[コンテンツへスキップ](https://www.sigmaaldrich.com#main-content) [![Merck](https://www.sigmaaldrich.com/static/logos/purple/merck.svg)](https://www.sigmaaldrich.com/JP/ja) 製品 カート0 JPJA 製品 [ログイン / 登録](https://www.sigmaaldrich.com/oidc-sign-in) [注文検索](https://www.sigmaaldrich.com/JP/ja/order-lookup) [クイックオーダー](https://www.sigmaaldrich.com/JP/ja/quick-order) カート0 [Home](https://www.sigmaaldrich.com/JP/en)[Sample Purification](https://www.sigmaaldrich.com/JP/en/applications/analytical-chemistry/sample-preparation/purification)Selection of Purification Equipment # Selection of Purification Equipment Simple IEX chromatography, such as elution by a step-gradient, can be performed using a syringe or peristaltic pump with prepacked HiTrap columns. A chromatography system is required in order to achieve a high resolution separation using accurately controlled linear gradient elution, to take advantage of the high flow rates of modern media, or when the same column is to be used for many runs. Way of workingPilotExplorer 100Purifier 10FPLCPrimeSyringe or peristaltic pump + HiTrap column Simple, step-gradient elution✓✓✓✓✓✓ Reproducible performance for routine separation✓✓✓✓✓ Optimization of one step separation to increase purity✓✓✓✓✓ System control and data handling for regulatory requirements, e.g. GLP✓✓✓✓ Automatic method development and optimization✓✓✓✓ Automatic buffer preparation✓✓✓ Automatic pH scouting✓✓✓ Automatic media or column scouting✓✓ Automatic multi-step purification✓✓ Scale up, process development and transfer to production✓✓ Proven sanitary design for cGMP with easily exchanged wetted parts✓ Standard ÄKTAdesign configurations ![Standard ÄKTAdesign configurations](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/analytical-chemistry/purification/akt-design-configurations.jpg "Standard ÄKTAdesign configurations") __Figure 1.__ Standard ÄKTAdesign configurations ## Materials 申し訳ございませんが、想定外のエラーが発生しました。 Response not successful: Received status code 500 __Related Articles__ - [Maintenance of Multimodal Chromatography Media and Storage Conditions](https://www.sigmaaldrich.com/JP/en/technical-documents/protocol/protein-biology/protein-purification/maintenance-of-media-and-storage-conditions) - [Bind/Elute vs Flowthrough Mode in Multimodal Chromatography](https://www.sigmaaldrich.com/JP/en/technical-documents/technical-article/analytical-chemistry/purification/bind-elute-vs-flowthrough-mode-in-multimodal-chromatography) - [Chiral Chromatography Frequently Asked Questions](https://www.sigmaaldrich.com/JP/en/technical-documents/technical-article/analytical-chemistry/purification/faq) - [FastWoRX for Faster and Greener Alternative to Liquid-Liquid Extraction](https://www.sigmaaldrich.com/JP/en/technical-documents/technical-article/analytical-chemistry/purification/fastworx) - [High Purity Carbon Adsorbents for Chromatography](https://www.sigmaaldrich.com/JP/en/technical-documents/technical-article/analytical-chemistry/purification/high-purity-carbon-adsorbents) - [Plasmid purification by ion exchange chromatography with Genopure kits](https://www.sigmaaldrich.com/JP/en/technical-documents/technical-article/analytical-chemistry/purification/ion-exchange-chromatography) - [LRA (Lipid Removal Agent) for Biopharmaceutical Production](https://www.sigmaaldrich.com/JP/en/technical-documents/technical-article/analytical-chemistry/purification/lra-lipid-removal) - [Immobilized Metal Chelate Affinity Chromatography (IMAC)](https://www.sigmaaldrich.com/JP/en/technical-documents/technical-article/analytical-chemistry/purification/metal-chelate-afnity-chromatography) - [View More](https://www.sigmaaldrich.com/JP/en/search/facet-search?focus=sitecontent&term=facet-search) TOP __ログインして続行__ 続きを確認するには、ログインするか、新規登録が必要です。 ログイン__アカウントをお持ちではありませんか？__お客様情報を登録 An unknown error has occured. - 日本語 - JA - English - EN [詳細はこちら](https://www.sigmaaldrich.com)