Merck

Structural basis for self-assembly of a cytolytic pore lined by protein and lipid.

Nature communications (2015-02-27)
Koji Tanaka, Jose M M Caaveiro, Koldo Morante, Juan Manuel González-Mañas, Kouhei Tsumoto
要旨

Pore-forming toxins (PFT) are water-soluble proteins that possess the remarkable ability to self-assemble on the membrane of target cells, where they form pores causing cell damage. Here, we elucidate the mechanism of action of the haemolytic protein fragaceatoxin C (FraC), a α-barrel PFT, by determining the crystal structures of FraC at four different stages of the lytic mechanism, namely the water-soluble state, the monomeric lipid-bound form, an assembly intermediate and the fully assembled transmembrane pore. The structure of the transmembrane pore exhibits a unique architecture composed of both protein and lipids, with some of the lipids lining the pore wall, acting as assembly cofactors. The pore also exhibits lateral fenestrations that expose the hydrophobic core of the membrane to the aqueous environment. The incorporation of lipids from the target membrane within the structure of the pore provides a membrane-specific trigger for the activation of a haemolytic toxin.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
塩化ナトリウム, BioXtra, ≥99.5% (AT)
Sigma-Aldrich
塩化ナトリウム, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)
Sigma-Aldrich
塩化ナトリウム 溶液, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture
Sigma-Aldrich
塩化ナトリウム, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
Sigma-Aldrich
塩化ナトリウム 溶液, 0.9% in water, BioXtra, suitable for cell culture
Sigma-Aldrich
塩化ナトリウム 溶液, 5 M
Sigma-Aldrich
N,N-ジメチルドデシルアミン N-オキシド 溶液, ~30% in H2O
Sigma-Aldrich
塩化ナトリウム, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%
Sigma-Aldrich
塩化ナトリウム, JIS special grade, ≥99.5%
Sigma-Aldrich
塩化ナトリウム, BioUltra, for molecular biology, ≥99.5% (AT)
Supelco
塩化ナトリウム, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
塩化ナトリウム 溶液, 0.85%
Sigma-Aldrich
塩化ナトリウム 溶液, BioUltra, for molecular biology, ~5 M in H2O
Supelco
塩化ナトリウム, certified reference material for titrimetry, certified by BAM, ≥99.5%
Sigma-Aldrich
N,N-ジメチルドデシルアミンN-オキシド, BioXtra, ≥99.0% (NT)
Sigma-Aldrich
塩化ナトリウム, 99.999% trace metals basis
Sigma-Aldrich
塩化ナトリウム, BioPerformance Certified, ≥99% (titration), suitable for insect cell culture, suitable for plant cell culture
Sigma-Aldrich
塩化ナトリウム, SAJ first grade, ≥99.0%
Sigma-Aldrich
塩化ナトリウム, tested according to Ph. Eur.
Sigma-Aldrich
trans-デヒドロアンドロステロン, ≥99%
Sigma-Aldrich
塩化ナトリウム, tablet
Sigma-Aldrich
塩化ナトリウム, AnhydroBeads, −10 mesh, 99.999% trace metals basis
Sigma-Aldrich
8-アミノナフタレン-1,3,6-トリスルホン酸 二ナトリウム塩, BioReagent, suitable for fluorescence, ≥90% (CE)
Sigma-Aldrich
塩化ナトリウム 溶液, 1 M
Sigma-Aldrich
塩化ナトリウム, random crystals, optical grade, 99.9% trace metals basis
Sigma-Aldrich
塩化ナトリウム, AnhydroBeads, −10 mesh, 99.99% trace metals basis
Sigma-Aldrich
p-キシレン-ビス(N-ピリジニウムブロミド), ≥95% (TLC)
Sigma-Aldrich
塩化ナトリウム 溶液, 0.1 M
Sigma-Aldrich
塩化ナトリウム-35Cl, 99 atom % 35Cl
Sigma-Aldrich
N,N-ジメチルドデシルアミン N-オキシド 溶液, BioUltra, ~0.1 M in H2O