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Protein quantitation

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facet applications:Protein quantitation
facet content type:Technical Article
表示中 1-28 of 28 結果
フローサイトメトリーによる検出のためのDuolink® PLAを最適化する方法
フローサイトメトリープロトコルのための、適切な実験デザイン、Duolink® PLAの準備と実施する際の検討事項
フローサイトメトリー検出のためのDuolink® PLAトラブルシューティングガイド
フローサイトメトリーのためのDuolink® PLA実験で成果を上げるために、正しく実験を行うための一般的なヒントやコツ、問題を特定するための有用な情報と解決策を提供します。
ぺプチドの保管と取扱い
カスタムペプチドの保管および取扱い
Duolink® PLAフローサイトメトリーキット
従来のフローサイトメトリーでは、これまで、タンパク質間相互作用や微量タンパク質発現を検出する機能に制限がありました。そこでわたしたちは、Duolink®近接ライゲーションアッセイ(PLA)とフローサイトメトリーを組み合わせた便利なキットを作成し、フローサイトメトリーの測定値でタンパク質間相互作用と分析できるようにしました。
神経科学アプリケーション向けDuolink™ PLAテクノロジー
Duolink™近接ライゲーションアッセイ(PLA)テクノロジーは、神経細胞の相互作用を研究するために使用でき、神経科学の研究を促進します。
Duolink®近接ライゲーションアッセイ説明動画
この動画は、Duolink® PLA実験の方法について、ユーザーが固定化および膜透過処理をおこなった細胞を用いて手順を追って説明しています。
Duolink®近接ライゲーションアッセイを最適化する方法
Duolink®アッセイプロトコルの準備・セットアップ・実施にあたり考慮すべき事項
高感度多重検出のためのDuolink®近接ライゲーションアッセイ(PLA)マルチカラーキット
Duolink® PLA マルチカラーテクノロジーおよび固定した組織・細胞におけるタンパク質間相互作用の定量化。
Single Molecule Detection with Atto 647N NTA
Significant improvement of single molecule tracking by using superior fluorescent NTA-Atto 647N conjugate
Multiplex Quantification of Infliximab and Adalimumab in Human Serum by LC-MS/MS Using Full-Length Stable Isotope Labeled Internal Standards
Infliximab and Adalimumab monoclonal antibodies (mAbs) are used widely to treat rheumatoid arthritis, psoriatic arthritis, and many autoimmune diseases by binding to tumor necrosis factor-alpha (TNFα) to reduce the inflammatory response.
Quantification of Host Cell Proteins
Residual presence of host cell proteins (HCPs) in recombinant therapeutic products has considerable clinical safety risks associated with a potential immunological response in patients.
Absolute Quantification of Serum Proteome
Absolute Quantification (Protein-AQUA™™) is a targeted quantitative proteomics technique that exhibits robust efficacy and is being increasingly utilized for a wide variety of quantitative proteomics studies.
Detection of Protein-protein Interactions by in situ PLA
Proteins are complex biological molecules essential for cellular structure and functions. The majority of proteins commonly interact with various molecules, including other proteins, in order to exert their functions.
Duolink® Proximity Ligation Assay Instructional Video
This video will show you step-by-step how to perform a Duolink® PLA experiment with cells on a slide that have already been fixed and permeabilized by the user.
Duolink® Proximity Ligation Assay (PLA) Multicolor Kits for Sensitive Multiplex Detection
Duolink® PLA Multicolor technology and the quantification of protein-protein interactions in fixed tissues and cells.
Handling Storage
Handling & Storage of Custom Peptides
Peptide Modifications: N-Terminal, Internal, and C-Terminal
Peptide Modifications: N-Terminal, Internal, and C-Terminal
How to Optimize the Duolink® Proximity Ligation Assay
Things to consider for preparation, setup and execution of the Duolink® assay protocol
Troubleshooting 2-D DIGE Results
This troubleshooting table lists problems that may be encountered in two-dimensional difference gel electrophoresis (2D DIGE) results, including possible causes and methods to prevent future problems.
Mass Spectrometry
What is Mass Spectrometry or MS? What is it used for and how does it work? Read this detailed article with diagrams and example graphs to understand how to use mass spec in your research.
Quantification of Infliximab in Human Serum by LC-MS/MS Using A Full-Length Stable Isotope Labeled Internal Standard
Infliximab, a chimeric monoclonal antibody, is used to treat rheumatoid arthritis, psoriatic arthritis, Crohn’s disease, and other autoimmune diseases.
Studies of Dimerization and Activation of Receptors in the EGFR-Family Using Duolink® Assays
Our aim was to investigate the dimerization and activation pattern of two receptors, EGFR (ErbB1) and HER2 (ErbB2), in the EGFR-family using breast cancer cell lines and selected colon tissues as model systems.
How to Optimize Duolink® PLA for Flow Cytometry Detection
Considerations for proper experimental design, preparation and execution of the Duolink® PLA for flow cytometry protocol.
Duolink® PLA Technology for Neuroscience Applications
Duolink® proximity ligation assay used to study neuron interactions furthering neuroscience research.
Duolink® PLA Flow Cytometry Kits
Traditional flow cytometry has been limited in the ability to detect protein-protein interactions and low abundant proteins events — until now. We have combined Duolink® Proximity Ligation Assay (PLA) with flow cytometry in a convenient kit, making the analysis of
Peptide Sample Amount Determination
Determining the amount of desired product in a peptide sample.
Direct Detect® Infrared Spectrometer for Total Protein Quantitation
Learn how total protein quantitation with an infrared spectrometer may be a more accurate and universal alternative to Bradford, BCA, or UV-based methods.
Troubleshooting Guide for Duolink® PLA for Flow Cytometry Detection
General tips and tricks for proper experiment execution, aid in identifying potential problems, and provide solutions to ensure a successful Duolink® PLA experiment for flow cytometry.