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[Home](https://www.sigmaaldrich.com/JP/en)[Next-Generation Sequencing](https://www.sigmaaldrich.com/JP/en/applications/genomics/next-gen-sequencing)SeqPlex Enhanced DNA Amplification Kit (SEQXE) Protocol
# SeqPlex Enhanced DNA Amplification Kit (SEQXE) Protocol
[Product Description](https://www.sigmaaldrich.com#description)
[Workflow](https://www.sigmaaldrich.com#workflow)
[Reagents Provided](https://www.sigmaaldrich.com#reagents)
[Storage/Stability](https://www.sigmaaldrich.com#storage)
[Procedure](https://www.sigmaaldrich.com#procedure)
[Appendix 1: Optional Primer Removal Assay](https://www.sigmaaldrich.com#appendix)
[Troubleshooting Guide](https://www.sigmaaldrich.com#trouble)
[FAQs](https://www.sigmaaldrich.com#faq)
[Materials](https://www.sigmaaldrich.com#materials)
## [](https://www.sigmaaldrich.com)Product Description
The SeqPlex DNA Amplification Kit ([SEQXE](https://www.sigmaaldrich.com/JP/en/product/sigma/SEQXE)) for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA. The yields from chromatin immunoprecipitation (ChIP) or formalin-fixed paraffin-embedded tissue samples (FFPE) are often less than required for successful NGS library preparation. The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented DNA samples for input into a NGS workflow.
The SeqPlex process is comprised of three steps: pre-amplification, amplification and primer removal. See SeqPlex Process Workflow chart below.
In the first step, Pre-Amplification, the DNA is replicated using primers composed of a semi-degenerate 3’-end and a universal 5’-end. As polymerization proceeds, displaced single strands serve as new templates for additional primer annealing and extension. The Pre-Amplification product is composed of random, overlapping amplicons which are flanked by a universal end sequence.
In the second step, Amplification, the product of Pre-Amplification is amplified by single primer PCR via the universal end sequence. The product of SeqPlex amplification ranges from 200 to 500 base pairs. Amplicons from ChIP and/or degraded DNA, such as FFPE, are typically shorter and dependent upon length of the starting DNA.
The third step, Primer Removal, removes the primer and semi-degenerate sequences from the amplicon pool making SeqPlex DNA ready to enter a NGS workflow.
## [](https://www.sigmaaldrich.com)Reagents Provided
| | | | | |
|----------------------------------|-----------------------------------------------------------------|--------|---------|---------|
| Reagent | Catalog No. | 10 RXN | 50 RXN | 500 RXN |
| Library Preparation Buffer | LP100 | 20 µL | 100 µL | 1000 µL |
| Library Preparation Enzyme | E0531 | 10 µL | 50 µL | 500 µL |
| 5x Amplification Mix | A5112 | 235 µL | 1.15 mL | 11.5 mL |
| DNA Polymerase for SeqPlex | SP300 | 20 µL | 100 µL | 1 mL |
| 10x Primer Removal Buffer | SR401 | 165 µL | 825 µL | 8.2 mL |
| Primer Removal Enzyme | SR402 | 42 µL | 210 µL | 2 mL |
| Primer Removal Solution | SR400 | 33 µL | 165 µL | 1.6 mL |
| Water, Molecular Biology Reagent | [W4502](https://www.sigmaaldrich.com/JP/en/product/sigma/W4502) | 5 mL | 25 mL | 220mL |
## [](https://www.sigmaaldrich.com)Storage/Stability
All components should be stored at –20 °C. When thawed for use, components should be kept on ice. Dissolve any precipitate in these solutions by briefly heating at 37 °C, with thorough mixing. Stability of the Library Preparation Enzyme and Primer Removal Enzyme will be affected if stored above –20 °C or allowed to remain for long periods at temperatures over 4 °C
## [](https://www.sigmaaldrich.com)Procedure
The following procedure has been used successfully to amplify and sequence 100 pg of ChIP-isolated DNA and 100 pg of fragmented genomic DNA. Damaged DNA, such as that isolated from FFPE tissues, may require 5- to 10-fold more input DNA, depending on the degree of damage. Reactions can be scaled up or down to accommodate preparation of needed quantities of amplified DNA.
__Note:__ Final yield after amplification and primer removal varies significantly depending upon the quality of starting DNA. In most cases, 1 to 2 μg can be expected. Perform multiple reactions for larger quantities.
__Note:__ This procedure was developed using the specific reagents provided with, or recommended for use with, this kit. Substitutions may result in suboptimal results.
#### Pre-amplification
1. For the best SeqPlex amplification coverage and NGS results, the optimum starting DNA size is 200-500bp. DNA from many ChIP and FFPE samples are already within this size range. Such samples do not require additional fragmentation prior to step 4 of the SeqPlex protocol.
__If starting DNA is not within the size range of 200-500 bp, fragmentation is strongly recommended.__ DNA may be fragmented using sonication, a Covaris instrument, or enzymatic fragmentation.
2. If possible, determine the concentration of DNA by UV absorption (260 nm), and use up to 1 ng per reaction.
3. Thaw the Library Preparation Buffer (LP100), 5x Amplification Buffer (A5112) and water ([W4502](https://www.sigmaaldrich.com/JP/en/product/sigma/W4502)). Mix thoroughly.
4. Combine as follows:
2 µL of __Library Preparation Buffer (LP100)__
X µL DNA (up to 1 ng)
Y µL water ([W4502](https://www.sigmaaldrich.com/JP/en/product/sigma/W4502))
--------------------------------------------------
14 µL Total reaction volume
__Caution—Experienced GenomePlex® WGA users:__ Several components found in the SeqPlex Enhanced DNA Amplification kit (SEQXE), SeqPlex DNA Amplification kit (SEQX), GenomePlex WGA kits, Transplex® WTA1 kit and Complete Whole Transcriptome Amplification Kit (WTA2) are similarly named. Though generally analogous in function, __they are not interchangeable.__
5. Mix thoroughly, centrifuge briefly, and incubate in a thermal cycler programmed for:
95 °C for 2 minutes
4 °C Hold
6. After the sample has cooled to 4 °C, add 1 µL of __Library Preparation Enzyme (E0531)__. Cap tube and mix thoroughly. Centrifuge briefly and immediately proceed to next step.
7. Place reaction in a thermal cycler and incubate as
follows:
16 °C for 20 minutes
24 °C for 20 minutes
37 °C for 20 minutes
75 °C for 5 minutes
4 °C Hold
8. Remove sample from thermal cycler and centrifuge briefly. Amplification may be completed immediately or store Pre-Amplification product at –20 °C for up to three days.
__Caution—Experienced GenomePlex WGA users:__
__· SeqPlex uses a 5x Amplification Mix__
__· SYBR Green ([S9430](https://www.sigmaaldrich.com/JP/en/product/sial/S9430)) is recommended to monitor the amplification__
__· Annealing/Extension temperature is 70 oC__
__· A 30-minute 70 oC post-amplification hold is required__
9. Add the following reagents to the 15 mL of Pre-Amplification product from step 8. (For multiple reactions, a master mix may be prepared. Add 60 mL of the master mix to each reaction):
15 µL 5X __Amplification Mix__ (A5112)
1.5 µL __DNA Polymerase for SeqPlex__ (SP300)
42.5 µL __Water__ ([W4502](https://www.sigmaaldrich.com/JP/en/product/sigma/W4502))
1 µL SYBR Green I ([S9430](https://www.sigmaaldrich.com/JP/en/product/sial/S9430)) diluted 1/1000*
- µL Instrument Specific Reference Dye (optional)
--------------------------------------------------
75 µL Total reaction volume
\*For the best representation, real-time PCR with addition of SYBRGreen I to the amplification reaction is strongly recommended to enable monitoring of the reaction progress. __SYBRGreen I ([S9430](https://www.sigmaaldrich.com/JP/en/product/sial/S9430)) must be diluted 1,000-fold (1/1000) and 1µl used per 75 µl SeqPlex amplification reaction to avoid inhibiting the amplification reaction.__ SYBRGreen formulations other than S9430 have not been tested and are not recommended.
Optimal results are achieved by proceeding at least 2–3 cycles after the start of the amplification “plateau”, as indicated on Figure 1. The optimal number of amplification cycles varies with starting DNA template quantity and quality.
If amplification is performed without adding SYBRGreen I, 18 to 24 cycles usually gives good results with 0.1-1.0 ng of high quality DNA. Low quality DNA may require higher input quantities and/or more cycles. If input amounts are near
10 pg, as many as 29 cycles may be required to reach amplification plateau.
__Note:__ If more than 29 cycles are required to achieve plateau, subsequent NGS results may be unsatisfactory. Consult the Troubleshooting Guide.
10\. Mix thoroughly and cycle in a real-time thermal cycler as follows:
Initial Denaturation: 94 °C for 2 minutes
Cycle until 2-3 cycles into plateau:
94 °C Denature for 15 seconds
70 °C Anneal/Extend for 5 minutes
(read fluorescence)
After cycling:
70 °C for 30 minutes
4 °C Hold
__Note: The extended incubation at 70°C after cycling is absolutely essential for efficient primer removal.__
Reactions may be purified immediately or stored at –20 °C until purification.
