Protein expression

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Applications:Protein expression

Content Type:Protocol

Showing 1-25 of 25 results

Duolink^® In Situ 簡易プロトコル – 蛍光顕微鏡観察用

このページでは、蛍光顕微鏡観察のためのDuolink® In Situの簡易プロトコルを紹介しています。

Duolink® PLA明視野検出プロトコル

このプロトコルでは、組織サンプルと細胞サンプルにおける個々のタンパク質、タンパク質修飾、およびタンパク質相互作用の明視野検出、視覚化、ならびに定量化のためのDuolink® PLA試薬の使用について説明します。

Duolink® PLA Multicolor検出プロトコル

タンパク質相互作用研究におけるDuolink^®の使用

このビデオでは、タンパク質相互作用やその修飾を1回の実験で検出、定量、細胞局在性を得ることができるシンプルでわかりやすい手順を紹介しています。

酵母形質転換のプロトコル

酵母は成長が速く、細胞が分散しているため、真核生物の研究に適したモデル生物と考えられています。

Liberase™ TM Research Grade Protocol & Troubleshooting

Duolink® PLA Probemaker Guide

Guide to create custom PLA probes for immunofluorescent or brightfield detection.

Counterstaining after the Duolink In Situ Protocol

We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.

Duolink® PLA Brightfield Protocol

This protocol describes the use of Duolink® PLA reagents for the brightfield detection, visualization, and quantification of individual proteins, protein modifications, and protein interactions in tissue and cell samples.

Duolink® In Situ Short Instructions - Fluorescence

This page details the Duolink® In Situ Short Protocol for fluorescence detection

Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

Yale Immunofluorescence Protocol

Procedure for Fixation, Immunostaining, and Imaging in 384-well Plates.

Duolink® PLA Fluorescence Protocol

This protocol describes how to perform immunofluorescent detection of proteins in cells and tissue.

Cancer Research Techniques

By focusing on analysis techniques and protocols specific for Cancer Research, we are putting your research needs first. Protein Tyrosine Kinase and Phosphatase Expression Profiling in Cancer Research.

Duolink® PLA Multicolor Detection Protocol

Handling Inclusion Bodies in Recombinant Protein Expression

How to isolate proteins from inclusion bodies using Cytiva products.

cOmplete™ Protocol

It is possible dissolve 1 tablet in as little as 2 ml double distilled Water, results in a 25x stock solution, without difficulty.

AliCE® Cell-Free Protein Synthesis Kit Quick Start Guide

Follow these five quick steps to perform cell-free protein synthesis using the ALiCE® protein expression plant lysate kit.

ALiCE®Cell-Free Protein Synthesis System Protocol

Achieve high protein yields with the ALiCE® Cell-Free Protein Synthesis System that allows you to obtain “difficult to produce” proteins in a matter of hours instead of weeks.

Measuring Kinase Inhibitor Residence Times

Jump dilution protocol to determine the residence time of a drug (or drug candidate) during its interaction with a kinase using a fluorescence polarization assay based on the detection of ADP.

Next Generation Cell Free Protein Expression Kit (Wheat Germ) (CFPS700) PROTOCOL

Yeast Growth Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

Duolink® PLA Flow Cytometry Protocol

Protocol for use of Duolink® PLA reagents for the detection of individual proteins, protein modifications, and protein-protein interactions within cell populations by flow cytometry.

How to Create PLA® Probes

Duolink® kits use in situ PLA®, a proximity ligation assay technology, to accurately and objectively quantify individual proteins, and their interactions and modifications in unmodified cells and tissue.

Using Duolink® in Protein Interaction Studies

The video follows the simple and straightforward procedure that allows you to detect, quantify and obtain cell localization of protein interactions and their modifications in a single experiment.