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M6320

Sigma-Aldrich

Monoclonal Anti-MAGE-1 antibody produced in mouse

~2 mg/mL, clone MA454, purified immunoglobulin, buffered aqueous solution

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Synonym(s):
Anti-Melanoma-associatiated antigen 1
MDL number:
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MA454, monoclonal

form

buffered aqueous solution

mol wt

antigen ~46 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

concentration

~2 mg/mL

technique(s)

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 5-10 μg/mL using total cell extract of SKML37 cells

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MAGEA1(4100)

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SAB5500139N2038WH0004103M1
clone

MA454, monoclonal

clone

SP188, monoclonal

clone

E978, monoclonal

clone

3D12, monoclonal

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

Gene Information

human ... MAGEA1(4100)

Gene Information

human ... MAGEA1(4100)

Gene Information

human ... CTAG1B(1485)

Gene Information

human ... MAGEA4(4103)

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

mouse

biological source

rabbit

biological source

mouse

biological source

mouse

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General description

Melanoma-associatiated antigen 1 (MAGE-1) is part of the CG (cancer-germline) group of genes and is a tumor-specific antigen. MAGE-1 is a member of the MAGE-A family. MAGE-1 gene is localized on human chromosome Xq28. Monoclonal Anti-MAGE-1 (mouse IgG1 isotype) is derived from the hybridoma MA454 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from mice immunized with human recombinant MAGE-1.

Specificity

The antibody does not recognize MAGE-2 and MAGE-3.

Immunogen

recombinant human MAGE-1.

Application

Monoclonal Anti-MAGE-1 antibody produced in mouse has been used in:
  • ELISA
  • immunoblotting
  • immunohistochemistry

Biochem/physiol Actions

The melanoma antigen genes (MAGE) might play a key role in neural development and apoptosis. Expression of melanoma-associated antigen 1 (MAGE-1) in neoplastic cells depends on the binding of the transcription factor Ets to non-methylated cis regulatory sequences, located in its promoter. MAGE-1 expression is mediatedby the B and B′ domains of MAGE-A1 gene. Expression of MAGE-1A was induced in non MAGE-A1 expressing tumor cells once treated with demethylating reagent, 5-aza-2-(5DC). Melanoma-associatiated antigen 1 (MAGE-1) has been shown to be expressed in different types of cancers.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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The MAGE Proteins: Emerging Roles in Cell
Cycle Progression, Apoptosis, and
Neurogenetic Disease
Barker PA and Salehi A
Journal of Neuroscience Research (2002)
Jiang Xiao et al.
World journal of gastroenterology, 10(13), 1849-1853 (2004-06-29)
To date, dozens of melanoma-associated antigens (MAGEs) have been identified and classified into 2 subgroups, I and II. Subgroup I consists of antigens which expression is generally restricted to tumor or germ cells, also named as cancer/testis (CT) antigen. Proteins
Clinical Significance of Melanoma Antigen-Encoding Gene-1 (MAGE-1) Expression and its Correlation with Poor Prognosis in Differentiated Advanced Gastric Cancer
Ogata MDK, et al.
Annals of Surgical Oncology (2011)
Madhav V Dhodapkar et al.
Cancer immunity, 3, 9-9 (2003-07-24)
Cancer/testis (CT) antigens are expressed in several malignant tumors, but not in normal tissues except for testicular germ cells. The expression of CT antigenic proteins in malignant gammopathies has not been characterized. We examined the expression of a panel of
Kyoichi Ogata et al.
Annals of surgical oncology, 18(4), 1195-1203 (2010-11-03)
Melanoma antigen-encoding gene-1 (MAGE-1), a cancer/testis antigen, has been reported to be expressed in various types of cancer. We investigated the clinicopathological features and prognostic significance of MAGE-1 expression in advanced gastric cancer (AGC). Immunohistochemical staining for MAGE-1 was performed

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