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Nan Jiang et al.
Wei sheng wu xue bao = Acta microbiologica Sinica, 51(6), 725-731 (2011-08-27)
Due to its ability of hydrolyzing alpha-1,6-linkages, pullulanase plays a significant role in the industries where starch is used as raw material, including food processing and bioenergy. Exploitation of this enzyme focuses not only on screening novel strains, but also
Wei Yan et al.
Wei sheng wu xue bao = Acta microbiologica Sinica, 53(2), 145-153 (2013-05-01)
To increase extracellular productivityof pullulanase, pullulanase gene from Bacillus naganoensis JNB-1 was expressed in recombinant Escherichia coli, followed by optimizing induction conditions and applying chemical additives. We amplified pullulanase gene pul from B. naganoensis genome by PCR and constructed recombinant
Huanxin Zhang et al.
International journal of biological macromolecules, 52, 38-44 (2012-10-10)
The dissolution and digestion characteristics of maize starch processed with a combination of α-amylase and pullulanase (ERS) were investigated. The results were compared with those of high pressure-processed RS (HRS) and regular maize starch. The ERS exhibited a considerably lower
Yu-Liang Jiao et al.
Current microbiology, 62(1), 222-228 (2010-07-02)
The gene encoding a new extracellular amylopullulanase (type II pullulanase) was cloned from an extremely thermophilic anaerobic archaeon Thermococcus siculi strain HJ21 isolated previously from a deep-sea hydrothermal vent. The functional hydrolytic domain of the amylopullulanase (TsiApuN) and its MalE
Fu-Pang Lin et al.
Applied biochemistry and biotechnology, 165(3-4), 1047-1056 (2011-07-14)
The enzymatically active region of amylopullulanase from Thermoanaerobacterium saccharolyticum NTOU1 (TsaNTOU1Apu) was identified by truncation mutagenesis. Two truncated TsaNTOU1Apu enzymes, TsaNTOU1ApuM957 and TsaNTOU1ApuK885, were selected and characterized. Both TsaNTOU1ApuM957 and TsaNTOU1ApuK885 showed similar specific activities toward various substrates. The overall
Edward K Gilding et al.
Nature communications, 4, 1483-1483 (2013-02-14)
The production of adequate agricultural outputs to support the growing human population places great demands on agriculture, especially in light of ever-greater restrictions on input resources. Sorghum is a drought-adapted cereal capable of reliable production where other cereals fail, and
Marie-Noëlle Labour et al.
International journal of molecular sciences, 21(10) (2020-05-28)
Organoids production is a key tool for in vitro studies of physiopathological conditions, drug-induced toxicity assays, and for a potential use in regenerative medicine. Hence, it prompted studies on hepatic organoids and liver regeneration. Numerous attempts to produce hepatic constructs
S J King
Molecular oral microbiology, 25(1), 15-24 (2010-03-25)
Streptococcus pneumoniae colonization of the human airway is an essential precursor for disease; however, the mechanisms by which the bacterium establishes and maintains colonization are poorly understood. It is becoming increasingly clear that S. pneumoniae expresses glycosidases that can modify
Youran Li et al.
Journal of agricultural and food chemistry, 60(44), 11164-11172 (2012-10-18)
The pulA1 gene, encoding a novel thermostable type I pullulanase PulA1 from Bacillus sp. CICIM 263, was identified from genomic DNA. The open reading frame of the pulA1 gene was 2655 base pairs long and encoded a polypeptide (PulA1) of
Johanne Mørch Jensen et al.
FEBS letters, 586(16), 2479-2482 (2012-06-26)
Barley limit dextrinase (LD) that catalyses hydrolysis of α-1,6 glucosidic linkages in starch-derived dextrins is inhibited by limit dextrinase inhibitor (LDI) found in mature seeds. LDI belongs to the chloroform/methanol soluble protein family (CM-protein family) and has four disulfide bridges
T Han et al.
Letters in applied microbiology, 57(4), 336-343 (2013-06-25)
In this report, a glycoside hydrolase 13 family pullulanase gene (Tk0977) was cloned from a thermophilic anaerobic archaeon Thermococcus kodakarensis KOD1 (Pul-Tk). Pul-Tk encodes a protein of 765 amino acids including a putative 22-residue signal peptide. The protein has four
Joanna K Polko et al.
Current biology : CB, 28(19), 3174-3182 (2018-09-25)
Cell walls play critical roles in plants, regulating tissue mechanics, defining the extent and orientation of cell expansion, and providing a physical barrier against pathogen attack [1]. Cellulose microfibrils, which are synthesized by plasma membrane-localized cellulose synthase (CESA) complexes, are
Ghina Ali et al.
Carbohydrate polymers, 93(1), 109-115 (2013-03-08)
Pullulanase is an enzyme that hydrolyses the α-1,6 linkages of pullulan (Pull) to produce maltotriose units. We studied the capacity of pullulanase to cleave its modified substrate: carboxymethylpullulan (CMPull), synthesized with two different degrees of substitution (DS=0.16 and 0.8). Size
Fu-Pang Lin et al.
Extremophiles : life under extreme conditions, 16(3), 395-403 (2012-03-07)
The smallest and enzymatically active molecule, TetApuQ818, was localized within the C-terminal Q818 amino acid residue after serial C-terminal truncation analysis of the recombinant amylopullulanase molecule (TetApuM955) from Thermoanaerobacter pseudoethanolicus. Kinetic analyses indicated that the overall catalytic efficiency, k (cat)/K
Jinho Kang et al.
