Merck
  • Preparation of the membrane-permeant biarsenicals FlAsH-EDT2 and ReAsH-EDT2 for fluorescent labeling of tetracysteine-tagged proteins.

Preparation of the membrane-permeant biarsenicals FlAsH-EDT2 and ReAsH-EDT2 for fluorescent labeling of tetracysteine-tagged proteins.

Nature protocols (2008-09-06)
Stephen R Adams, Roger Y Tsien
要旨

The membrane-permeant fluorogenic biarsenicals FlAsH-EDT(2) and ReAsH-EDT(2) can be prepared in good yields by a straightforward two-step procedure from the inexpensive precursor dyes fluorescein and resorufin, respectively. Handling of toxic reagents such as arsenic trichloride is minimized so the synthesis can be carried out in a typical chemistry laboratory, usually taking about 2-3 d. A wide range of other biarsenical reagents and intermediates that also bind to tetracysteine-tagged (CysCysProGlyCysCys) proteins can be prepared similarly using this general procedure.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
1-メチル-2-ピロリジノン, anhydrous, 99.5%
Sigma-Aldrich
N,N-ジイソプロピルエチルアミン, purified by redistillation, 99.5%
Sigma-Aldrich
酢酸パラジウム(II), reagent grade, 98%
Supelco
2-メルカプトエタンスルホン酸ナトリウム, analytical standard, ≥98.0% (titration)
Sigma-Aldrich
1,2-エタンジチオール, technical grade, ≥90%
Sigma-Aldrich
Aldrich® Firestoneバルブ, controls gas flow and pressure in reaction vessels
Sigma-Aldrich
Palladium(II) Acetate ChemBeads