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High-amylose wheat starch: Structural basis for water absorption and pasting properties.

Carbohydrate polymers (2020-07-29)
Caili Li, Sushil Dhital, Robert G Gilbert, Michael J Gidley
ABSTRACT

High-amylose wheat starch (HAWS) and flour (HAWF) have the potential to deliver food products with enhanced nutritional functionality, but structure/function relationships are not well understood. We report the structural bases for differences in water absorption and pasting properties for HAWS and HAWF (amylose contents 71-84 %) compared with wild-type (WTWS/WTWF). With higher amylose content, the proportion of longer amylopectin chains with DP > 25 increased. Both the degree of branching and the branch lengths of amylose were lower for HAWS than WTWS. Compared with WTWF, HAWF contained less total starch, more protein, had lower peak viscosity by high-temperature RVA, lower dough development time and stability time and higher water absorption by Farinograph. Water absorption by HAWS was ∼1.5 times greater than for WTWS, suggesting loose packing of polymers within HAWS granules. Consistent with this, crystallinity and birefringence of starch granules were lower in HAWS.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
アミロース ジャガイモ由来, used as amylase substrate
Sigma-Aldrich
アミロペクチン トウモロコシ由来