Cloning and Expression

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このクローニングプロトコルには、クローニングシステムおよびプラスミドベクターの選択、プラスミドの制限酵素消化、フラグメントの制限酵素消化、ゲル抽出、DNA脱リン酸化その他が含まれています。

Transformation is the process by which exogenous DNA is introduced into a cell, resulting in a heritable change or genetic modification. This was first reported in Streptococcus pneumoniae by Griffith in 1928. Transforming principle of DNA was demonstrated by Avery

Mgat4 May Play a Role in Increased Sialylation by Overexpressing Functional MGAT1 in Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells

MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.

Troubleshooting for Molecular Cloning

Molecular cloning is the process of inserting the gene-of-interest (GOI) into a plasmid vector and this vector is then inserted into a cell that expresses the protein encoded by the GOI. Once protein is expressed in the cell, the protein

Nucleic Acid Modifying Enzyme Selection Chart (Ultra Pure)

A helpful chart for selection of correct nucleic acid modifying enzyme.

トランスフェクションのしくみ

このウェビナーでは、トランスフェクションの概要および真核細胞にDNAやRNAを導入する方法について説明します。

コロニー選択のための青／白スクリーニングとそのプロトコル

さらなる解析のために、遺伝子組換え細菌コロニーを同定する分子生物学研究で用いられる青／白スクリーニング技法のプロトコルについて説明します。

FLAG®タグ細菌／哺乳類発現ベクター

細菌および哺乳類のタンパク質発現、検出、および精製に使用されるFLAG®および3xFLAG™発現ベクターならびに生成物。

制限エンドヌクレアーゼ - 分子のハサミ

「制限酵素」という用語は、ヴェルナー・アーバーとマシュー・メセルソンの研究所で行われた腸内細菌ファージλ（ラムダファージ）の研究に由来しています。

Cloning & Expression Vectors

Expression Vectors for Bacterial, Mammalian and Insect Cell Systems. Featured are a variety of tags, promoters and elements for secretion, transient, stable and bicistronic expression.

SnapFast™ Restriction Site Functions

Learn more about relevant restriction site functions in the SnapFast™ plasmid system. All DNA sections are pre-screened, and where possible modified, to remove any of the restriction sites found within the core SnapFast plasmids to maintain their flexibility.

T7 Promoter System

Bacterial Expression Vectors: T7 Promoter System. T7 Vectors for Highest Expression Levels in Bacteria.

Transforming E.coli with Engineered Plasmid

Making Competent Cells; Making Agar Plates; Bacterial Transformation; Picking Colonies; Growing Bacteria in Liquid Culture; Freezing Bacteria.

Restriction Enzyme Cloning Glossary

Plasmid Product Nomenclature

The SnapFast system is a versatile plasmid cloning platform that provides a range of functional DNA sequences in an easy to clone format. Hundreds of pre-designed DNA sections that can be easily incorporated into, or transferred between, our range of

Genotypes, Phenotypes and Markers

A genotype is a list of mutant genes in an organism. In addition to mutations of the genome, other genetic elements such as prophage or plasmids can also be included.

組換えタンパク質の超高感度検出を可能にする3xFLAG®システム発現ベクター

3xFLAGシステムは、3つのタンデムFLAG®エピトープを合計22個のアミノ酸に融合させた改良システムです。3xFLAGを含む融合タンパク質の検出感度は、他のシステムを最大で200倍上回ります。

細菌形質転換プロトコル

コンピテントセルの増殖とその形質転換（DNAの取込み）の一般的なプロトコル。

細胞へのトランスフェクション入門

トランスフェクションは、真核細胞にDNA、RNAまたはタンパク質を導入することであり、遺伝子発現の調節や研究に広く用いられています。トランスフェクション技術は、遺伝子機能、タンパク質合成、細胞増殖や発生の解析を容易にする分析ツールとして役立ちます。

Troubleshooting in pGEX Expression Vectors

This page describes troubleshooting strategies for cloning the gene or gene fragment into a pGEX expression vector.

Mgat4 May Play a Role in Increased Sialylation by Overexpressing Functional MGAT1 in Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells

MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.

T7 Promoter System

Bacterial Expression Vectors: T7 Promoter System. T7 Vectors for Highest Expression Levels in Bacteria.

Restriction Endonucleases - The Molecular Scissors

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.

Restriction Enzyme Cloning Glossary

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

Cloning Genes-of-Interest into a Plasmid Vector

This cloning protocol includes selecting the cloning system and plasmid vector, plasmid restriction digestion, fragment restriction digestion, gel excision, dephosphorylating DNA and more.

Simplicon™ in vivo Protein Expression System

Simplicon: A non-genome integrating, tunable and sustained protein expression system in human cells, based on a single, polycistronic, and self-replicating synthetic RNA.

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

Yeast Transformation Introduction

Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.

Introduction to Cell Transfection

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

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