Merck
  • A simple and rapid cultural method for detection of Enterobacter sakazakii in environmental samples.

A simple and rapid cultural method for detection of Enterobacter sakazakii in environmental samples.

Journal of food protection (2005-02-05)
O Guillaume-Gentil, V Sonnard, M C Kandhai, J D Marugg, H Joosten
要旨

A method was developed to detect and identify Enterobacter sakazakii in environmental samples. The method is based on selective enrichment at 45+/-0.5 degrees C in lauryl sulfate tryptose broth supplemented with 0.5 M NaCl and 10 mg/liter vancomycin (mLST) for 22 to 24 h followed by streaking on tryptone soy agar with bile salts. When exposed to light during incubation at 37 degrees C, E. sakazakii produces yellow colonies within 24 h; identification was confirmed by testing for alpha-glucosidase activity and by using API 20E strips. All of the E. sakazakii strains tested (n = 99) were able to grow in mLST at 45+/-0.5 degrees C, whereas 35 of 39 strains of potential competitors, all belonging to the Enterobacteriaceae, were suppressed. A survey was carried out with 192 environmental samples from four different milk powder factories. Using this new protocol, E. sakazakii was isolated from almost 40% of the samples, whereas the reference procedure (enrichment in buffered peptone water, isolation on violet red bile glucose agar, and biochemical identification of randomly chosen colonies) only yielded 26% positive results. This selective method can be very useful for the rapid and reliable detection of E. sakazakii in environmental samples.

材料
製品番号
ブランド
製品内容

Millipore
Modified Lauryl Sulfate Tryptose Broth acc.to ISO/TS 22964, suitable for microbiology, NutriSelect® Plus