Gene expression and silencing

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facet applications:Gene expression and silencing

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Using Probe-Based Quantitative PCR (Qpcr) to Measure Gene-Level Expression

Quantitative PCR (qPCR) provides information about gene expression, gene amplification or loss, and small alterations. qPCR is often used to investigate tumor biology and to discover the genetic and epigenetic causes of cancer

Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways

Presenting an article on Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways

Efficient Gene Silencing using MISSION® TRC shRNA Lentiviral Vectors

The RNAi Consortium (TRC) is a collaborative effort among several academic laboratories (including the Broad Institute, the Whitehead Institute for Biomedical Research, the Dana Farber Cancer Institute, Massachusetts General Hospital, Massachusetts Institute of Technology, Harvard Medical School, Columbia University, and

MISSION® Lentiviral shRNA

MISSION shRNA reduce the expression of specific target genes by targeting the specific mRNA therefore reducing the corresponding protein expression.

pLKO-1 Vector & shRNA Design

Frequently asked questions about pLKO-1 vector & shRNA design

Using Microarray Technology to Select Housekeeping Genes in Chinese Hamster Ovary Cells

In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.

Lentiviral Transduction

In this exercise we will be demonstrating lentiviral transduction on two different cell lines.

siRNA Design, Manufacturing, Transfection & Detection

RNA interference (RNAi) is a natural biological mechanism wherein short inhibitory RNA (siRNA) duplexes induce potent inhibition of gene expression.

Phosphorothioate Oligonucleotides

Several phosphate backbone variants have been developed in an attempt to alter the chemical properties of native-state DNA and therefore overcome the two major challenges involved with using oligonucleotides in vivo.

Lentivirus Cell Line

Using lentivirus as a means to deliver shRNAs has become standard practice in many labs exploring RNAi.

Successful Transduction Using Lentivirus

You are not alone designing successful CRISPR, RNAi, and ORF experiments. Sigma-Aldrich was the first company to commercially offer lentivirus versions of targeted genome modification technologies and has the expertise and commitment to support new generations of scientists.

質量分析によるオリゴヌクレオチドの品質管理

質量分析は、オリゴヌクレオチド合成の分析に最適な技術です。性能に影響を及ぼす可能性のある低濃度の副産物を、最も高感度で検出することができます。

Technical Bulletins

Technical bulletins for our shRNA products, including Bacterial Glycerol Stock, Purified Plasmid DNA Format, Lentiviral Format, MISSION® Control Vectors, MISSION Control Transduction Particles, MISSION Lentiviral Packaging Mix.

MISSION™ shRNA Library: Next Generation RNA Interference

Development of a New Advanced KSOM Mouse Embryo Media that Enables Dual-Use Embryo Culture and Cell Handling Applications

Advanced KSOM mouse embryo media that can be used as a single medium for both harvest and culture of mouse embryos to facilitate creation of transgenic knockout mice.

逆転写

遺伝子発現解析へのアプローチの一つは、遺伝子のmRNA濃度の測定です。この解析には、異なる転写物間の半減期の差、転写物の時間的なパターン、mRNAとタンパク質との間の相関の欠落など、いくつもの課題があります。

General Adherent Cells

Protocol for creating create stable cell lines from both dividing and non-dividing cells for use with a lentiviral delivery system.

クロマチン免疫沈降（ChIP）法

クロマチン免疫沈降法（ChIP）を用いて、in vivoの単一部位または複数部位での特異的なタンパク質-DNA相互作用やタンパク質-タンパク質相互作用を検出し、相対的に定量することができます。

MISSION™ siRNA　よくあるお問い合わせ（FAQ）

こちらはsiRNAに関する実験計画や分析、高度なアプリケーション、カスタムオプションなどを含む、さまざまなよくあるお問い合わせに対する回答です。

一般的なshRNA　よくあるお問い合わせ（FAQ）

shRNA、TRCに関するよくあるお問い合わせにお答えします。MISSION™など。

MISSION^™ shRNAのご紹介

RNA干渉を用いた遺伝子サイレンシングおよびノックダウンはとても一般的になりつつあります。低分子干渉RNA（siRNA）の培養細胞への導入は、迅速かつ効率的に遺伝子発現をノックダウンする手段であるため、今やsiRNAは分子生物学分野の汎用ツールとなっています。

モレキュラービーコン

遺伝子研究および診断におけるモレキュラービーコンのパワーをご覧ください。In vitro遺伝子発現解析、遺伝子型決定など、これらのプローブをqPCRにおける特定の核酸配列のリアルタイム検出・モニタリングに使用する方法について説明します。

両末端標識プローブ

両末端標識プローブは、qPCRにおいて最も一般的なプローブで、加水分解プローブとも呼ばれます。

MISSION shRNA Lentiviral Transduction

MISSION® shRNA Lentiviral Transduction application data

Analysis of Fluorescent Dyes Used for Labeling DNA in Microarray Experiments

An article concerning Analyzing Properties of Fluorescent Dyes for Labeling DNA in Microarray Experiments presented by Sigma-Aldrich.com

CRISPR-based Gene Activation

Sigma has developed a gene activation system based on a fusion of dCas9 to the catalytic histone acetyltransferase (HAT) core domain of the human E1A-associated protein p300.

Expression of the Multiple Sclerosis-Associated MHC Class II Allele HLA-DRB1*1501 Is Regulated by Vitamin D

Ago RIP to Isolate microRNA and their Targets Using Imprint® RNA Immunoprecipitation Kit

Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.

Experimental Design and Analysis — Experimental Design FAQs

We answer your frequently asked questions about designing shRNA experiments, including appropriate controls, screening techniques, selection and more.

MISSION® shRNA Lentiviral Vectors Summary

RNA interference technology has been one of the key biological breakthroughs of the last decade and has revolutionized basic biology and gene function studies, but it also holds promise to revolutionize drug discovery and therapeutics.

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