Enzyme and microbial technology, 48(3), 260-266 (2011-11-25)
The gene encoding a type I pullulanase from the hyperthermophilic anaerobic bacterium Thermotoga neapolitana (pulA) was cloned in Escherichia coli and sequenced. The pulA gene from T. neapolitana showed 91.5% pairwise amino acid identity with pulA from Thermotoga maritima and
Xiaoli Jin et al.
Biotechnology letters, 35(4), 639-645 (2012-12-25)
Limit dextrinase (LD) is a unique de-branching enzyme involved in starch mobilization of barley grains during malting, and closely related to malt quality. Genotypic variation of LD activity is controlled by genetic factors and also affected by environmental conditions. Correlation
Kornelia T Kaczmarska et al.
Journal of agricultural and food chemistry, 65(46), 10064-10073 (2017-10-24)
This study investigated the effect of germination and fermentation on the composition of carbohydrates in Australian sweet lupin. Specifically, the amount of sugars (sucrose, fructose, and glucose), starch, oligosaccharides (verbascose, stachyose, and raffinose), and dietary fiber were measured in germinated
Wen-Bo Chen et al.
Applied biochemistry and biotechnology, 169(1), 41-54 (2012-11-07)
A strain with the power to produce extracellular pullulanase was obtained from the sample taken from a flour mill. By sequencing its 16S rDNA, the isolate was identified as Klebsiella variicola SHN-1. When the gene encoding pullulanase, containing the N-terminal
Qingyan Wang et al.
International journal of systematic and evolutionary microbiology, 63(Pt 9), 3138-3142 (2013-02-26)
A novel α-amylase/pullulanase-producing bacterium, designated strain GST4(T), was isolated from samples collected from the wastewater of a cassava starch factory in Nanning, Guangxi Autonomous Region, southern China. Cells of strain GST4(T) were rod-shaped bacilli containing ellipsoidal terminal spores and found
Johanne Mørch Jensen et al.
Protein expression and purification, 79(2), 217-222 (2011-05-05)
The limit dextrinase inhibitor (LDI) from barley seeds acts specifically on limit dextrinase (LD), an endogenous starch debranching enzyme. LDI is a 14 kDa hydrophobic protein containing four disulfide bonds and one unpaired thiol group previously found to be either
Sachin Talekar et al.
Bioresource technology, 147, 269-275 (2013-09-04)
A tri-enzyme biocatalyst "combi-CLEAs" with starch hydrolytic activity was prepared from commercially available alpha amylase, glucoamylase and pullulanase preparations by aggregating enzymes with ammonium sulphate followed by cross-linking formed aggregates for 4.5h with 40 mM glutaraldehyde. The effects of precipitant
Haixia Li et al.
Plant cell reports, 30(9), 1641-1659 (2011-05-11)
High temperature has adverse effects on rice yield and quality. The different influences of night high temperature (NHT) and day high temperature (DHT) on rice quality and seed protein accumulation profiles during grain filling in indica rice '9311' were studied
Grzegorz Kłosowski et al.
Journal of bioscience and bioengineering, 109(5), 466-471 (2010-03-30)
The aim of the research was to assess the possibility of the fermentation productivity rising through the increase in corn mashes extract from 16-17 to 20-21 degrees Balling, yet keeping a 3-day fermentation period. The second goal was to obtain
Guodong Liu et al.
Carbohydrate polymers, 98(1), 995-1001 (2013-08-31)
This study aims to investigate the physicochemical and extended-release properties of amorphous debranched starch (ADBS) that is a linear short chain amylose derived from pullulanase enzymatic modification. The results show the completely amorphous ADBS was soluble in cold water, which
Bo Yu et al.
Molecules (Basel, Switzerland), 16(4), 3010-3017 (2011-04-09)
The effects of cyclodextrins and derivatives on the activity and structure of pullulanase were investigated in this study. Our results showed that cyclodextrins and derivatives decreased the activity of pullulanase. This decrease was attributed to the interaction between the hydrophobic
Adam Waśko et al.
Preparative biochemistry & biotechnology, 41(3), 252-261 (2011-06-11)
This paper describes a simple and efficient method of isolation of a plullulanase type I from amylolytic lactic acid bacteria (ALAB). Extracellular pullulanase type I was purified from a cell-free culture supernatant of Lactococcus lactis IBB 500 by using ammonium
Marie Sofie Møller et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 68(Pt 9), 1008-1012 (2012-09-06)
Barley limit dextrinase (HvLD) is a debranching enzyme from glycoside hydrolase family 13 subfamily 13 (GH13_13) that hydrolyses α-1,6-glucosidic linkages in limit dextrins derived from amylopectin. The structure of HvLD was solved and refined to 1.9 Å resolution. The structure
Malene Bech Vester-Christensen et al.
Journal of molecular biology, 403(5), 739-750 (2010-09-25)
Barley limit dextrinase [Hordeum vulgare limit dextrinase (HvLD)] catalyzes the hydrolysis of α-1,6 glucosidic linkages in limit dextrins. This activity plays a role in starch degradation during germination and presumably in starch biosynthesis during grain filling. The crystal structures of
Maria Laura Ferrando et al.
Microbiology (Reading, England), 156(Pt 9), 2818-2828 (2010-06-05)
We have identified apuA in Streptococcus suis, which encodes a bifunctional amylopullulanase with conserved alpha-amylase and pullulanase substrate-binding domains and catalytic motifs. ApuA exhibited properties typical of a Gram-positive surface protein, with a putative signal sequence and LPKTGE cell-wall-anchoring motif.
M Kapelko et al.
Carbohydrate polymers, 97(2), 551-557 (2013-08-06)
The aim of the present study was to determine the impact of serial modifications of starch, including firstly starch extrusion or hydrolysis with pullulanase, followed by retrogradation (through freezing and defrosting of pastes) and acetylation (under industrial conditions), on its
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