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  • The catalytic subunit of Plasmodium falciparum casein kinase 2 is essential for gametocytogenesis.

The catalytic subunit of Plasmodium falciparum casein kinase 2 is essential for gametocytogenesis.

Communications biology (2021-03-14)
Eva Hitz, Olivia Grüninger, Armin Passecker, Matthias Wyss, Christian Scheurer, Sergio Wittlin, Hans-Peter Beck, Nicolas M B Brancucci, Till S Voss
要旨

Casein kinase 2 (CK2) is a pleiotropic kinase phosphorylating substrates in different cellular compartments in eukaryotes. In the malaria parasite Plasmodium falciparum, PfCK2 is vital for asexual proliferation of blood-stage parasites. Here, we applied CRISPR/Cas9-based gene editing to investigate the function of the PfCK2α catalytic subunit in gametocytes, the sexual forms of the parasite that are essential for malaria transmission. We show that PfCK2α localizes to the nucleus and cytoplasm in asexual and sexual parasites alike. Conditional knockdown of PfCK2α expression prevented the transition of stage IV into transmission-competent stage V gametocytes, whereas the conditional knockout of pfck2a completely blocked gametocyte maturation already at an earlier stage of sexual differentiation. In summary, our results demonstrate that PfCK2α is not only essential for asexual but also sexual development of P. falciparum blood-stage parasites and encourage studies exploring PfCK2α as a potential target for dual-active antimalarial drugs.

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ブランド
製品内容

Sigma-Aldrich
クロロキン 二リン酸塩, powder or crystals, 98.5-101.0% (EP)
Roche
抗GFP, from mouse IgG1κ (clones 7.1 and 13.1)
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塩化コリン, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥98%
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キナリザリン, ≥95% (HPLC)
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TBB, ≥98% (HPLC), solid
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DMAT, ≥98% (HPLC)