11\. Purify using GenElute PCR Clean-Up Kit (NA1020). Elute in 50 µL nuclease-free water.
12\. Determine the purified DNA’s concentration by measuring the absorbance at 260nm. One A260 unit is equivalent to 50ng/µL DNA. Yield at this point will vary depending on the quality of starting DNA, but is usually 1-5 µg.
__Note:__ Alternative measurement techniques, such as PicoGreen®, will often underestimate the actual SeqPlex DNA yield.
At this point, the SeqPlex DNA is suitable for qPCR and microarrays. SeqPlex primer removal is recommended for deep sequencing.
Optional: Amplification quality may be assessed by gel or capillary electrophoresis. Typically, 1mL of crude or purified amplification product is sufficient for capillary chips, such as those for Agilent’s Bioanalyzer, while 5 mL is usually sufficient for agarose gel electrophoresis.
#### Primer Removal
__Caution—Experienced GenomePlex WGA users:__
SeqPlex Enhanced (SEQXE) Primer Removal reagents will not work on DNA amplified with previous generations of SeqPlex (SEQX) or GenomePlex (WGA1-5).
A reaction input of 2.1 μg is recommended to yield sufficient product for entering most deep sequencing workflows (>1μg).
13\. Combine the following on ice:
8.0 µL - 10x Primer Removal Buffer (SR401)
1.6 µL - Primer Removal Solution (SR400)
X µL – 2.1 μg of purified SeqPlex DNA
Y µL - of Water ([W4502](https://www.sigmaaldrich.com/JP/en/product/sigma/W4502))
--------------------------------------------------
76.25 µL Total reaction volume
14\. Mix, and if performing the Optional Primer Removal Assay (see Appendix 1 below), remove 5 mL into a separate tube for “without primer removal enzyme” control.
15\. Add 3.75 µL - Primer Removal Enzyme (SR402) to the remaining 71.25 µL from step 13.
16\. Incubate both ± enzyme reactions as follows:
37 °C for 60 minutes
65 °C for 20 minutes
4 °C Hold
17\. Remove reactions from the thermocycler, centrifuge briefly, and mix. Reactions may be stored at –20 °C for up to three days or purified immediately.
18\. If performing the Optional Primer Removal Assay, remove and save 5 µL from the “with primer removal enzyme” reaction (see Appendix 1 below) prior to purification.
19\. Purify the remaining reaction with primer removal enzyme using the GenElute PCR Clean-up Kit as described previously in step 10.
## Sequencing
The SeqPlex DNA is now ready to enter Next-Generation Sequencing work flows, including end preparation, but generally does not require fragmentation or size selection. The bulk of SeqPlex DNA ranges from 200 to 500 base pairs. Though typically unnecessary, additional fragmentation can be accomplished mechanically or enzymatically.
Each SeqPlex amplicon terminus will have a 5’-phosphate and a 2-base 3’-over-hang after primer removal. Prior to ligation of sequencing primers, polish double-stranded fragment ends with T4 polymerase.
Any SeqPlex amplicons retaining primer after primer removal treatment will have a 5’-hydroxyl. T4 DNA kinase treatment is not required, and its omission will reduce ligation of sequencing primers to any residual undigested SeqPlex product.
The 3’-adenylation in Illumina’s workflow largely prevents ligation products, such as chimeras, from occurring. Chimeras have been reported with Roche 454 library preparation and sequencing. For ABI SoLID sequencing, a second sizing step prior to sequencing may help to reduce chimeras or concatemers.
## Product Profile
This kit has been demonstrated to amplify ChIP DNA samples and whole genomic fragmented DNA templates. Note that a specific sequence within a highly complex DNA sample, such as ChIP input DNA, may not amplify to the same extent as that same sequence within a less complex DNA sample, such as ChIPed DNA. Therefore, representation levels are best compared between samples of similar complexity, such as ChIPed DNA from treated and untreated cells or tumor and normal tissue.
SeqPlex DNA has been successfully prepared for sequencing on Illumina’s GAIIx and MiSeq using standard Illumina workflow protocols including: end preparation, polishing, amplification and size selection.
We strongly recommend confirming the quality of SeqPlex DNA after primer removal by performing PCR with primers to short (<300bp) DNA sequences known to be present in the starting DNA before submitting samples for next generation sequencing.
__Note:__ PCR products or primers containing the sequence 5’-CTGAAG-3’ or 5’-CTTCAG-3’ are not expected to amplify after primer removal.
Example of PCR product that WILL NOT amplify after primer removal:
The presence of specific targets within SeqPlex DNA may also be confirmed by PCR or microarray before or after primer removal.
The stability of the SeqPlex DNA is equivalent to genomic DNA stored under identical conditions.
## [](https://www.sigmaaldrich.com)Appendix 1
Optional: Primer Removal Assay
The efficiency of primer removal can be assessed by performing qPCR using the 5X Amplification Mix (A5112), DNA Polymerase for SeqPlex (SP300), and the 5 µL retained samples from steps 18 &14 above for “with” and “without primer removal enzyme”. Sufficient 5X Amplification Mix and DNA Polymerase is provided to perform one Primer Removal Assay, “with” and “without primer removal enzyme”, for each amplification reaction.
1. Serially dilute both “with” and “without primer removal” enzyme samples 1,000,000-fold (eg, 1 µL into 99 µL water, 3 times consecutively).
2. Prepare assay mix for both by combining:
6.6 µL 5X Amplification Mix (A5112)
0.33 µL DNA Polymerase for SeqPlex (SP300)
0.33 µL 1/1,000 dilution, SYBR Green (S9430) in water
3.75 µL Water ([W4502](https://www.sigmaaldrich.com/JP/en/product/sigma/W4502))
__Note:__ Reagents requiring small volumes per assay may be diluted with water prior to addition to avoid measurement inaccuracies or an assay master mix may be prepared for multiple tests.
3. Pipet 10 µL of both 1,000,000 fold diluted SeqPlex “with” and “without” enzyme into a separate PCR tubes or wells.
4. Combine 5 µL assay mix from step 2 with each 10 µL of the 1,000,000 diluted SeqPlex products from step 3.
5. Amplify as follows:
94° C for 2 minutes.
30 - 35 cycles*
94° C for 15 seconds
70° C for 5 minutes (read)
A Ct/Cq for the “with” Primer Removal Enzyme reaction that is at least 4 cycles greater than that for the “without” enzyme reaction indicates successful primer removal.
### Reference
1\.
Lo R, Matthews J. 2012. High-Resolution Genome-wide Mapping of AHR and ARNT Binding Sites by ChIP-Seq. 130(2):349-361. [https://doi.org/10.1093/toxsci/kfs253](https://doi.org/10.1093/toxsci/kfs253)
### Troubleshooting Guide
ObservationCauseRecommended Solution
No product was detected after amplification.Incorrect Annealing/Extension temperature or timePerform reaction again with 70 °C Annealing/Extension for 5 minutes each cycle.
Too few PCR cycles during AmplificationPerform reaction again with more cycles (up to 29) and monitor amplification with SYBR Green in a real-time thermal cycler
Starting DNA was insufficient or too severely degradedPerform reaction again with more starting DNA
No amplification curve was seen during real-time PCR (qPCR) monitoringSYBR Green was not addedSYBR Green I, Catalog No. S9430, is not included with SeqPlex reagents, but must be added to monitor real-time PCR.
A reference dye specific to the qPCR instrument may be requiredAdd instrument specific reference dye. If adding reference dye is not possible, excess cycles should be completed to ensure complete cycling. See FAQ for additional assistance.
Excessive (>60%) or complete SeqPlex DNA loss detected after primer removalPoor recovery during clean-up Repeat digest and perform clean-up with GenElute PCR Clean-up Kit, making sure to use a full 5 volumes of binding solution, to dilute wash solution with the correct volume of ethanol, and to properly dry the column before elution.
Primer Removal Enzyme is contaminated with nucleaseUse a new aliquot of Primer Removal Enzyme (SR402) or if new aliquot not available, purchase new SeqPlex Enhanced DNA Amplification Kit (SEQXE)
SeqPlex product concentration too low for detection methodUse specific qPCR primers with a standard curve made from the SeqPlex DNA before primer removal to estimate SeqPlex concentration after primer removal. __Note:__ Primers that include 5’-CTGAAG-3’ or 5’-CTTCAG-3’ in the template are not compatible with PCR after primer removal.
PCR check before Primer Removal worked, but PCR check after failedPrimers for PCR check amplify a sequence that was cleaved during primer removalSelect new PCR primers that do not flank a region containing 5’-CTGAAG-3’ or 5’-CTTCAG-3’ for amplification.
SeqPlex DNA was completely degraded during Primer RemovalSee above for “Excessive (>60%) or complete SeqPlex DNA loss detected after primer removal”
Poor representation after NGSSeqPlex input DNA not sheared to 200-500bpVerify size and fragment if necessary prior to starting the SeqPlex procedure.
