SCF/C-KIT signaling modulates tryptase expression in acute myeloid leukemia cells.

International journal of hematology (2014-05-09)
Jingchun Jin, Yan Li, Yue Wang, Pingping Wang, Yazhu Wang
要旨

Tryptase is a serine protease with a variety of biological functions. Recently, elevated serum tryptase has been detected in certain patients with acute myeloid leukemia (AML). However, the underlying mechanism for the regulation of tryptase expression remains elusive. In this study, we aimed to investigate the role of stem cell factor (SCF)/C-KIT signaling in regulating the expression of tryptase in AML cells. We found a significant positive correlation between tryptase and C-KIT expression levels in AML patients. Furthermore, real-time PCR, Western blot and ELISA analysis showed that SCF upregulated tryptase mRNA and protein expression in U937 cells, and that this effect was abolished by pretreatment with PD98059 and SB230580. In addition, levels of phosphorylated ERK1/2 and p38MAPK correlated with tryptase levels. Taken together, these data suggest that the expression of tryptase is regulated by SCF/C-KIT signaling via the ERK1/2 and p38MAPK pathways.

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フルオロセインイソチオシアナート, アイソマー I, suitable for protein labeling, ≥90% (HPLC), powder
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フルオロセイン 5(6)-イソチオシアナート, BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
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フルオロセイン 5(6)-イソチオシアナート, ≥90% (HPLC)
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フルオロセインイソチオシアナート, アイソマー I, ≥97.5% (HPLC)
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フルオロセインイソチオシアナート, アイソマー I, ≥97.5% (HPLC)
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Anti-p38 MAPK antibody produced in rabbit, affinity isolated antibody
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Monoclonal Anti-MAP Kinase 2 (ERK-2) antibody produced in mouse, clone 1B3B9, purified immunoglobulin, buffered aqueous solution
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Anti-MAPK14 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
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Anti-p38 MAPK antibody produced in rabbit, affinity isolated antibody
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Anti-p38 MAPK antibody produced in rabbit, affinity isolated antibody
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Anti-MAPK14 (Ab-182) antibody produced in rabbit, affinity isolated antibody
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Anti-MAPK14 antibody produced in rabbit, affinity isolated antibody
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Anti-MAP Kinase 2 (ERK-2) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution, ~1 mg/mL
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Anti-p38 MAP Kinase antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

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