Merck

Comparative lipid profile of four edible shellfishes by UPLC-Triple TOF-MS/MS.

Food chemistry (2019-12-17)
He Li, Yu Song, Hongwei Zhang, Xuesong Wang, Peixu Cong, Jie Xu, Changhu Xue
要旨

An ultra performance liquid chromatography-Triple time of flight mass spectrometry (UPLC-Triple TOF-MS/MS) method were established to characterize the lipid profiles in four shellfish species. More than 600 lipid molecular species belonging to 14 classes were detected. Phospholipids (PLs) were predominant in Chlamys farreri (54.9%) and glycerolipids (GLs) were dominant in Ostrea gigas (51.6%). PLs that contained polyunsaturated fatty acids (PUFAs) such as PC (16:0/20:5), PC (16:0/22:6) and PE (18:0/22:6) were the main molecular species. Especially, the percentage of sphingolipids (SLs) in four shellfishes is considerable (18.8-38.6%), the characterization of their special long-chain base (LCB) structure (mainly d19:3) and N-acyl group (mainly 16:0) was realized. Several SL subclasses with low abundance in four shellfish species, such as ceramide 2-aminoethylphosphonate (CAEP) and deoxy-ceramide (DeoxyCer), were also detected. These active lipids identified by this method have potential value in revealing the nutritional value of shellfishes and serving as biomarkers for distinguishing different shellfishes.

材料
製品番号
ブランド
製品内容

Avanti
14:0 PC (DMPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine, powder
Avanti
14:0 PG, Avanti Polar Lipids
Avanti
14:0 PC (DMPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine, chloroform
Avanti
14:0 PS, Avanti Polar Lipids
Avanti
C17 セラミド (d18:1/17:0), Avanti Polar Lipids 860517P, powder
Avanti
14:0 PE, Avanti Polar Lipids
Avanti
16:0 PI, 1,2-dipalmitoyl-sn-glycero-3-phospho-(1′-myo-inositol) (ammonium salt), powder
Avanti
12:0 SM (d18:1/12:0), Avanti Polar Lipids 860583P, powder
Avanti
C17 Mono-Sulfo Galactosyl(β) Ceramide (d18:1/17:0), Avanti Polar Lipids 860572P, powder
Avanti
14:0 Lyso PE, 1-myristoyl-2-hydroxy-sn-glycero-3-phosphoethanolamine, powder
Avanti
19:0 Lyso PC, Avanti Polar Lipids 855776P, powder
Avanti
19:0 Lyso PC, Avanti Polar Lipids 855776C