Not enough cycles during SeqPlex AmplificationMonitor amplification cycling and cycle at least 2-3 cycles past plateau. If monitoring completely fails, a default 29 cycles may be used.
NGS IVC abnormalitiesIVC plots show identical sequence (primer) for the first few nucleotides in multiple readsOptimum instrument cluster calling may be achieved by normalizing the run to a lane that does not contain SeqPlex DNA.
## [](https://www.sigmaaldrich.com)Frequently Asked Questions
01. __Is SeqPlex DNA compatible with microarrays and qPCR?__ Yes, SeqPlex DNA, with or without primer removal, may be used in these applications like genomic DNA or existing GenomePlex products.
02. __Are there advantages to SeqPlex over GenomePlex if primer removal is not needed?__ SeqPlex Pre-Amplification primers have been designed to target more frequently than existing GenomePlex WGA primers and therefore may provide the advantage of superior genome coverage in some regions.
03. __Will reducing cycles during amplification improve representation?__ No, Cycling at least 2-3 cycles beyond the "plateau" achieves optimum representation. Insufficient cycling leads to a significant reduction in representation/coverage.
04. __If monitoring amplification cycling via real-time PCR is not possible, how many cycles should be run?__ When optimum cycling conditions cannot be determined by monitoring, over-cycling is suggested. For 100 pg-1 ng ChIP samples; 24 cycles should be sufficient. For inputs less than 100 pg or poor quality; 29 cycles should be sufficient.
05. __Will the SeqPlex primer remaining after primer removal interfere with Next-Generation sequencer cluster calling?__ No, SeqPlex DNA with a minimal amount of primer remaining will generate excellent sequence on an Illumina GAIIx and MiSeq. To achieve optimum cluster calling of SeqPlex DNA, see Troubleshooting Guide for “NGS IVC abnormalities.”
06. __Will the SeqPlex Primer Removal Enzyme remove the entire SeqPlex primer?__ Yes, the SeqPlex Primer Removal Enzyme removes the complete primer, but the digestion may not be 100% complete.
07. __Will all copies of the SeqPlex primer be completely removed?__ A small amount of complete SeqPlex primer may remain. Most of the SeqPlex DNA (>90%) will have the SeqPlex primer completely removed.
08. __What yield should be expected?__ Yields are highly variable and dependent on a number of factors. Staring with 100 pg of ChIP DNA, roughly 1-5 ug of Amplification product and 1-3 µg of SeqPlex DNA ready for NGS library preparation is expected from each primer removal reaction.
__Note:__ Up to 50% product reduction should be anticipated with primer removal. Therefore, if >1 μg is needed for sequencing, multiple separate 2 μg Primer Removal reactions should be performed.
09. __May the GenomePlex Reamplification kit (WGA3) be used with SeqPlex?__ No, the SeqPlex DNA before and after primer removal is incompatible with the GenomePlex reamplification kit. Primer sequences differ.
10. __Will SeqPlex DNA require special NGS sequencing protocols?__ No, SeqPlex DNA fits directly into NGS workflows, just like ChIP DNA. Sequencing instrument operators should be notified of running SeqPlex DNA and to expect a slight signal from any remaining primers in the IVC plots.
## Materials
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- [エンドヌクレアーゼ](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/bioprocessing-formulation-raw-materials/endonuclease)
- [mRNA製造向け高品質化学品](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/bioprocessing-formulation-raw-materials/high-quality-chemicals-for-mrna-manufacturing)
- [アップストリームプロセス用医薬品原材料](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/bioprocessing-formulation-raw-materials/upstream-process-chemicals)
- [バイオプロセッシング細胞培養](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/bioprocessing-cell-culture)
- [バイオプロセス細胞培養培地](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/bioprocessing-cell-culture/bioprocessing-cell-culture-media)
- [バイオプロセッシング細胞株](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/bioprocessing-cell-culture/bioprocessing-cell-lines)
- [バイオプロセッシング液体細胞培地・バッファー](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/bioprocessing-formulation-raw-materials/liquid-cell-culture-media-buffers)
- [バイオプロセッシング組換え添加剤](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/bioprocessing-cell-culture/recombinant-supplements)
- [バルク・粉末輸送バッグ](https://www.sigmaaldrich.com/JP/ja/services/custom-products/bulk-powder-transfer-bags)
- [細胞保持](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/bioprocessing-cell-culture/cell-retention)
- [カスタムGMP細胞培養培地](https://www.sigmaaldrich.com/JP/ja/services/custom-products/custom-gmp-cell-culture-products)
- [培地ろ過](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/buffer-media-prep/media-filtration)
- [ウイルスベクターアップストリームプロセス](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/bioprocessing-cell-culture/viral-vector-upstream-platforms)
- [バイオプロセスシステム](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/bioprocessing-systems)
- [バイオプロセッシング用クロマトグラフィーカラム](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/chromatography-bioprocessing/chromatography-columns)
- [クロマトグラフィーシステム](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/chromatography-bioprocessing/chromatography-systems)
- [清澄化フィルターホルダー・システム](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/clarification-harvest/clarification-filter-holders-and-systems-for-bioprocessing)
- [完全性テスター](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/sterile-filtration-and-bioburden-control/integrity-testers)
- [シングルユースミキサー](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/buffer-media-prep/single-use-mixers)
- [ステンレス製コネクタ・バルブ・ミキサー](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/fluid-management/stainless-steel-connectors-valves-mixers)
- [タンジェンシャルフロー・フィルトレーションホルダー/システム](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/ultrafiltration-diafiltration/tangential-flow-holders-systems)
- [ウイルスフィルターホルダー・システム](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/viral-clearance/virus-filter-holders-and-systems)
- [バッファー・培地調製](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/buffer-media-prep)
- [バッファー・pH調整剤](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/buffer-media-prep/buffers-and-ph-adjusters)
- [バッファーろ過](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/buffer-media-prep/buffer-filtration)
- [培地ろ過](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/buffer-media-prep/media-filtration)
- [シングルユースミキサー](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/buffer-media-prep/single-use-mixers)
- [クロマトグラフィー](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/chromatography-bioprocessing)
- [アフィニティクロマトグラフィーレジン](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/chromatography-bioprocessing/affinity-resins)
- [バイオプロセッシング用クロマトグラフィーカラム](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/chromatography-bioprocessing/chromatography-columns)
- [クロマトグラフィーシステム](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/chromatography-bioprocessing/chromatography-systems)
- [イオン交換クロマトグラフィーレジン](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/chromatography-bioprocessing/ion-exchange-chromatography)
- [メンブレンクロマトグラフィー](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/chromatography-bioprocessing/membrane-chromatography)
- [逆相/順相クロマトグラフィーレジン](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/chromatography-bioprocessing/reverse-normal-phase-chromatography)
- [サイズ排除クロマトグラフィーレジン](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/chromatography-bioprocessing/size-exclusion-chromatography)
- [清澄化・ハーベスト](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/clarification-harvest)
- [清澄化デプスフィルター・前処理](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/clarification-harvest/clarification-depth-filters-pretreatment-solutions)
- [清澄化フィルターホルダー・システム](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/clarification-harvest/clarification-filter-holders-and-systems-for-bioprocessing)
- [清澄化プレフィルター](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/clarification-harvest/clarification-prefilters)
- [流体マネージメント](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/fluid-management)
- [バイオプロセスにおける接続/切断](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/fluid-management/connection-disconnection-for-bioprocessing)
- [流体輸送・保存](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/fluid-management/fluid-transport-and-storage)
