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  • The CHORD protein CHP-1 regulates EGF receptor trafficking and signaling in C. elegans and in human cells.

The CHORD protein CHP-1 regulates EGF receptor trafficking and signaling in C. elegans and in human cells.

eLife (2020-02-14)
Andrea Haag, Michael Walser, Adrian Henggeler, Alex Hajnal
ABSTRACT

The intracellular trafficking of growth factor receptors determines the activity of their downstream signaling pathways. Here, we show that the putative HSP-90 co-chaperone CHP-1 acts as a regulator of EGFR trafficking in C. elegans. Loss of chp-1 causes the retention of the EGFR in the ER and decreases MAPK signaling. CHP-1 is specifically required for EGFR trafficking, as the localization of other transmembrane receptors is unaltered in chp-1(lf) mutants, and the inhibition of hsp-90 or other co-chaperones does not affect EGFR localization. The role of the CHP-1 homolog CHORDC1 during EGFR trafficking is conserved in human cells. Analogous to C. elegans, the response of CHORDC1-deficient A431 cells to EGF stimulation is attenuated, the EGFR accumulates in the ER and ERK2 activity decreases. Although CHP-1 has been proposed to act as a co-chaperone for HSP90, our data indicate that CHP-1 plays an HSP90-independent function in controlling EGFR trafficking through the ER.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
hEGF, EGF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Sigma-Aldrich
SOLu-Trypsin, recombinant, expressed in Pichia pastoris, Proteomics Grade, liquid
Sigma-Aldrich
モノクロナール抗MAPキナーゼ, 活性化 (二リン酸化型ERK-1&2) マウス宿主抗体, clone MAPK-YT, ascites fluid
Sigma-Aldrich
抗MAPキナーゼ(ERK-1, ERK-2) ウサギ宿主抗体, whole antiserum
Sigma-Aldrich
抗CHORDC1抗体 ウサギ宿主抗体, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
抗CHORDC1抗体 ウサギ宿主抗体, affinity isolated antibody, buffered aqueous solution