Microparticle Assembly Pathways on Lipid Membranes.

Biophysical journal (2017-09-07)
Casper van der Wel, Doris Heinrich, Daniela J Kraft
要旨

Understanding interactions between microparticles and lipid membranes is of increasing importance, especially for unraveling the influence of microplastics on our health and environment. Here, we study how a short-ranged adhesive force between microparticles and model lipid membranes causes membrane-mediated particle assembly. Using confocal microscopy, we observe the initial particle attachment to the membrane, then particle wrapping, and in rare cases spontaneous membrane tubulation. In the attached state, we measure that the particle mobility decreases by 26%. If multiple particles adhere to the same vesicle, their initial single-particle state determines their interactions and subsequent assembly pathways: 1) attached particles only aggregate when small adhesive vesicles are present in solution, 2) wrapped particles reversibly attract one another by membrane deformation, and 3) a combination of wrapped and attached particles form membrane-mediated dimers, which further assemble into a variety of complex structures. The experimental observation of distinct assembly pathways, induced only by a short-ranged membrane-particle adhesion, shows that a cytoskeleton or other active components are not required for microparticle aggregation. We suggest that this membrane-mediated microparticle aggregation is a reason behind reported long retention times of polymer microparticles in organisms.

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N-ヒドロキシスルホスクシンイミド ナトリウム塩, ≥98% (HPLC)
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1,2-ジオレオイルsn-グリセロ-3-ホスホコリン, lyophilized powder
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