- [シングルユースアセンブリ](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/fluid-management/single-use-assemblies)
- [シングルユースミキサー](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/buffer-media-prep/single-use-mixers)
- [ステンレス製コネクタ・バルブ・ミキサー](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/fluid-management/stainless-steel-connectors-valves-mixers)
- [化学式](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/formulation)
- [活性・官能化PEG](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/formulation/activated-functionalized-pegs)
- [生体分子製剤](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/formulation/biomolecule-formulation)
- [顆粒状材料](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/formulation/granulated-materials)
- [注射製剤・非経口製剤](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/formulation/injectables-parenteral-formulations)
- [液体製剤](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/formulation/liquid-formulation)
- [タンパク質安定化剤](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/formulation/protein-stabilizers)
- [半固形製剤](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/formulation/semisolid-formulation)
- [固形製剤](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/formulation/solid-formulation)
- [PEG脂質](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/formulation/synthetic-lipids)
- [粘度低減プラットホーム](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/formulation/viscosity-reduction-platform)
- [滅菌・無菌サンプリングソリューション](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/sterile-and-aseptic-sampling)
- [滅菌プロセスサンプリング](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/sterile-and-aseptic-sampling/manual-sterile-sampling)
- [MAST<sup>®</sup>オートサンプリングソリューションによるサンプリング](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/sterile-and-aseptic-sampling/automated-aseptic-sampling)
- [無菌ろ過・バイオバーデン管理](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/sterile-filtration-and-bioburden-control)
- [バッファーろ過](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/buffer-media-prep/buffer-filtration)
- [フィルターハウジング](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/sterile-filtration-and-bioburden-control/filter-housings)
- [最終滅菌ろ過・充填](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/sterile-filtration-and-bioburden-control/final-sterile-filtration-and-filling)
- [ガス・ベントフィルター](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/sterile-filtration-and-bioburden-control/gas-and-vent-filters)
- [完全性テスター](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/sterile-filtration-and-bioburden-control/integrity-testers)
- [培地ろ過](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/buffer-media-prep/media-filtration)
- [プレフィルター・バイオバーデン管理](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/sterile-filtration-and-bioburden-control/prefilters-and-bioburden-control)
- [滅菌フィルター](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/sterile-filtration-and-bioburden-control/sterile-filters)
- [限外ろ過・ダイアフィルトレーション](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/ultrafiltration-diafiltration)
- [タンジェンシャルフローフィルトレーション装置](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/ultrafiltration-diafiltration/filtration-devices)
- [タンジェンシャルフローフィルトレーションホルダー・システム](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/ultrafiltration-diafiltration/tangential-flow-holders-systems)
- [ウイルスクリアランス](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/viral-clearance)
- [完全性テスター](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/sterile-filtration-and-bioburden-control/integrity-testers)
- [ウイルスフィルターホルダー・システム](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/viral-clearance/virus-filter-holders-and-systems)
- [ウイルスフィルター](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/viral-clearance/virus-filters)
- [ウイルス不活化](https://www.sigmaaldrich.com/JP/ja/products/pharma-and-biopharma-manufacturing/viral-clearance/virus-inactivation)
- [タンパク質生物学](https://www.sigmaaldrich.com/JP/ja/products/protein-biology)
- [抗体](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/antibodies)
- [高度なバリデーション抗体](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/antibodies/enhanced-validation-ab)
- [フローサイトメトリー用抗体](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/antibodies/flow-cytometry-antibodies)
- [Prestige Antibodies<sup>®</sup>](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/antibodies/prestige-antibodies)
- [一次抗体](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/antibodies/primary-antibodies)
- [二次抗体](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/antibodies/secondary-antibodies)
- [スモールパック抗体](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/antibodies/small-pack-antibodies)
- [ZooMAb<sup>®</sup>リコンビナント抗体](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/antibodies/zoomab-antibodies)
- [低分子生理活性物質](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/bioactive-small-molecules)
- [構造ゲノミクスコンソーシアム](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/bioactive-small-molecules/structural-genomics-consortium)
- [Duolink<sup>™</sup> PLA-タンパク質相互作用検出](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/duolink-proximity-ligation-assay)
- [イムノアッセイプラットフォーム](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/immunoassay-platform-solutions)
- [ELISAキット](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/immunoassay-platform-solutions/elisa-kits)
- [MILLIPLEX<sup>®</sup>キット](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/immunoassay-platform-solutions/milliplex-multiplex-assays-for-luminex-technology)
- [SMC<sup>®</sup>技術](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/immunoassay-platform-solutions/single-molecule-counting-technology)
- [タンパク質電気泳動・ウェスタンブロッティング](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-electrophoresis-and-western-blotting)
- [電気泳動・転写装置](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-electrophoresis-and-western-blotting/electrophoresis-and-transfer-equipment)
- [トランスファーメンブレン・キット](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-electrophoresis-and-western-blotting/transfer-membranes-and-kits)
- [免疫検出試薬・基質](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-electrophoresis-and-western-blotting/immunodetection-reagents-and-substrates)
- [メンブレン免疫検出デバイス](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-electrophoresis-and-western-blotting/membrane-immunodetection-devices)
- [タンパク質電気泳動用ゲル・バッファー](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-electrophoresis-and-western-blotting/protein-electrophoresis-gels-and-buffers)
- [タンパク質電気泳動法用ゲル染色剤](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-electrophoresis-and-western-blotting/protein-electrophoresis-gel-stains)
- [プロテインマーカー](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-electrophoresis-and-western-blotting/protein-markers)
- [転写用バッファー・試薬](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-electrophoresis-and-western-blotting/transfer-buffers-and-reagents)
- [タンパク質・酵素](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/proteins-and-enzymes)
- [アルブミン・輸送タンパク質](https://www.sigmaaldrich.com/JP/ja/products/cell-culture-and-analysis/cell-culture-supplements-and-reagents/albumins-and-transport-proteins)
- [補酵素類](https://www.sigmaaldrich.com/JP/ja/products/chemistry-and-biochemicals/biochemicals/coenzymes)
- [検出用基質・酵素](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/proteins-and-enzymes/detection-substrates-and-enzymes)
- [薬物分析酵素](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/proteins-and-enzymes/drug-analysis-enzymes)
- [グリコバイオロジープロファイリングツール](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/proteins-and-enzymes/glycosidases)
- [ヌクレアーゼ](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/proteins-and-enzymes/nucleases)
- [プロテアーゼ](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/proteins-and-enzymes/proteases)
- [組織分離](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/proteins-and-enzymes/tissue-dissociation)
- [タンパク質質量分析](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-mass-spectrometry)
- [タンパク質精製クロマトグラフィー](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-purification-chromatography)
- [抗体精製](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-sample-prep/antibody-protein-purification)
- [ビオチン化タンパク質精製](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-sample-prep/biotinylated-protein-purification)
- [FLAG<sup>®</sup>精製](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-sample-prep/flag-purification)
