While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
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製品名
アセンティス®エクスプレス C8, 2.7 μm HPLCカラム, 2.7 μm particle size, L × I.D. 10 cm × 2.1 mm
物質
stainless steel column
品質水準
認証
suitable for USP L7
製品種目
Ascentis®
特徴
endcapped
メーカー／製品名
Ascentis®
包装
1 ea of
パラメーター
60 °C temp. range
600 bar max. pressure (9000 psi)
テクニック
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
L × 内径
10 cm × 2.1 mm
表面積
135 m2/g
不純物
<5 ppm metals
Matrix
Fused-Core particle platform
superficially porous particle
マトリックス活性基
C8 (octyl) phase
粒径
2.7 μm
ポアサイズ
90 Å
動作pH範囲
2-9
アプリケーション
food and beverages
分離法
reversed phase
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関連するカテゴリー
詳細
アセンティス・エクスプレスHPLC カラムは、Fused-Core（フーズドコア）®粒子技術を用いることで、低い背圧を維持しながらサブ2 µm粒子の高速と高効率の両方を提供することができます。高い効率と低い背圧の組み合わせによって、従来のHPLC のユーザーだけではなくUPLC ®（または他の超高圧システム）のユーザーにも利益がもたらされます。
この新しいカラム技術のより詳細な情報については アセンティス・エクスプレスのホームページをご覧ください。
この新しいカラム技術のより詳細な情報については アセンティス・エクスプレスのホームページをご覧ください。
法的情報
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
UPLC is a registered trademark of Waters
ガードカートリッジ
必要ですが提供されていません
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
最新バージョンのいずれかを選択してください：
試験成績書（COA）
Lot/Batch Number
Liquid chromatography-mass spectrometry metabolite library for metabolomics: Evaluating column suitability using a scoring approach
Diamantidou D, et al.
Journal of Chromatography A, 1690, 463779-463779 (2023)
Dimitra Diamantidou et al.
Journal of chromatography. A, 1690, 463779-463779 (2023-01-22)
Untargeted metabolomic studies require an extensive set of analyte (metabolic) information to be obtained from each analyzed sample. Thus, highly selective, and efficient analytical methodologies together with reversed-phase (RP) or hydrophilic interaction liquid chromatography (HILIC) are usually applied in these
Ugandar Reddy Inugala et al.
Journal of chromatographic science, 51(5), 453-459 (2012-10-13)
This paper describes the development of a rapid, novel, stability-indicating gradient reversed-phase high-performance liquid chromatographic method and associated system suitability parameters for the analysis of naproxcinod in the presence of its related substances and degradents using a quality-by-design approach. All
Sreenivasa Rao Chitturi et al.
Journal of pharmaceutical and biomedical analysis, 55(1), 31-47 (2011-02-15)
This paper proposes a simple and selective RP-HPLC method for the determination of process impurities and degradation products (degradants) of atazanavir sulfate (ATV) drug substance. Chromatographic separation was achieved on Ascentis(®) Express C8, (150mm×4.6mm, 2.7μm) column thermostated at 30°C under
Luigi Silvestro et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 878(30), 3134-3142 (2010-10-20)
Quantitative methods using LC-MS/MS allow achievement of adequate sensitivity for pharmacokinetic studies with clopidogrel; three such methods, with LLOQs as low as 5 pg/mL, were developed and fully validated according to the well established FDA 2001 guidelines. The chromatographic separations
資料
Learn about Supel™ BioSPME C18 96-pin tool to prepare a clean sample with minimal matrix effects for an LC-MS/MS analysis from human plasma and its comparison with acetonitrile-based precipitation.
When a C18 doesn′t give the desired separation, or your sample contains compounds that are known to be difficult to retain or resolve on a C18, consider changing to an Ascentis® Express C8 column.
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Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?
1 回答-
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Can I use Ascentis Express on a UHPLC system?
1 回答-
Yes. Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
役に立ちましたか？
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Can I use Ascentis Express on any type of HPLC system?
1 回答-
Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems. Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
役に立ちましたか？
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Can Ascentis® Express HPLC Columns be used for LC-MS?
1 回答-
Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
役に立ちましたか？
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
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What flow rate should I use with Ascentis® Express HPLC Columns?
1 回答-
Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID; 0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
役に立ちましたか？
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How can I measure my instrument bandwidth (IBW) and determine what Ascentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?
1 回答-
The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
役に立ちましたか？
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Is there anything special I need to do to my HPLC system to use Ascentis Express?
1 回答-
Nothing special is required to use Ascentis Express HPLC columns. To obtain the full benefits of Ascentis Express, one should minimize dispersion in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102).
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