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Phosphoproteomics reveals conserved exercise-stimulated signaling and AMPK regulation of store-operated calcium entry.

The EMBO journal (2019-08-06)
Marin E Nelson, Benjamin L Parker, James G Burchfield, Nolan J Hoffman, Elise J Needham, Kristen C Cooke, Timur Naim, Lykke Sylow, Naomi Xy Ling, Deanne Francis, Dougall M Norris, Rima Chaudhuri, Jonathan S Oakhill, Erik A Richter, Gordon S Lynch, Jacqueline Stöckli, David E James
ABSTRACT

Exercise stimulates cellular and physiological adaptations that are associated with widespread health benefits. To uncover conserved protein phosphorylation events underlying this adaptive response, we performed mass spectrometry-based phosphoproteomic analyses of skeletal muscle from two widely used rodent models: treadmill running in mice and in situ muscle contraction in rats. We overlaid these phosphoproteomic signatures with cycling in humans to identify common cross-species phosphosite responses, as well as unique model-specific regulation. We identified > 22,000 phosphosites, revealing orthologous protein phosphorylation and overlapping signaling pathways regulated by exercise. This included two conserved phosphosites on stromal interaction molecule 1 (STIM1), which we validate as AMPK substrates. Furthermore, we demonstrate that AMPK-mediated phosphorylation of STIM1 negatively regulates store-operated calcium entry, and this is beneficial for exercise in Drosophila. This integrated cross-species resource of exercise-regulated signaling in human, mouse, and rat skeletal muscle has uncovered conserved networks and unraveled crosstalk between AMPK and intracellular calcium flux.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
タプシガルギン, ≥98% (HPLC), solid film
Sigma-Aldrich
AICAR, ≥98% (HPLC), powder
Sigma-Aldrich
水素化ホウ素テトラエチルアンモニウム, technical, ≥95% (T)