- [遺伝子組換え/融合タグタンパク質精製](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-sample-prep/recombinant-fusion-tag-protein-purification)
- [タンパク質サンプル調製](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-sample-prep)
- [Amicon<sup>™</sup>遠心式フィルター](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-sample-prep/amicon-centrifugals)
- [抗体精製](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-sample-prep/antibody-protein-purification)
- [ビオチン化タンパク質精製](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-sample-prep/biotinylated-protein-purification)
- [FLAG<sup>®</sup>精製](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-sample-prep/flag-purification)
- [インヒビターカクテル](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-sample-prep/inhibitor-cocktails)
- [ラボ用濃縮用デバイス](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-sample-prep/laboratory-concentration-devices)
- [ラボ用透析・ろ過デバイス](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-sample-prep/laboratory-dialysis-and-diafiltration-devices)
- [遺伝子組換え/融合タグタンパク質精製](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-sample-prep/recombinant-fusion-tag-protein-purification)
- [細胞分画試薬](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-sample-prep/subcellular-fractionation-reagents)
- [細胞溶解試薬・エンハンサー](https://www.sigmaaldrich.com/JP/ja/products/protein-biology/protein-sample-prep/whole-cell-lysis-reagents-and-enhancers)
- [超純水・純水製造装置](https://www.sigmaaldrich.com/JP/ja/products/water-purification)
- [Milli-Q<sup>®</sup>超純水・純水製造装置](https://www.sigmaaldrich.com/JP/ja/products/water-purification/milli-q-benchtop-water-purification-systems)
- [Milli-Q<sup>®</sup>高流量純水製造装置](https://www.sigmaaldrich.com/JP/ja/products/water-purification/milli-q-high-flow-water-purification-systems)
- [Milli-Q<sup>®</sup>消耗品](https://www.sigmaaldrich.com/JP/ja/products/water-purification/milli-q-consumables)
- [Milli-Q<sup>®</sup>アクセサリー](https://www.sigmaaldrich.com/JP/ja/products/water-purification/milli-q-accessories)
- アプリケーション
- [分析化学](https://www.sigmaaldrich.com/JP/ja/applications/analytical-chemistry)
- [原子分光分析](https://www.sigmaaldrich.com/JP/ja/applications/analytical-chemistry/atomic-spectroscopy)
- [キャリブレーション・クオリフィケーション・バリデーション](https://www.sigmaaldrich.com/JP/ja/applications/analytical-chemistry/calibration-qualification-and-validation)
- [ガスクロマトグラフィー (GC)](https://www.sigmaaldrich.com/JP/ja/applications/analytical-chemistry/gas-chromatography)
- [液体クロマトグラフィー (HPLC)](https://www.sigmaaldrich.com/JP/ja/applications/analytical-chemistry/liquid-chromatography)
- [高分子用HPLC](https://www.sigmaaldrich.com/JP/ja/applications/analytical-chemistry/liquid-chromatography/large-molecule-hplc)
- [吸着・イオン交換樹脂・クロマト用担体](https://www.sigmaaldrich.com/JP/ja/applications/analytical-chemistry/liquid-chromatography/low-pressure-liquid-chromatography)
- [低分子用HPLC](https://www.sigmaaldrich.com/JP/ja/applications/analytical-chemistry/liquid-chromatography/small-molecule-hplc)
- [質量分析 (MS)](https://www.sigmaaldrich.com/JP/ja/applications/analytical-chemistry/mass-spectrometry)
- [核磁気共鳴 (NMR)](https://www.sigmaaldrich.com/JP/ja/applications/analytical-chemistry/nuclear-magnetic-resonance)
- [測光分析・反射光分析](https://www.sigmaaldrich.com/JP/ja/applications/analytical-chemistry/photometry-and-reflectometry)
- [分析前処理](https://www.sigmaaldrich.com/JP/ja/applications/analytical-chemistry/sample-preparation)
- [サンプルろ過](https://www.sigmaaldrich.com/JP/ja/applications/analytical-chemistry/sample-preparation/filtration)
- [サンプル精製](https://www.sigmaaldrich.com/JP/ja/applications/analytical-chemistry/sample-preparation/purification)
- [QuEChERSサンプル前処理](https://www.sigmaaldrich.com/JP/ja/applications/analytical-chemistry/sample-preparation/quechers)
- [固相抽出](https://www.sigmaaldrich.com/JP/ja/applications/analytical-chemistry/sample-preparation/solid-phase-extraction)
- [固相マイクロ抽出 (SPME)](https://www.sigmaaldrich.com/JP/ja/applications/analytical-chemistry/sample-preparation/solid-phase-microextraction)
- [薄層クロマトグラフィー (TLC)](https://www.sigmaaldrich.com/JP/ja/applications/analytical-chemistry/thin-layer-chromatography)
- [滴定・カールフィッシャー滴定法](https://www.sigmaaldrich.com/JP/ja/applications/analytical-chemistry/titration-and-karl-fischer)
- [化学分析に適した水](https://www.sigmaaldrich.com/JP/ja/applications/water-purification/water-analytical-chemistry)
- [ウェットケミカル分析](https://www.sigmaaldrich.com/JP/ja/applications/analytical-chemistry/wet-chemical-analysis)
- [細胞培養・細胞解析](https://www.sigmaaldrich.com/JP/ja/applications/cell-culture-and-cell-culture-analysis)
- [細胞解析](https://www.sigmaaldrich.com/JP/ja/applications/cell-culture-and-cell-culture-analysis/cell-analysis)
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- [イメージング解析・ライブセルイメージング](https://www.sigmaaldrich.com/JP/ja/applications/cell-culture-and-cell-culture-analysis/cell-analysis/imaging-analysis-and-live-cell-imaging)
- [細胞タイプ別細胞培養](https://www.sigmaaldrich.com/JP/ja/applications/cell-culture-and-cell-culture-analysis/cell-culture-by-cell-type)
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- [微生物細胞培養](https://www.sigmaaldrich.com/JP/ja/applications/cell-culture-and-cell-culture-analysis/cell-culture-by-cell-type/microbial-cell-culture)
- [植物組織培養](https://www.sigmaaldrich.com/JP/ja/applications/cell-culture-and-cell-culture-analysis/cell-culture-by-cell-type/plant-tissue-culture)
- [初代細胞培養](https://www.sigmaaldrich.com/JP/ja/applications/cell-culture-and-cell-culture-analysis/cell-culture-by-cell-type/primary-cell-culture)
- [幹細胞培養](https://www.sigmaaldrich.com/JP/ja/applications/cell-culture-and-cell-culture-analysis/cell-culture-by-cell-type/stem-cell-culture)
- [手法別細胞培養](https://www.sigmaaldrich.com/JP/ja/applications/cell-culture-and-cell-culture-analysis/cell-culture-by-technique)
- [3D細胞培養](https://www.sigmaaldrich.com/JP/ja/applications/cell-culture-and-cell-culture-analysis/cell-culture-by-technique/3d-cell-culture)
- [細胞培養培地調製](https://www.sigmaaldrich.com/JP/ja/applications/cell-culture-and-cell-culture-analysis/cell-culture-by-technique/cell-culture-media-preparation)
- [細胞増殖・細胞維持](https://www.sigmaaldrich.com/JP/ja/applications/cell-culture-and-cell-culture-analysis/cell-culture-by-technique/cell-growth-and-maintenance)
- [トランスフェクション・遺伝子編集](https://www.sigmaaldrich.com/JP/ja/applications/cell-culture-and-cell-culture-analysis/cell-culture-by-technique/transfection-and-gene-editing)
- [製造用細胞培養](https://www.sigmaaldrich.com/JP/ja/applications/cell-culture-and-cell-culture-analysis/cell-culture-for-manufacturing)
- [細胞培養トラブルシューティング](https://www.sigmaaldrich.com/JP/ja/applications/cell-culture-and-cell-culture-analysis/cell-culture-troubleshooting)
- [有機合成化学](https://www.sigmaaldrich.com/JP/ja/applications/chemistry-and-synthesis)
- [ADC・バイオコンジュゲーション](https://www.sigmaaldrich.com/JP/ja/applications/chemistry-and-synthesis/adc-and-bioconjugation)
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- [体外診断薬用原料・組織染色試薬](https://www.sigmaaldrich.com/JP/ja/applications/clinical-testing-and-diagnostics-manufacturing)
- [細菌学](https://www.sigmaaldrich.com/JP/ja/applications/clinical-testing-and-diagnostics-manufacturing/bacteriology)
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- [細胞学・細胞診](https://www.sigmaaldrich.com/JP/ja/applications/clinical-testing-and-diagnostics-manufacturing/cytology-and-microscopy)
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- [体外診断薬 (IVD) 製造](https://www.sigmaaldrich.com/JP/ja/applications/clinical-testing-and-diagnostics-manufacturing/ivd-manufacturing)
- [臨床検査に適した水](https://www.sigmaaldrich.com/JP/ja/applications/water-purification/water-clinical-diagnostics)
- [環境検査・大麻関連試験](https://www.sigmaaldrich.com/JP/ja/applications/environmental-testing-and-industrial-hygiene)
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- [ATP検査](https://www.sigmaaldrich.com/JP/ja/applications/environmental-testing-and-industrial-hygiene/cannabis-testing)
- [カンナビノイド・関連類縁物質](https://www.sigmaaldrich.com/JP/ja/applications/environmental-testing-and-industrial-hygiene/cannabis-testing/cannabinoid-profiling-potency-testing)
- [農薬・マイコトキシン](https://www.sigmaaldrich.com/JP/ja/applications/environmental-testing-and-industrial-hygiene/cannabis-testing/pesticides-mycotoxins-in-cannabis)
- [テルペン](https://www.sigmaaldrich.com/JP/ja/applications/environmental-testing-and-industrial-hygiene/cannabis-testing/terpenes-cannabis)
- [飲料水試験](https://www.sigmaaldrich.com/JP/ja/applications/environmental-testing-and-industrial-hygiene/drinking-water-testing)
