Merck
  • Home
  • Search Results
  • Platelet activation and apoptosis modulate monocyte inflammatory responses in dengue.

Platelet activation and apoptosis modulate monocyte inflammatory responses in dengue.

Journal of immunology (Baltimore, Md. : 1950) (2014-07-13)
Eugenio D Hottz, Isabel M Medeiros-de-Moraes, Adriana Vieira-de-Abreu, Edson F de Assis, Rogério Vals-de-Souza, Hugo C Castro-Faria-Neto, Andrew S Weyrich, Guy A Zimmerman, Fernando A Bozza, Patrícia T Bozza
ABSTRACT

Dengue is the most prevalent human arbovirus disease in the world. Dengue infection has a large spectrum of clinical manifestations, from self-limited febrile illness to severe syndromes accompanied by bleeding and shock. Thrombocytopenia and vascular leak with altered cytokine profiles in plasma are features of severe dengue. Although monocytes have been recognized as important sources of cytokines in dengue, the contributions of platelet-monocyte interactions to inflammatory responses in dengue have not been addressed. Patients with dengue were investigated for platelet-monocyte aggregate formation. Platelet-induced cytokine responses by monocytes and underlying mechanisms were also investigated in vitro. We observed increased levels of platelet-monocyte aggregates in blood samples from patients with dengue, especially patients with thrombocytopenia and increased vascular permeability. Moreover, the exposure of monocytes from healthy volunteers to platelets from patients with dengue induced the secretion of the cytokines IL-1β, IL-8, IL-10 and MCP-1, whereas exposure to platelets from healthy volunteers only induced the secretion of MCP-1. In addition to the well-established modulation of monocyte cytokine responses by activated platelets through P-selectin binding, we found that interaction of monocytes with apoptotic platelets mediate IL-10 secretion through phosphatidylserine recognition in platelet-monocyte aggregates. Moreover, IL-10 secretion required platelet-monocyte contact but not phagocytosis. Together, our results demonstrate that activated and apoptotic platelets aggregate with monocytes during dengue infection and signal specific cytokine responses that may contribute to the pathogenesis of dengue.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
HEPES, ≥99.5% (titration)
Sigma-Aldrich
塩化ナトリウム, BioXtra, ≥99.5% (AT)
Sigma-Aldrich
HEPES, BioPerformance Certified, ≥99.5% (titration), suitable for cell culture
Sigma-Aldrich
重炭酸ナトリウム, powder, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture
Sigma-Aldrich
重炭酸ナトリウム, ACS reagent, ≥99.7%
Sigma-Aldrich
塩化ナトリウム, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)
Sigma-Aldrich
塩化ナトリウム 溶液, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture
Sigma-Aldrich
塩化ナトリウム, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
Sigma-Aldrich
塩化ナトリウム 溶液, 0.9% in water, BioXtra, suitable for cell culture
Sigma-Aldrich
塩化ナトリウム 溶液, 5 M
Sigma-Aldrich
HEPES緩衝液, 1 M in H2O
Sigma-Aldrich
塩化ナトリウム, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%
Sigma-Aldrich
重炭酸ナトリウム, ReagentPlus®, ≥99.5%, powder
SAFC
HEPES
Sigma-Aldrich
重炭酸ナトリウム, BioXtra, 99.5-100.5%
Sigma-Aldrich
塩化ナトリウム, JIS special grade, ≥99.5%
Sigma-Aldrich
塩化ナトリウム, BioUltra, for molecular biology, ≥99.5% (AT)
Sigma-Aldrich
HEPES, BioUltra, for molecular biology, ≥99.5% (T)
Supelco
塩化ナトリウム, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
HEPES, BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)
Sigma-Aldrich
塩化ナトリウム 溶液, 0.85%
Sigma-Aldrich
塩化ナトリウム 溶液, BioUltra, for molecular biology, ~5 M in H2O
SAFC
HEPES
Supelco
塩化ナトリウム, certified reference material for titrimetry, certified by BAM, ≥99.5%
Sigma-Aldrich
重炭酸ナトリウム, anhydrous, free-flowing, Redi-Dri, ACS reagent, ≥99.7%
Sigma-Aldrich
塩化ナトリウム, 99.999% trace metals basis
Sigma-Aldrich
重炭酸ナトリウム, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, E500, 99.0-100.5%, powder
Sigma-Aldrich
HEPES, BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)
Sigma-Aldrich
塩化ナトリウム, BioPerformance Certified, ≥99% (titration), suitable for insect cell culture, suitable for plant cell culture
Sigma-Aldrich
塩化ナトリウム, SAJ first grade, ≥99.0%