PURedit™ sgRNA and Cas9 protein offer the best of both worlds. Our SpCas9 modifications offer industry-leading specificity without compromising on-target cutting. Our pre-clinical CRISPR reagents are engineered to meet superior manufacturing standards that enable consistent, high-quality performance every time.
We are so confident in the performance of our PURedit™ products, that we fully guarantee the quality and performance of any gRNA we produce, including custom sequences. If your gRNA does not yield detectable cleavage at the intended target site, we will provide you a one-time replacement, free of charge.
Figure 1. PURedit™ CRISPR RNPs display higher on-target gene editing efficiency and lower off-target cutting in multiple cell models compared to competitors.PURedit™ Cas9, competitor 1 high fidelity variant Cas9, and competitor 2 high efficiency Cas9 were each complexed with PURedit™ sgRNAs and electroporated into both HEK293 and K562 cells. Two PURedit™ sgRNAs targeting two different genes were used with each Cas9 variant. Each transfection contained 5µg of Cas9 protein and 100 pmol of PURedit™ sgRNA. (A) The average percentage of insertions and deletions for the two sgRNAs at their target sites (Indel Activity %) was determined by next generation sequencing (NGS). At both target sites in both cell lines, the PURedit™ RNP complexes showed greater cutting efficiency than competitors. (B) Transfected cells from (A) were analyzed for specificity by quantifying the insertions and deletions at the most active off-target sites, and the ratio of on-target to off-target activity was calculated. PURedit™ Cas9 shows the highest ratio at all targets, confirming high specificity and activity for the intended target sites.
We are so confident in the performance of our PURedit™ products, that we fully guarantee the quality and performance of any gRNA we produce, including custom sequences. If your crRNA or sgRNA do not yield detectable cleavage at the intended target site, we will provide you a one-time replacement, free of charge.
To qualify for this guarantee, please send an image or sequencing data from a single experiment demonstrating detectable cleavage using one of our positive controls, side-by-side with the negative results from your PURedit™ gRNA. To receive your replacement, simply email us and include sample data from a representative experiment (T7E1, TIDE, or NGS).