- [土壌・固形廃棄物・地下水検査](https://www.sigmaaldrich.com/JP/ja/applications/environmental-testing-and-industrial-hygiene/soil-solid-waste-groundwater-testing)
- [廃水・工程用水の検査](https://www.sigmaaldrich.com/JP/ja/applications/environmental-testing-and-industrial-hygiene/waste-water-and-process-water-testing)
- [食品/飲料の検査・製造](https://www.sigmaaldrich.com/JP/ja/applications/food-and-beverage-testing-and-manufacturing)
- [化学分析](https://www.sigmaaldrich.com/JP/ja/applications/food-and-beverage-testing-and-manufacturing/chemical-analysis-for-food-and-beverage)
- [香料調合](https://www.sigmaaldrich.com/JP/ja/applications/food-and-beverage-testing-and-manufacturing/flavor-and-fragrance-formulation)
- [微生物学的分析](https://www.sigmaaldrich.com/JP/ja/applications/food-and-beverage-testing-and-manufacturing/microbiological-analysis-for-food-and-beverage)
- [法規制の順守](https://www.sigmaaldrich.com/JP/ja/applications/food-and-beverage-testing-and-manufacturing/regulatory-compliance-for-food-and-beverage)
- [ゲノミクス](https://www.sigmaaldrich.com/JP/ja/applications/genomics)
- [高度な遺伝子編集](https://www.sigmaaldrich.com/JP/ja/applications/genomics/advanced-gene-editing)
- [クローニング・発現](https://www.sigmaaldrich.com/JP/ja/applications/genomics/cloning-and-expression)
- [DNA・RNAの精製](https://www.sigmaaldrich.com/JP/ja/applications/genomics/dna-and-rna-purification)
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- [遺伝子発現・遺伝子サイレンシング](https://www.sigmaaldrich.com/JP/ja/applications/genomics/gene-expression-and-silencing)
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- [核酸ゲル電気泳動法](https://www.sigmaaldrich.com/JP/ja/applications/genomics/nucleic-acid-gel-electrophoresis)
- [核酸の標識法と検出](https://www.sigmaaldrich.com/JP/ja/applications/genomics/nucleic-acid-labeling-and-detection)
- [PCR](https://www.sigmaaldrich.com/JP/ja/applications/genomics/pcr)
- [定量PCR（qPCR）](https://www.sigmaaldrich.com/JP/ja/applications/genomics/qpcr)
- [シーケンシング](https://www.sigmaaldrich.com/JP/ja/applications/genomics/sequencing)
- [材料科学・エンジニアリング](https://www.sigmaaldrich.com/JP/ja/applications/materials-science-and-engineering)
- [3Dバイオプリンティング](https://www.sigmaaldrich.com/JP/ja/applications/materials-science-and-engineering/3d-bioprinting)
- [3Dプリンティング](https://www.sigmaaldrich.com/JP/ja/applications/materials-science-and-engineering/3d-printing)
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- [バイオエレクトロニクス](https://www.sigmaaldrich.com/JP/ja/applications/materials-science-and-engineering/bioelectronics)
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- [化学気相成長 （CVD）](https://www.sigmaaldrich.com/JP/ja/applications/materials-science-and-engineering/chemical-vapor-deposition)
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- [電子顕微鏡](https://www.sigmaaldrich.com/JP/ja/applications/materials-science-and-engineering/electron-microscopy)
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- [ナノ・マイクロ粒子合成](https://www.sigmaaldrich.com/JP/ja/applications/materials-science-and-engineering/nanoparticle-and-microparticle-synthesis)
- [有機エレクトロニクス](https://www.sigmaaldrich.com/JP/ja/applications/materials-science-and-engineering/organic-electronics)
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- [高分子合成 (ポリマー合成)](https://www.sigmaaldrich.com/JP/ja/applications/materials-science-and-engineering/polymer-synthesis)
- [固相合成](https://www.sigmaaldrich.com/JP/ja/applications/materials-science-and-engineering/solid-state-synthesis)
- [組織工学](https://www.sigmaaldrich.com/JP/ja/applications/materials-science-and-engineering/tissue-engineering)
- [微生物検査](https://www.sigmaaldrich.com/JP/ja/applications/microbiological-testing)
- [バイオバーデン試験](https://www.sigmaaldrich.com/JP/ja/applications/microbiological-testing/bioburden-testing)
- [微生物試験用の培地調製](https://www.sigmaaldrich.com/JP/ja/applications/microbiological-testing/microbial-culture-media-preparation)
- [培地充てん試験 (APS)](https://www.sigmaaldrich.com/JP/ja/applications/microbiological-testing/media-fills)
- [水試験 (微生物)](https://www.sigmaaldrich.com/JP/ja/applications/microbiological-testing/microbial-water-testing)
- [マイコプラズマ否定試験](https://www.sigmaaldrich.com/JP/ja/applications/microbiological-testing/mycoplasma-testing)
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- [無菌試験](https://www.sigmaaldrich.com/JP/ja/applications/microbiological-testing/sterility-testing)
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- [エアーモニタリング (微生物)](https://www.sigmaaldrich.com/JP/ja/applications/microbiological-testing/viable-air-monitoring)
- [モノクローナル抗体の開発・製造](https://www.sigmaaldrich.com/JP/ja/integrated-offerings/monoclonal-antibodies)
- [抗体創薬](https://www.sigmaaldrich.com/JP/ja/integrated-offerings/monoclonal-antibodies/antibody-drug-discovery)
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- [ペプチドマッピング](https://www.sigmaaldrich.com/JP/ja/integrated-offerings/monoclonal-antibodies/characterization-and-quality-control/peptide-mapping)
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- [力価測定](https://www.sigmaaldrich.com/JP/ja/integrated-offerings/monoclonal-antibodies/characterization-and-quality-control/titer-determination)
- [モノクローナル抗体の開発と製造](https://www.sigmaaldrich.com/JP/ja/integrated-offerings/monoclonal-antibodies/process-development-manufacturing)
- [mAbダウンストリーム](https://www.sigmaaldrich.com/JP/ja/integrated-offerings/monoclonal-antibodies/process-development-manufacturing/downstream-processing-mabs)
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- [mRNA医薬品の開発・製造](https://www.sigmaaldrich.com/JP/ja/integrated-offerings/mrna-development-manufacturing)
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- [mRNA製造・製剤化](https://www.sigmaaldrich.com/JP/ja/integrated-offerings/mrna-development-manufacturing/mrna-manufacturing-formulation)
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- [医薬品・バイオ医薬品製造](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing)
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- [コンジュゲーション](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/adc-manufacturing/conjugation)
- [Biopharma 4.0](https://www.sigmaaldrich.com/JP/ja/integrated-offerings/biopharma-4-0)
- [バイオプロセスの自動化・制御ソフトウェア](https://www.sigmaaldrich.com/JP/ja/integrated-offerings/biopharma-4-0/bioprocessing-automation-control-software)
- [バイオプロセスのデータ解析ソフトウェア](https://www.sigmaaldrich.com/JP/ja/integrated-offerings/biopharma-4-0/bioprocessing-data-analytics-software)
- [バイオ製造のための閉鎖系プロセス](https://www.sigmaaldrich.com/JP/ja/integrated-offerings/biopharma-4-0/closed-processing-for-biomanufacturing)
- [アップストリームプロセス（USP）の強化・連続化](https://www.sigmaaldrich.com/JP/ja/integrated-offerings/biopharma-4-0/intensified-upstream-processing)
- [ダウンストリームプロセスの強化・連続プロセス](https://www.sigmaaldrich.com/JP/ja/integrated-offerings/biopharma-4-0/intensified-continuous-downstream-processing)
- [プロセス分析技術（PAT)](https://www.sigmaaldrich.com/JP/ja/integrated-offerings/biopharma-4-0/process-analytical-technology)
- [バイオテクノロジーリソースハブ](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/biotech-resource-hub)
- [創薬](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/biotech-resource-hub/discovery)
- [前臨床](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/biotech-resource-hub/pre-clinical)
- [第Ⅰ/Ⅱ相](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/biotech-resource-hub/phase-i-ii)
- [第III相・製造](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/biotech-resource-hub/phase-iii-and-manufacturing)
- [規制](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/biotech-resource-hub/regulatory)
- [スタートアッププログラム](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/biotech-resource-hub/startup-programs)
- [血漿分画製剤](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/plasma-manufacturing)
- [細胞治療製品の製造](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/cell-therapy-manufacturing)
- [低分子医薬品の製造](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/classical-pharma-manufacturing)
- [最終充填システム](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/classical-pharma-manufacturing/final-fill-strategies)
- [液体製剤ストラテジー](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/classical-pharma-manufacturing/liquid-formulation-strategies)
- [固形製剤ストラテジー](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/classical-pharma-manufacturing/solid-formulation-strategies)
- [無菌ろ過ストラテジー](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/classical-pharma-manufacturing/sterile-filtration-strategies)
- [遺伝子治療マニファクチャリング](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/gene-therapy-manufacturing)
