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Abdulameer E Al-Saffar et al.
Tropical animal health and production, 45(1), 327-334 (2012-07-31)
Two experiments were conducted to investigate the effect of phytase and/or multienzymes (protease, amyloglucoidase, xylanase, B-glucanase, cellulose, and hemicellulase) on improving the utilization of date pit (DP) in laying hens. In the first one, DP completely replaced corn in four...
Abdelbagi M Ismail et al.
Annals of botany, 103(2), 197-209 (2008-11-13)
Flooding slows seed germination, imposes fatalities and delays seedling establishment in direct-seeded rice. This study describes responses of contrasting rice genotypes subjected to flooding or low oxygen stress during germination and discusses the basis of tolerance shown by certain cultivars....
Wei Jiang Goh et al.
Langmuir : the ACS journal of surfaces and colloids, 28(49), 16864-16873 (2012-11-15)
Biofuels are fast advancing as a new research area to provide alternative sources of sustainable and clean energy. Recent advances in nanotechnology have sought to improve the efficiency of biofuel production, enhancing energy security. In this study, we have incorporated...
A blood trans-alpha-glucosylase.
Biochimica et biophysica acta, 22(1), 193-194 (1956-10-01)
Sachin Talekar et al.
Bioresource technology, 147, 269-275 (2013-09-04)
A tri-enzyme biocatalyst "combi-CLEAs" with starch hydrolytic activity was prepared from commercially available alpha amylase, glucoamylase and pullulanase preparations by aggregating enzymes with ammonium sulphate followed by cross-linking formed aggregates for 4.5h with 40 mM glutaraldehyde. The effects of precipitant...
Juan Antonio Tamayo-Ramos et al.
Biotechnology and bioengineering, 110(2), 543-551 (2012-09-06)
In filamentous fungi, most of the strategies used for the improvement of protein yields have been based on an increase in the transcript levels of a target gene. Strategies focusing at the translational level have been also described, but are...
Han-bing Zhang et al.
Zhonghua er ke za zhi = Chinese journal of pediatrics, 50(6), 415-419 (2012-08-31)
To analyze and summarize the characteristics of glycogen storage disease type II (Pompe disease) patients according to the clinical description and prognosis. Seventeen Chinese patients diagnosed by acid alpha-glucosidase (GAA) enzyme activity test were reviewed. Clinical data tables were designed....
The maltase of Clostridium acetobutylicum; its specificity range and mode of action.
D FRENCH et al.
The Journal of biological chemistry, 187(2), 463-471 (1950-12-01)
Swati B Jadhav et al.
Carbohydrate polymers, 98(1), 1191-1197 (2013-08-31)
Two enzymes, α-amylase and glucoamylase have been individually and co-conjugated to pectin by covalent binding. Both the enzyme systems showed better thermal and pH stability over the free enzyme system with the complete retention of original activities. Mixture of individually...
R.A. Rastall et al.
Biotechnology Letters, 13, 501-501 (1991)
Shay-Whey M Koh et al.
Journal of neurochemistry, 109(3), 792-806 (2009-03-03)
The neuropeptide vasoactive intestinal peptide (VIP) is anti-inflammatory and protective in the immune and nervous systems, respectively. This study demonstrated in corneal endothelial (CE) cells injured by severe oxidative stress (1.4 mM H(2)O(2)) in bovine corneal organ cultures that VIP...
Thomas Hundsberger et al.
Journal of neurology, 260(9), 2279-2285 (2013-06-12)
Glycogen storage disease type II is a rare multi-systemic disorder characterised by an intracellular accumulation of glycogen due a mutation in the acid alpha glucosidase (GAA) gene. The level of residual enzyme activity, the genotype and other yet unknown factors...
The subcellular distribution of enzymes in type II glycogenosis and the occurrence of an oligo-alpha-1,4-glucan glucohydrolase in human tissues.
B I Brown et al.
Biochimica et biophysica acta, 110(1), 124-133 (1965-10-25)
Ting-Ying Jiang et al.
PloS one, 7(7), e41131-e41131 (2012-07-21)
The N-terminal starch binding domain of Rhizopus oryzae glucoamylase (RoSBD) has a high binding affinity for raw starch. RoSBD has two ligand-binding sites, each containing a ligand-binding clamp: a polyN clamp residing near binding site I is unique in that...
Zaki A El-Fiky et al.
Journal of food science, 77(6), M312-M317 (2012-05-16)
Baker's yeast, Saccharomyces cerevisiae, is a key component in bread baking. Total of 12 commercial baker's yeast and 2 hybrid strains were compared using traditional quality parameters. Total of 5 strains with high leavening power and the 2 hybrid strains...
Tiziana Ventriglia et al.
