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Merck

I6529

SAFC

IMDM

With sodium bicarbonate and ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Iscove′s MDM, Iscoves MDM, Iscove’s Modified Dulbecco’s Medium, IMDM

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500 ML
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₩64,757

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About This Item

MDL number:
MFCD00217469
UNSPSC Code:
12352207
NACRES:
NA.75

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Product Name

Iscove′s Modified Dulbecco′s Medium, With sodium bicarbonate and L-glutamine, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: yes
phenol red: yes
NaHCO3: yes
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

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This Item
I7633I339051471C
SAFC

SAFC

I6529

Iscove′s Modified Dulbecco′s Medium

Iscove’s Modified Dulbecco’s Medium With L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

Sigma-Aldrich

I7633

Iscove′s Modified Dulbecco′s Medium

Sigma-Aldrich

Sigma-Aldrich

I3390

Iscove′s Modified Dulbecco′s Medium

Iscove’s Modified Dulbecco’s Medium liquid

SAFC

51471C

Iscove′s Modified Dulbecco′s Medium

form

liquid

form

powder

form

liquid

form

liquid

sterility

sterile-filtered

sterility

-

sterility

sterile-filtered

sterility

sterile

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable, cell culture | stem cell: suitable

components

glucose: yes
phenol red: yes
NaHCO3: yes
L-glutamine: yes

components

phenol red: yes
glucose: yes
L-glutamine: yes

components

phenol red: yes
L-glutamine: no
glucose: yes

components

phenol red: 15.93 mg/L
NaHCO3: 3024 mg/L
L-glutamine: 582 mg/L
HEPES: 5958 mg/L
sodium pyruvate: 110 mg/L

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Iscove′s Modified Dulbecco′s Medium contains selenium, additional amino acids and vitamins, sodium pyruvate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, and potassium nitrate instead of ferric nitrate, and is a modification of Dulbecco′s Modified Eagle′s medium (DME). Iscove′s medium can be used to support murine B lymphocytes, bone marrow hemopoietic tissue, B cells stimulated with lipopolysaccharide, T lymphocytes, and various hybrid cells.

Application

Iscove′s Modified Dulbecco′s Medium has been used to culture:
  • human hepatocellular carcinoma (HCC) cell line HepG2 as monolayers[1]
  • to culture synovial tissue samples[2]
  • bacterial strain inoculum for stimulation of immune cell experiments[3]

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
RNBL7701
RNBL6003
RNBL2060
RNBL0515
RNBK8781

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Ram C Shankaraiah et al.
Oncogene, 38(45), 7035-7045 (2019-08-15)
Metformin is a hypoglycaemic agent used to treat type 2 diabetes mellitus (DM2) patients, with a broad safety profile. Since previous epidemiological studies had shown that the incidence of hepatocellular carcinoma (HCC) decreased significantly in metformin treated DM2 patients, we
Patricia López et al.
PloS one, 6(9), e24776-e24776 (2011-10-04)
In this work we analyzed the immune activation properties of different Bifidobacterium strains in order to establish their ability as inductors of specific effector (Th) or regulatory (Treg) responses. First, we determined the cytokine pattern induced by 21 Bifidobacterium strains
Ronna Hertzano et al.
The Laryngoscope, 131 Suppl 5, S1-S16 (2020-06-25)
The cellular diversity of the inner ear has presented a technical challenge in obtaining molecular insight into its development and function. The application of technological advancements in cell type-specific expression enable clinicians and researchers to leap forward from classic genetics
Christine Bourgeois et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(25), 8691-8696 (2008-06-20)
A model of chemical thymectomy by inducible Rag ablation was used to study peripheral T cell homeostasis. Induction of Rag ablation was efficient and complete, leading to cessation of thymic T cell production within 3-4 weeks. The decay of peripheral
Gustav Nilsonne et al.
Behavioral and brain functions : BBF, 7, 47-47 (2011-11-22)
Recent advances in cell biology and gene regulation suggest mechanisms whereby associative learning could be performed by single cells. Therefore, we explored a model of classical conditioning in human macrophages in vitro. In macrophage cultures, bacterial lipopolysaccharide (LPS; unconditioned stimulus)
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