- [ウイルスベクターアップストリームプロセス](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/gene-therapy-manufacturing/viral-vector-upstream-processing)
- [ウイルスベクターダウンストリームプロセス](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/gene-therapy-manufacturing/viral-vector-downstream-processing)
- [ウイルスベクター製剤・最終充填](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/gene-therapy-manufacturing/viral-vector-formulation-and-final-fill)
- [ウイルスベクターの特性評価・バイオセーフティー試験](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/gene-therapy-manufacturing/viral-vector-characterization-and-biosafety-testing)
- [ウイルスベクター開発・製造受託](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/gene-therapy-manufacturing/viral-vector-contract-development-and-manufacturing)
- [Pharma Analysis & QC](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/pharmaceutical-analysis-quality-control)
- [バイオ医薬品・バイオシミラーの特性評価](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/pharmaceutical-analysis-quality-control/biologics-biosimilars-characterization)
- [局方試験適合・規制適合ガイダンス](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/pharmaceutical-analysis-quality-control/compendial-testing-regulatory-guidance)
- [低分子試験と品質管理](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/pharmaceutical-analysis-quality-control/small-molecules-analysis-quality-control)
- [滅菌・無菌サンプリング](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/sampling)
- [ワクチン製造](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/vaccine-manufacturing)
- [細胞ベースのワクチン製造](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/vaccine-manufacturing/cell-based-vaccine-manufacturing)
- [mRNAワクチン製造](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/vaccine-manufacturing/mrna-vaccines-process-manufacturing)
- [pDNAワクチン製造](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/vaccine-manufacturing/plasmid-dna-vaccines-manufacturing)
- [タンパク質サブユニットワクチンの製造](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/vaccine-manufacturing/protein-subunit-vaccine-manufacturing)
- [ウィルス様粒子 (VLP) ワクチン製造](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/vaccine-manufacturing/viral-like-particles-vaccine-manufacturing)
- [ウィルスベクターワクチン製造](https://www.sigmaaldrich.com/JP/ja/applications/pharmaceutical-and-biopharmaceutical-manufacturing/vaccine-manufacturing/viral-vector-vaccine-manufacturing)
- [タンパク質生物学](https://www.sigmaaldrich.com/JP/ja/applications/protein-biology)
- [ELISA（酵素結合免疫吸着測定法）](https://www.sigmaaldrich.com/JP/ja/applications/protein-biology/elisa)
- [酵素活性アッセイ](https://www.sigmaaldrich.com/JP/ja/applications/protein-biology/enzyme-activity-assays)
- [フローサイトメトリー](https://www.sigmaaldrich.com/JP/ja/applications/protein-biology/flow-cytometry)
- [ゲル電気泳動](https://www.sigmaaldrich.com/JP/ja/applications/protein-biology/gel-electrophoresis)
- [免疫組織染色](https://www.sigmaaldrich.com/JP/ja/applications/protein-biology/immunohistochemistry)
- [タンパク質経路の解析](https://www.sigmaaldrich.com/JP/ja/applications/protein-biology/interrogation-protein-pathways)
- [溶解・タンパク質抽出](https://www.sigmaaldrich.com/JP/ja/applications/protein-biology/lysis-and-protein-extraction)
- [タンパク質・核酸の相互作用](https://www.sigmaaldrich.com/JP/ja/applications/protein-biology/protein-and-nucleic-acid-interactions)
- [タンパク質濃縮・バッファー交換](https://www.sigmaaldrich.com/JP/ja/applications/protein-biology/protein-concentration-and-buffer-exchange)
- [タンパク質発現](https://www.sigmaaldrich.com/JP/ja/applications/protein-biology/protein-expression)
- [タンパク質標識・修飾](https://www.sigmaaldrich.com/JP/ja/applications/protein-biology/protein-labeling-and-modification)
- [タンパク質プルダウンアッセイ](https://www.sigmaaldrich.com/JP/ja/applications/protein-biology/protein-pulldown)
- [タンパク質精製](https://www.sigmaaldrich.com/JP/ja/applications/protein-biology/protein-purification)
- [タンパク質定量](https://www.sigmaaldrich.com/JP/ja/applications/protein-biology/protein-quantitation)
- [タンパク質構造分析](https://www.sigmaaldrich.com/JP/ja/applications/protein-biology/protein-structural-analysis)
- [ウェスタンブロッティング](https://www.sigmaaldrich.com/JP/ja/applications/protein-biology/western-blotting)
- [タンパク質質量分析](https://www.sigmaaldrich.com/JP/ja/applications/protein-biology/protein-mass-spectrometry)
- [研究・疾患領域](https://www.sigmaaldrich.com/JP/ja/applications/research-disease-areas)
- [がん研究](https://www.sigmaaldrich.com/JP/ja/applications/research-disease-areas/cancer-research)
- [細胞・発生生物学研究](https://www.sigmaaldrich.com/JP/ja/applications/research-disease-areas/cell-and-developmental-biology-research)
- [細胞シグナル伝達](https://www.sigmaaldrich.com/JP/ja/applications/research-disease-areas/cell-signaling)
- [糖鎖生物学](https://www.sigmaaldrich.com/JP/ja/applications/research-disease-areas/glycobiology)
- [メタボロミクス研究](https://www.sigmaaldrich.com/JP/ja/applications/research-disease-areas/metabolomics-research)
- [神経科学研究](https://www.sigmaaldrich.com/JP/ja/applications/research-disease-areas/neuroscience-research)
- [薬理学・創薬研究](https://www.sigmaaldrich.com/JP/ja/applications/research-disease-areas/pharmacology-and-drug-discovery-research)
- [超純水・純水製造装置](https://www.sigmaaldrich.com/JP/ja/applications/water-purification)
- [化学分析に適した水](https://www.sigmaaldrich.com/JP/ja/applications/water-purification/water-analytical-chemistry)
- [細胞・分子生物学に適した水](https://www.sigmaaldrich.com/JP/ja/applications/water-purification/water-cell-molecular-biology)
- [臨床検査に適した水](https://www.sigmaaldrich.com/JP/ja/applications/water-purification/water-clinical-diagnostics)
- サービス
- [受託製造](https://www.sigmaaldrich.com/JP/ja/services/contract-manufacturing)
- [ポリエチレングリコール（PEG）](https://www.sigmaaldrich.com/JP/ja/services/contract-manufacturing/peg)
- [体外診断薬用OEM・CMOサービス](https://www.sigmaaldrich.com/JP/ja/services/ivd-development-manufacturing/diagnostic-oem-cmo)
- [ADC・バイオコンジュゲーション](https://www.sigmaaldrich.com/JP/ja/services/contract-manufacturing/adc-bioconjugation)
- [セルバンク製造](https://www.sigmaaldrich.com/JP/ja/services/contract-manufacturing/cell-bank-manufacturing)
- [複合API](https://www.sigmaaldrich.com/JP/ja/services/contract-manufacturing/complex-apis)
- [高薬理活性医薬原体](https://www.sigmaaldrich.com/JP/ja/services/contract-manufacturing/high-potent-apis)
- [哺乳動物由来バイオ医薬品](https://www.sigmaaldrich.com/JP/ja/services/contract-manufacturing/mab-manufacturing)
- [mRNA・LNPの製剤化](https://www.sigmaaldrich.com/JP/ja/services/contract-manufacturing/mrna-and-lnp-formulation-ctdmo-services)
- [脂質ナノ粒子製剤 CDMOサービス](https://www.sigmaaldrich.com/JP/ja/services/contract-manufacturing/mrna-and-lnp-formulation-ctdmo-services/lipid-nanoparticle-cdmo-services)
- [ウイルスベクター製造](https://www.sigmaaldrich.com/JP/ja/services/contract-manufacturing/viral-vector-manufacturing)
- [遺伝子治療プロセス開発](https://www.sigmaaldrich.com/JP/ja/services/contract-manufacturing/viral-vector-manufacturing/gene-therapy-process-development)
- [レンチウイルスベクター](https://www.sigmaaldrich.com/JP/ja/services/contract-manufacturing/viral-vector-manufacturing/lentiviral-vectors)
- [バルク・カスタム製品](https://www.sigmaaldrich.com/JP/ja/services/custom-products)
- [バルク・特注合成サービス](https://www.sigmaaldrich.com/JP/ja/services/custom-products/bulk-and-custom-services)
- [秤量済み原料搬送](https://www.sigmaaldrich.com/JP/ja/services/custom-products/bulk-powder-transfer-bags)
- [細胞培養培地の安定性・試験サービス](https://www.sigmaaldrich.com/JP/ja/services/custom-products/cell-culture-media-stability-and-testing-services)
- [Cell Design Studio™ セルエンジニアリングサービス](https://www.sigmaaldrich.com/JP/ja/services/custom-products/custom-cell-lines)
- [CHOZN<sup>®</sup>細胞株の設計・開発](https://www.sigmaaldrich.com/JP/ja/services/custom-products/chozn-cell-line-engineering-and-development)
- [受託ZooMAb<sup>®</sup>抗体開発サービス](https://www.sigmaaldrich.com/JP/ja/services/custom-products/contract-zoomab-antibody-development-services)
- [医薬品製造原料（SAFCブランド製品）](https://www.sigmaaldrich.com/JP/ja/services/custom-products/critical-raw-materials)
- [カスタム診断薬用抗体の開発・製造サービス](https://www.sigmaaldrich.com/JP/ja/services/ivd-development-manufacturing/custom-diagnostic-antibody-development-and-manufacturing-services)
- [カスタムGCカラム](https://www.sigmaaldrich.com/JP/ja/services/custom-products/customized-gc-columns)
- [カスタムGMP細胞培養培地](https://www.sigmaaldrich.com/JP/ja/services/custom-products/custom-gmp-cell-culture-products)
- [カスタム (特注品) HPLCカラム](https://www.sigmaaldrich.com/JP/ja/services/custom-products/custom-hplc-column-request)
- [カスタム培地開発サービス](https://www.sigmaaldrich.com/JP/ja/services/custom-products/custom-media-development-services)
