Functional genomics screening
適用されているフィルター
アプリケーション:Functional genomics screening
コンテンツの種類:Technical Article
表示中 1-25 of 25 結果
Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways
Presenting an article on Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways
Discovery at Single-Cell Resolution
CRISPR lentiviral screening libraries are powerful research tools. Continuing a decade long commitment to library screening manufacturing, we have partnered with 10x Genomics to bring 10x Feature Barcode-compatible pooled screening products to you.
MISSION™ shRNA Library: Next Generation RNA Interference
MISSION™ shRNA Library: Next Generation RNA Interference
Methods for Enhancing Lentiviral Transduction Efficiency
An experiment to directly compare three methods of lentiviral transduction of Jurkat cells was conducted in order to determine the method that yields the greatest transduction efficiency.
CRISPRおよびRNAiスクリーニング実験を計画するための研究者向けガイド
このスクリーニングガイドには、細胞株、スクリーニングライブラリ、および実験条件の選択方法と、実験を計画および実行するためのヒントが記載されています。
Experimental Design and Analysis — Experimental Design FAQs
We answer your frequently asked questions about designing shRNA experiments, including appropriate controls, screening techniques, selection and more.
esiRNA よくあるお問い合わせ（FAQ）
カスタムおよびプレデザインesiRNA よくあるお問い合わせ（FAQ）
ｍiRNA（microRNA）の導入
成熟microRNA（miRNA）は天然に見られる非コードRNA低分子で、ヌクレオチド数は約21～25です。microRNAは1つ以上のメッセンジャーRNA（mRNA）分子と部分的相補性があり、その主な機能は翻訳抑制、mRNA切断、脱アデニル化など、さまざまな形式で遺伝子発現を低下させることです。
siRNA Delivery in Mammalian Cell Lines
siRNAs are effectively delivered into mammalian cell lines that have been difficult or even impossible to transfect with siRNA when using other commercially available lipofection reagents.
プレデザインsiRNA
ハイレベルなRNAi研究のため、メルクはバイオインフォマティクスのリーダーとして知られるRosetta Inpharmatics社と提携しています。Rosetta Inpharmatics社から導入し、メルクが独占的権利を有するsiRNA設計アルゴリズムは、RNAi用に性能をアップさせるため3年以上開発を続け、最適化を図ってきました。
Examination of Efficacy, Specificity, and Efficiency of an shRNA Lentiviral Library
The introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a ubiquitous tool in molecular biology.
Safe Lentivirus Handling
Our lentiviral vector systems are developed with enhanced safety features. Numerous precautions are in place in the design of our lentiviruses to prevent replication. Good handling practices are a must.
Examination of Efficacy, Specificity, and Efficiency of an shRNA Lentiviral Library
The introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a ubiquitous tool in molecular biology.
Safe Lentivirus Handling
Our lentiviral vector systems are developed with enhanced safety features. Numerous precautions are in place in the design of our lentiviruses to prevent replication. Good handling practices are a must.
MISSION shRNA Library: Next Generation RNA Interference
Work and refinement of the RNAi technology has exploded in recent years. We now know the basic mechanism of the endogenous RNAi pathway, that the pathway is present in most eukaryotes,3 and how cellular machinery can be harnessed to silence
Delivery of Nucleic Acids Using Polymers
This review will focus on the sub-sector of drug delivery concerning the delivery of nucleic acids, with a particular focus on poly(ethyleneimine).
miRNA (microRNA) Introduction
Mature microRNAs (miRNAs) are a class of naturally occurring, small non-coding RNA molecules, about 21-25 nucleotides in length. MicroRNAs are partially complementary to one or more messenger RNA (mRNA) molecules, and their main function is to downregulate gene expression
Nuclease-Free Water at Your Fingertips
An ultrafiltration cartridge can be placed at the outlet of a water purification system to deliver nuclease-free ultrapure water.
Experimental Design and Analysis — Experimental Design FAQs
We answer your frequently asked questions about designing shRNA experiments, including appropriate controls, screening techniques, selection and more.
Predesigned siRNA
We offer advanced design and quality synthesis for predesigned siRNA and siRNA libraries using the Rosetta siRNA Design Algorithm to reduce off-target effects and increase RNAi performance. The algorithm utilizes Position-Specific Scoring Matrices (PSSM) and knowledge of the siRNA seed
The Researcher’s Guide to Designing CRISPR and RNAi Screening Experiments
This screening guide covers how to choose a cell line, a screening library, and experimental conditions as well as tips for designing and performing your experiment.
Discovery at Single-Cell Resolution
CRISPR lentiviral screening libraries are powerful research tools. Continuing a decade long commitment to library screening manufacturing, we have partnered with 10x Genomics to bring 10x Feature Barcode-compatible pooled screening products to you.
esiRNA FAQs (Frequently Asked Questions)
esiRNA are endoribonuclease prepared siRNAs that target the same mRNA sequence for gene silencing. Here are some of the most asked questions regarding esiRNA uses and availability.
siRNA Delivery in Mammalian Cell Lines
siRNAs are effectively delivered into mammalian cell lines that have been difficult or even impossible to transfect with siRNA when using other commercially available lipofection reagents.
MISSION® siRNA FAQs
We answer your frequently asked questions about siRNA including experimental design and analysis, advanced applications, custom options and much more.