody product type

primary antibodies

クローン

3F11, monoclonal
recombinant monoclonal

製品ライン

ZooMAb® learn more

形態

lyophilized

分子量

calculated mol wt ~ 40.57 kDa
observed mol wt ~ 42 kDa

species reactivity

human, mouse

species reactivity (predicted by homology)

rat

包装

antibody small pack of 25 μL

強化検証

recombinant expression
Learn more about Antibody Enhanced Validation

アプリケーション

immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

アイソタイプ

IgG

結合体

unconjugated

出荷済み

ambient

保管温度

2-8°C

詳細

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

特異性

Clone 3F11 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Doublecortin. It targets an epitope within 17 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Doublecortin.

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Doublecortin in SH-SY5Y cell lysate.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Doublecortin in mouse brain tissue sections.

Immunocytochemistry Analysis: A 1:10 dilution from a representative lot detected Doublecortin in Neuro-2a and U-87 MG cells.

Immunofluorescence Analysis: A 1:10 dilution from a representative lot detected Doublecortin in frozen mouse brain tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Doublecortin, clone 3F11 ZooMAb®, Cat. No. ZRB1863, is a recombinant Rabbit monoclonal antibody that detects Doublecortin and tested for use in Western Blotting, Immunofluorescence, Immunocytochemistry, and Immunohistochemistry (Paraffin).

ターゲットの説明

Neuronal migration protein doublecortin (UniProt: O43602; also known as Doublin,Lissencephalin-X, Lis-X) is encoded by the DCX (also known as DBCN, LISX) gene (Gene ID: 1641) in human. Doublecortin is a microtubule-associated protein that is required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. It is expressed almost exclusively in immature neurons. Hence, it has emerged as an important marker of neurogenesis. It is highly expressed in newly produced cells in the neurogenic zones; the sub ventricular zone along the lateral ventricle and the subgranular zone (SGZ) of the dentate gyrus. Doublecortin binds to microtubules in migrating cells and as a result promote the movement of these cells. It is reported to direct neuronal migration by regulating the organization and stability of microtubules. Doublecortin may act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein and may participate in a signaling pathway that is crucial for neuronal interaction before and during migration. It undergoes phosphorylation by MARK1, MARK2, and PKA and the phosphorylation regulates its ability to bind microtubules. Doublecortin is reported to be involved in cancer metastasis where unstable interactions between doublecortin and microtubules destabilize cytoskeletal organization leading to disorganized movements of cells that can lead to the uncontrolled migration of cancer cells. Mutations in DCX gene are reported to cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, cognitive disability, subcortical band heterotopia. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

物理的形状

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

再構成

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法的情報

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

ドイツ水質汚染分類

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable

試験成績書(COA)
原産地証明書(COO)

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

テクニカルサービス連絡先

ソーシャルメディア

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

Merck

研究、開発、製造

私たちは、バイオテクノロジーと医薬品の研究&開発および製造のソリューションとサービスを提供する、グローバルなライフサイエンス業界のリーディングサプライヤーです。

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

当ウェブサイトのいかなる内容についても、許可なく複写することを禁じます。