- [カスタムサービス：MILLIPLEX<sup>®</sup>マルチプレックスアッセイ・ ELISA](https://www.sigmaaldrich.com/JP/ja/services/custom-products/custom-milliplex-multiplex-assays-and-elisas)
- [受託モノクローナル抗体作製](https://www.sigmaaldrich.com/JP/ja/services/custom-products/custom-monoclonal-antibody)
- [受託DNA免疫法ラット/マウス・モノクローナル抗体作製](https://www.sigmaaldrich.com/JP/ja/services/custom-products/custom-monoclonal-antibody/custom-dna-immunoprescription-rat-mouse-monoclonal-antibody)
- [受託ウサギリコンビナント・モノクローナル抗体作製](https://www.sigmaaldrich.com/JP/ja/services/custom-products/custom-monoclonal-antibody/custom-rabbit-recombinant-monoclonal-antibody)
- [受託ラット/マウス・モノクローナル抗体作製](https://www.sigmaaldrich.com/JP/ja/services/custom-products/custom-monoclonal-antibody/custom-rat-mouse-monoclonal-antibody)
- [カスタム核酸](https://www.sigmaaldrich.com/JP/ja/products/molecular-biology-and-functional-genomics/oligos-and-qpcr-probes)
- [カスタムSPEチューブ・96ウェルプレート](https://www.sigmaaldrich.com/JP/ja/services/custom-products/custom-spe-tubes-and-96-well-plates)
- [カスタムSPMEファイバー](https://www.sigmaaldrich.com/JP/ja/services/custom-products/custom-spme-fibers)
- [カスタムテストストリップ・試験紙](https://www.sigmaaldrich.com/JP/ja/services/custom-products/custom-test-strips-and-papers)
- [体外診断薬用OEM・CMOサービス](https://www.sigmaaldrich.com/JP/ja/services/ivd-development-manufacturing/diagnostic-oem-cmo)
- [Eshmuno<sup>®</sup> Fitカスタムアフィニティーレジン開発サービス](https://www.sigmaaldrich.com/JP/ja/services/custom-products/eshmuno-fit-custom-affinity-resin-development-services)
- [標準物質](https://www.sigmaaldrich.com/JP/ja/services/custom-products/reference-materials)
- [低分子ライブラリーデザイン (Aldrich Market Select)](https://www.sigmaaldrich.com/JP/ja/services/custom-products/small-molecule-library-design)
- [安定同位体カスタム・GMP製品](https://www.sigmaaldrich.com/JP/ja/services/custom-products/stable-isotope-custom-and-gmp-products)
- [デジタルプラットフォーム・ソフトウェア](https://www.sigmaaldrich.com/JP/ja/services/software-and-digital-platforms)
- [AIDDISON™ AI-Powered Drug Discovery Software](https://www.sigmaaldrich.com/JP/ja/services/software-and-digital-platforms/aiddison-ai-powered-drug-discovery)
- [Belysa<sup>®</sup> Immunoassay Curve Fitting Software](https://www.sigmaaldrich.com/JP/ja/services/software-and-digital-platforms/belysa-immunoassay-curve-fitting-software)
- [ChemisTwin™ Portal](https://www.sigmaaldrich.com/JP/ja/services/software-and-digital-platforms/digital-reference-materials)
- [DELデータ拡張サービス](https://www.sigmaaldrich.com/JP/ja/services/software-and-digital-platforms/dna-encoded-library-technology-drug-discovery)
- [DOZN™ツール](https://www.sigmaaldrich.com/JP/ja/services/software-and-digital-platforms/dozn-tool)
- [LANEXO<sup>®</sup>システム](https://www.sigmaaldrich.com/JP/ja/services/software-and-digital-platforms/lanexo-inventory-manager)
- [Millicell<sup>®</sup>クラウドサービス](https://www.sigmaaldrich.com/JP/ja/services/software-and-digital-platforms/millicell-cloud-services)
- [MQuant<sup>®</sup> StripScan](https://www.sigmaaldrich.com/JP/ja/services/software-and-digital-platforms/mquant-stripscan)
- [MyMilli-Q<sup>™</sup>デジタルサービス](https://www.sigmaaldrich.com/JP/ja/services/software-and-digital-platforms/mymilli-q-digital-services)
- [SYNTHIA<sup>™</sup>逆合成解析ソフトウエア](https://www.sigmaaldrich.com/JP/ja/services/software-and-digital-platforms/synthia-retrosynthesis-software)
- [Val@M™アプリケーションによるバリデーション作業](https://www.sigmaaldrich.com/JP/ja/services/software-and-digital-platforms/valatm-validation-platform)
- [M-Trace<sup>®</sup>試験の電子記録ソフトウェア](https://www.sigmaaldrich.com/JP/ja/services/software-and-digital-platforms/m-trace-electronic-test-record-software)
- [診断薬の開発・製造](https://www.sigmaaldrich.com/JP/ja/services/ivd-development-manufacturing)
- [カスタム診断薬用抗体の開発・製造サービス](https://www.sigmaaldrich.com/JP/ja/services/ivd-development-manufacturing/custom-diagnostic-antibody-development-and-manufacturing-services)
- [体外診断薬用OEM・CMOサービス](https://www.sigmaaldrich.com/JP/ja/services/ivd-development-manufacturing/diagnostic-oem-cmo)
- [ラテラルフローアッセイ開発サービス](https://www.sigmaaldrich.com/JP/ja/services/ivd-development-manufacturing/lateral-flow-services)
- [mRNA医薬品の開発・製造](https://www.sigmaaldrich.com/JP/ja/integrated-offerings/mrna-development-manufacturing)
- [mRNA医薬品の分析・品質管理](https://www.sigmaaldrich.com/JP/ja/integrated-offerings/mrna-development-manufacturing/mrna-analytical-quality-control)
- [mRNA製造工程開発](https://www.sigmaaldrich.com/JP/ja/integrated-offerings/mrna-development-manufacturing/mrna-development)
- [mRNA製造・製剤化](https://www.sigmaaldrich.com/JP/ja/integrated-offerings/mrna-development-manufacturing/mrna-manufacturing-formulation)
- [mRNA研究・開発](https://www.sigmaaldrich.com/JP/ja/integrated-offerings/mrna-development-manufacturing/mrna-research)
- [製品サービス](https://www.sigmaaldrich.com/JP/ja/services/product-services)
- [固形製剤アプリケーションサービス](https://www.sigmaaldrich.com/JP/ja/services/product-services/application-services-solid-dose-formulations)
- [装置の校正・点検](https://www.sigmaaldrich.com/JP/ja/services/product-services/calibration-and-verification)
- [Milli-Q <sup>®</sup>キャリブレーション・ベリフィケーション](https://www.sigmaaldrich.com/JP/ja/services/product-services/calibration-and-verification/calibration-verification-suitability-testing-of-milli-q-water-pu)
- [微生物検査装置](https://www.sigmaaldrich.com/JP/ja/services/product-services/calibration-and-verification/calibration-verification-microbiology)
- [Elevateプログラム](https://www.sigmaaldrich.com/JP/ja/services/product-services/elevate-quality-program)
- [eMERGE®プログラム](https://www.sigmaaldrich.com/JP/ja/services/product-services/the-emerge-standard-edata-exchange-platform)
- [Emprove<sup>®</sup>プログラム](https://www.sigmaaldrich.com/JP/ja/services/product-services/emprove-program)
- [Emprove<sup>®</sup>細胞培養用培地](https://www.sigmaaldrich.com/JP/ja/services/product-services/emprove-program/emprove-cell-culture-media)
- [Emprove<sup>®</sup>医薬品原材料ポートフォリオ](https://www.sigmaaldrich.com/JP/ja/services/product-services/emprove-program/emprove-chemicals-portfolio)
- [Emprove<sup>®</sup>クロマトグラフィーポートフォリオ](https://www.sigmaaldrich.com/JP/ja/services/product-services/emprove-program/emprove-chromatography-portfolio)
- [Emprove<sup>®</sup>フィルター・シングルユース製品ポートフォリオ](https://www.sigmaaldrich.com/JP/ja/services/product-services/emprove-program/emprove-filter-and-single-use-component-portfolio)
- [Emprove<sup>®</sup> Suite](https://www.sigmaaldrich.com/JP/ja/services/product-services/emprove-program/emprove-suite)
- [メンテナンス・サービスプラン](https://www.sigmaaldrich.com/JP/ja/services/product-services/maintenance-and-service-plans)
- [微生物検査システム](https://www.sigmaaldrich.com/JP/ja/services/product-services/maintenance-and-service-plans/microbiology-maintenance-and-service-plans)
- [Milli-Q<sup>®</sup> メンテナンス・サービスプラン](https://www.sigmaaldrich.com/JP/ja/services/product-services/maintenance-and-service-plans/milli-q-services-plans-and-maintenance-for-water-purification-sy)
- [Spectroquant <sup>®</sup>光度計サービス](https://www.sigmaaldrich.com/JP/ja/services/product-services/systems-qualification/spectroquant-photometer-services)
- [システムサービスリライアンスプラン](https://www.sigmaaldrich.com/JP/ja/services/product-services/maintenance-and-service-plans/system-service-reliance-plans)
- [Milli-Q<sup>®</sup>サービス・サポート](https://www.sigmaaldrich.com/JP/ja/services/product-services/milli-q-services-and-support)
- [メンテナンス・サービスプラン](https://www.sigmaaldrich.com/JP/ja/services/product-services/maintenance-and-service-plans/milli-q-services-plans-and-maintenance-for-water-purification-sy)
- [クオリフィケーション・バリデーション](https://www.sigmaaldrich.com/JP/ja/services/product-services/systems-qualification/milliq-qualification-program)
- [装置の校正・点検](https://www.sigmaaldrich.com/JP/ja/services/product-services/calibration-and-verification/calibration-verification-suitability-testing-of-milli-q-water-pu)
- [その他サービス](https://www.sigmaaldrich.com/JP/ja/services/product-services/milli-q-services-and-support/milli-q-additional-services)
- [MyMilli-Q™デジタルサービス](https://www.sigmaaldrich.com/JP/ja/services/software-and-digital-platforms/mymilli-q-digital-services)
- [シングルユース・フィルターバリデーションサービス](https://www.sigmaaldrich.com/JP/ja/services/product-services/single-use-filter-validation)
- [バクテリアチャレンジ試験](https://www.sigmaaldrich.com/JP/ja/services/product-services/single-use-filter-validation/bacterial-retention)
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- [抽出物・浸出物](https://www.sigmaaldrich.com/JP/ja/services/product-services/single-use-filter-validation/extractables-and-leachables)
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