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Protein quantitation

facet applications:Protein quantitation
facet content type:Protocol
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Duolink® In Situ 簡易プロトコル – 蛍光顕微鏡観察用
このページでは、蛍光顕微鏡観察のためのDuolink® In Situの簡易プロトコルを紹介しています。
Duolink® PLA明視野検出プロトコル
このプロトコルでは、組織サンプルと細胞サンプルにおける個々のタンパク質、タンパク質修飾、およびタンパク質相互作用の明視野検出、視覚化、ならびに定量化のためのDuolink® PLA試薬の使用について説明します。
Duolink® PLA Multicolor検出プロトコル
Duolink® PLA Multicolor検出プロトコル
LC-UV-MS Method Development for Antibody Drug Conjugates Using a Non-Toxic ADC-Mimic
SigmaMab Antibody Drug Conjugate Mimic, is a non-toxic drug mimic utilized as a standard for mass spectrometry and high performance liquid chromatography.
Duolink® PLA Probemaker Guide
Guide to create custom PLA probes for immunofluorescent or brightfield detection.
Counterstaining after the Duolink In Situ Protocol
We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.
Duolink® PLA Brightfield Protocol
This protocol describes the use of Duolink® PLA reagents for the brightfield detection, visualization, and quantification of individual proteins, protein modifications, and protein interactions in tissue and cell samples.
Duolink® In Situ Short Instructions - Fluorescence
This page details the Duolink® In Situ Short Protocol for fluorescence detection
Protein Determination by the Warburg-Christian Method
To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.
Protein Determination Modified Lowry Method
To standardize a procedure for the determination of protein by modified Lowry.
Duolink® PLA Multicolor Detection Protocol
Duolink® PLA Multicolor Detection Protocol
Production of 15N-13C Dual-labelled Proteins
Dr. Alan Robertson and Dr. Ben Davis at Vernalis, Ltd., UK, a provider of drug discovery and development services, have refined a novel protocol for the duallabelling of proteins using EnPresso® B Defined Nitrogen-free.
Duolink® PLA Flow Cytometry Protocol
Protocol for use of Duolink® PLA reagents for the detection of individual proteins, protein modifications, and protein-protein interactions within cell populations by flow cytometry.
How to Create PLA® Probes
Duolink® kits use in situ PLA®, a proximity ligation assay technology, to accurately and objectively quantify individual proteins, and their interactions and modifications in unmodified cells and tissue.
Using Duolink® in Protein Interaction Studies
The video follows the simple and straightforward procedure that allows you to detect, quantify and obtain cell localization of protein interactions and their modifications in a single experiment.
Protein Determination by the Bicinchoninic Acid (BCA) Method
A standard procedure for the quantitative determination of total protein concentration of a solution by the BCA method.
Protein Determination using 2-D Quant Kit
2-D Quant Kit from Cytiva is designed to accurately determine protein concentrations in samples for electrophoresis.