PCR

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FastStart™ Taq DNAポリメラーゼ、5 U/µLのプロトコルとトラブルシューティング

PCRの結果は、PCR酵素と適切なバッファーの組み合わせの選択で大きく変わる可能性があります。

ThermaGenix Product Overview

How to stop nonspecific bands and off target primers in PCR.

Quantitative PCR and Digital PCR Detection Methods

Fluorescent dyes or probes are included in PCR mixes to monitor the change in NA amplicon concentration as the reaction proceeds.

Polymerase Chain Reaction

The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension

Long and Accurate PCR

Long and accurate PCR applications address the needs for longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase.

RT-PCR/RT-qPCRトラブルシューティング

データの信頼性確保には、PCR/RT-PCR/RT-qPCRトラブルシューティングプロトコルの作成が重要です。RT-PCRまたはPCRのエラーや問題の潜在的原因には、オペレーターのミス、PCRマスターミックス、オリゴデザインなどがあります。本PCRトラブルシューティングガイドでは、RT-PCRアッセイの概要と詳細な修正点について説明します。

Restorase® DNA Polymerase

Restorase® was developed for researchers unable to achieve amplification of damaged DNA templates when using other commercially available DNA polymerases.

KAPA Express Extract Kit FAQs

Frequently asked questions (FAQs) for KAPA Express Extract Kit.

Qualitative multiplex PCR assay for assessing DNA quality from FFPE tissues and other sources of damaged DNA

The assessment of DNA quality is a crucial first step in acquiring meaningful data from formalin-fixed paraffin-embedded (FFPE) tissues, and other sources of damaged DNA. Using intact genomic DNA is key for successful analysis of chromosomal aberrations (e.g. SNP analysis

Optimizing Crude Sample Plant PCR

Overcoming potent inhibitors and sample diversity in direct plant PCR.

PCR Troubleshooting Tips

When developing a PCR troubleshooting protocol, it is important to be open to any possible sources of error, however insignificant they may seem, in order to explore each potential problem independently.

FastStart™ Taq DNAポリメラーゼ、dNTPackのプロトコルとトラブルシューティング

PCRの結果は、PCR酵素と適切なバッファーの組み合わせを選択するかどうかで大きく左右される可能性があります。また、テンプレートの純度と品質もPCRを成功させる上で重要であり、配列やプライマー濃度も全体的なアッセイのクオリティーを決定します。ヌクレオチドは増幅反応に不可欠な成分であり、この試薬の純度と濃度はPCRの結果に大きな影響を及ぼします。

Introduction and Historical Timelines

Learn about the history of the polymerase chain reaction (PCR), from the basic principles that proceeded its discovery to the awarding of a Nobel Prize for Chemistry and more recent developments such as real-time PCR (qPCR) and digital PCR.

Whole Genome Amplification for Single Cell Biology

Whole genome amplification (WGA) offers a means to overcome the above restrictions for single-cell genomic analyses. WGA has been described as a non-specific amplification technique that affords an amplified product completely representative of the initial starting material.

PCR Assay Optimization and Validation

PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.

Extract-N-Amp™ Plant Tissue PCR Kits

The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that

Extract-N-Amp Reagent Provides a Highly Effective Way of Generating PCR Products from FTA® Blood Cards

Extract-N-Amp is a versatile, combined DNA extraction and amplification kit intended to simplify the generation of PCR products from a range of sample types. This methodology can be used without modification to perform PCR on blood samples stored on FTA

ThermaStop^™-RT PCR Additive

PCR additive interacts with reverse transcriptase at low temperatures to reduce priming problems that lead to nonspecific bands.

Technical Guide to PCR Technologies

Examples of basic PCR/qPCR/dPCR protocols that can be used as the foundation for explorations into some of the concepts described in the theoretical chapters of this guide. Included are detailed protocols for assay quality control, in addition to more general

質量分析によるオリゴヌクレオチドの品質管理

質量分析は、オリゴヌクレオチド合成の分析に最適な技術です。性能に影響を及ぼす可能性のある低濃度の副産物を、最も高感度で検出することができます。

Troubleshooting for Molecular Cloning

Molecular cloning is the process of inserting the gene-of-interest (GOI) into a plasmid vector and this vector is then inserted into a cell that expresses the protein encoded by the GOI. Once protein is expressed in the cell, the protein

Roche社のPCR試薬・PCRプロトコル

Taq DNAポリメラーゼのエラー率を低下させるRoche社のポリメラーゼ連鎖反応（PCR）試薬およびPCRセレクションガイド。

オリゴヌクレオチドの定量化

DNAは、その優れた結合特性により、UV分光計で容易に定量できます。

PCRの実践

一般的な考慮事項；RT-PCRに特異的な事項；キャリーオーバーコンタミネーションの防止；トラブルシューティング

PCR/qPCR/dPCRアッセイデザイン

ばらつきをもたらすさまざまな要因は、PCRのワークフロー全体に影響を及ぼします。サンプルの原料や逆転写ステップの要件など、変動要素の多くは避けることができないものです。アッセイデザインも変動性が高く、PCRの成否を分けうる要因であると同時に、アッセイの再現性や感度に貢献する要因でもあります。

GCリッチPCRシステムのトラブルシューティング

GCリッチ増幅ポリメラーゼ連鎖反応（PCR）システムのトラブルシューティング。

Whole Transcriptome Amplification of RNA from Low Cell-Number Samples

The efficacy of amplification of small quantities of total RNA with the Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study.

KAPA3G Plant PCR Kits FAQs

Frequently asked questions (FAQs) for KAPA3G Plant PCR Kits.

Extract-N-Amp™ Blood PCR Kit Protocol

The Extract-N-Amp Blood PCR Kits contain all the reagents needed to rapidly extract and amplify human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.

Oligonucleotide Array CGH Analysis of a Robust Whole Genome Amplification Method

In recent years, array-based Comparative Genomic Hybridization (aCGH) has been refined to determine chromosomal changes at progressively higher resolutions. This evolving technology is, however, somewhat hampered by the large DNA input requirement—a minimum of 150,000 copies of a human genome

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