Plant physiology, 148(1), 65-76 (2008-07-11)
ADP-glucose (Glc) pyrophosphorylase (ADP-Glc PPase) catalyzes the first committed step in starch biosynthesis. Higher plant ADP-Glc PPase is a heterotetramer (alpha(2)beta(2)) consisting of two small and two large subunits. There is increasing evidence that suggests that catalytic and regulatory properties...
Studies of lysosomal alpha-glucosidase. I. Purification and properties of the rat liver enzyme.
P L Jeffrey et al.
Biochemistry, 9(6), 1403-1415 (1970-03-17)
Properties of human intestinal glucoamylase.
J J Kelly et al.
Biochimica et biophysica acta, 315(1), 113-122 (1973-07-05)
M Kapelko et al.
Carbohydrate polymers, 97(2), 551-557 (2013-08-06)
The aim of the present study was to determine the impact of serial modifications of starch, including firstly starch extrusion or hydrolysis with pullulanase, followed by retrogradation (through freezing and defrosting of pastes) and acetylation (under industrial conditions), on its...
John A Maga et al.
The Journal of biological chemistry, 288(3), 1428-1438 (2012-11-29)
We have used a peptide-based targeting system to improve lysosomal delivery of acid α-glucosidase (GAA), the enzyme deficient in patients with Pompe disease. Human GAA was fused to the glycosylation-independent lysosomal targeting (GILT) tag, which contains a portion of insulin-like...
Xin Ren et al.
Food & function, 7(1), 372-379 (2015-10-28)
Foxtail millet, as a leading variety in arid and semi-arid areas of Asia and Africa, can provide broad potential benefits to human health. However, its digestion properties have not been reported. So in this study, the in vitro starch digestibilities...
Renata M Collares et al.
Journal of Zhejiang University. Science. B, 13(7), 579-586 (2012-07-05)
This work evaluates the enzymatic hydrolysis of starch from cassava using pectinase, α-amylase, and amyloglucosidase. A central composite rotational design (CCRD) was carried out to evaluate the effects of amyloglucosidase, pectinase, reaction time, and solid to liquid ratio. All the...
Abhishek Mukherjee et al.
Journal of enzyme inhibition and medicinal chemistry, 28(5), 900-910 (2012-07-19)
Azadirachta indica, used in antidiabetic herbal drugs, was reported to contain α-glucosidase inhibitor. Bioassay guided purification characterized the inhibitor as nimbidiol (a diterpenoid), present in root and stem-bark of the tree. Nimbidiol inhibited intestinal (mammalian) maltase-glucoamylase, sucrase-isomaltase, lactase, trehalase and...
Min Jin Kwon et al.
BMC genomics, 13, 701-701 (2012-12-15)
Filamentous fungi such as Aspergillus niger are well known for their exceptionally high capacity for secretion of proteins, organic acids, and secondary metabolites and they are therefore used in biotechnology as versatile microbial production platforms. However, system-wide insights into their...
Ji Sung Lim et al.
Journal of microbiology and biotechnology, 22(10), 1401-1405 (2012-10-19)
A thermotolerant Saccharomyces cerevisiae mutant strain, TT6, was constructed after multi-parental hybridization of five mutant strains obtained by UV or NTG treatment of the original strain, S. cerevisiae KV1. When incubated at 40 degrees C in YPD broth, TT6 began...
Preyesh Stephen et al.
PloS one, 7(11), e50488-e50488 (2012-12-12)
Glucoamylases, containing starch-binding domains (SBD), have a wide range of scientific and industrial applications. Random mutagenesis and DNA shuffling of the gene encoding a starch-binding domain have resulted in only minor improvements in the affinities of the corresponding protein to...
Ted J Langan et al.
Electrophoresis, 33(4), 607-613 (2012-03-28)
For the first time, a semi-permanent phospholipid coating is utilized in capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS). Although phospholipids in free solution are generally avoided in ESI, they did not interfere with the detection of linear and branched oligosaccharides using ESI...
Frank Martiniuk et al.
Applied biochemistry and biotechnology, 171(4), 916-926 (2013-08-03)
Genetic deficiency of acid alpha glucosidase (GAA) results in glycogen storage disease type II (GSDII) or Pompe's disease. To investigate whether we could generate a functional recombinant human GAA enzyme (tobrhGAA) in tobacco seeds for future enzyme replacement therapy, we...
Syun-ichi Yamakawa et al.
Enzyme and microbial technology, 50(6-7), 343-347 (2012-04-17)
A diploid yeast strain displaying both α-amylase and glucoamylase was developed for repeated fermentation from raw starch. First, the construct of α-amylase was optimized for cell surface display, as there have been no reports of α-amylase-displaying yeast. The modified yeast...
Cinthia Amiñoso et al.
Gene, 519(1), 169-172 (2013-02-14)
Pompe disease is an uncommon autosomal recessive glycogen storage disorder caused by deficiency of acid α-glucosidase. Classic infantile form triggers severe cardiomyopathy, hypotonia, and respiratory failure, leading to death within the first two years of life. The majority of patients...
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