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lichropur® bases for hplc and lc-ms
Keyword:'lichropur® bases for hplc and lc-ms'
Showing 1-30 of 279 results for "lichropur® bases for hplc and lc-ms" within Technical Documents
App Note: Simplified LC-MS/MS Method for Glyphosate, AMPA, and Glufosinate in oat-based cereals
Methanol for UHPLC-MS LiChrosolv® 1.03726
Acetonitrile for UHPLC-MS LiChrosolv® 1.03725
Ammonia solution 25%, for HPLC LiChropur™ 5.43830
Formic acid for LC-MS LiChropur™, 97.5-98.5% (T) 00940
LC-MS Analysis of 33 PFAS Compounds in 5 minutes
8327 was completed in 5 minutes in this application note.
The HPLC column of choice for PFAS analysis by LC-MS/(MS) is a C18
column based on fully porous silica particles (FPP) or on superficially
porous
App Note: Charge Variant Analysis (CVA) with Strong Cation Exchange and MS Compatible Buffers
HPLC
Proteomix® SCX-NP5 (2.1 mm x 50) mm, 5 μm Z777156
Acetonitrile, LiChrosolv® hypergrade for LC-MS 1.00029
Water, LiChrosolv® for LC-MS 1.15333
Ammonium acetate, LiChropur™ for LC-MS
Brochure: LC-MS Resource Guide
Hexafluoro-2-propanol for LC-MS LiChropur™ 10 ml, 50 ml 18127
For a complete list of LC-MS and HPLC solvents,
buffers and additives, visit SigmaAldrich.com/lc-ms
LC-MS Tips and Tricks
Mobile
Reaction Monitoring of the Esterification Progress of Benzoic Acid
99.5% 242381
Acetonitrile hypergrade for LC-MS LiChrosolv® 1.00029
Water for chromatography (LC-MS Grade) LiChrosolv® 1.15333
Acetic acid for LC-MS LiChropur™ 5.33001
Reference
1. Maki, Takao;
Brighter Analysis - Solvents & Inorganics for accurate, brilliant results (RoW)
environmental analysis HPLC requires high-performance solvents and reagents.
Classical
Analysis
http://www.merckmillipore.com/Lichropur
LC-MS
Solvents
LC-MS
Reagents
Check
LC-MS Analysis of PFAS Compounds in EPA Methods 537.1, 533 and 8327
hypergrade for LC-MS LiChrosolv® 1.06035
Water for chromatography (LC-MS grade) LiChrosolv® or ultrapure water from a Milli-Q® IQ 7 series water purification system 1.15333
Ammonium acetate LiChropur™, eluent
Brighter Analysis - Solvents & Inorganics for accurate, brilliant results
Analysis
Safety
&
Essentials
HPLC
&
LC-MS
Brighter
Analysis
LC-MSHPLC
HPLC
&
LC-MS
X
Analytix Reporter Issue 11 2022
Description Cat. No.
Ammonium formate, eluent additive for LC-MS,
LiChropur™, ≥99.0%
70221
Formic acid, for HPLC LiChropur™ 5.43804
Acetonitrile, gradient grade LiChrosolv® Reag. Ph Eur
App Note: The use of DPX HLB tips and Hamilton® robot for extraction of opioid drugs from urine
phosphate dibasic heptahydrate ACS reagent, 98.0–102.0%
5.33003 Ammonia solution 25% for LC-MS LiChropur™
P5368 Phosphate buffered saline BioPerformance Certified, pH 7.4
52989-U SUPEL™ SWIFT
Effect of Extracolumn Dispersion on Chromatographic Performance Using Narrow Bore UHPLC Columns
for MS
900667 Acetonitrile, for UHPLC, suitable for MS
G4505 Guanidine hydrochloride, ≥99% (titration), organic base and chaeotropic agent
00922 Difluoroacetic acid, for LC-MS, LiChropur™
00940
Peptide Mapping Analysis Workflow for Adalimumab
Water, LiChrosolv® for LC-MS 1153334000 Supelco®
Formic acid, LiChropur™ for LC-MS 533002 Supelco®
Accessories
Autosampler vials volume 0.3 mL 29661-U Supelco®
To place an order or receive
App Note: UHPLC-MS Bottom-Up Trastuzumab BIOshell™A160 Peptide C18
for MS 900682
Acetonitrile, for UHPLC, suitable for MS 900667
Guanidine hydrochloride, ≥99% (titration), organic base and chaeotropic agent G4505
Difluoroacetic acid, for LC-MS, LiChropur™ 00922
Flyer: Determination of Δ9-tetrahydrocannabinol (THC), cannabidiol (CBD) and their metabolites in serum Hybrid SPE
Grade) LiChrosolv®
00940 Formic acid for LC-MS LiChropur™, 97.5-98.5% (T)
791725 Citric acid anhydrous, free-flowing, Redi-Dri™, ACS
reagent, ≥99.5%
52978-U HybridSPE® DPX tip
Z717266 NuncTM 96 DeepWell
Workflow for Intact and Middle-Up Mass Analysis of Adalimumab
or LiChrosolv® UHPLC-MS
grade bottled water
ZIQ7000T0
or 1.03728
Acetonitrile for UHPLC-MS LiChrosolv® 1.03725
Trifluoroacetic acid eluent additive for LC-MS,
LiChropur®
80457
Tris(2-
Cannabis Potency Testing
Formic acid 98% - 100%, for LC-MS, LiChropur™ 5330020050
Acetonitrile with 0.1 % (v/v) formic acid for UHPLC, Sigma Aldrich 900686
Ammonium formate ≥99.0%, for LC-MS, LiChropur™ 70221
Ultrapure water
App Note: Analysis of Drugs of Abuse in Urine After Cleanup with New Supel™ Swift HLB Solid Phase Extraction 96-well Plates
methanol,
ampule of 1 mL, certified reference material, Cerilliant®
00940 Formic acid for LC-MS LiChropur™, 97.5-98.5% (T)
G8132 β-Glucuronidase from limpets (Patella vulgata) Type L-II,
lyophilized
App Note: Steroid Hormone Analysis in Serum using Supel™ Swift HLB DPX Tips
of 3 ea 53504-U
Formic acid 98-100%, LC-MS LiChropur™ 5.330002
Methanol, LC-MS LiChrosolv® 1.03726
Acetonitrile, LC-MS LiChrosolv® 1.00029
Water, LC-MS LiChrosolv® 1.15333
Suggested Consumables
Wallchart: Small Molecule HPLC Column Selection Guide
quality consumables for
the U/HPLC analysis workfl ow. Our selection
of columns, solvents, standards and sample
preparation products are designed for HPLC and
LC-MS to make your analysis quick, easy
Brochure: LC-MS Contaminants
acid in isopropanol at 0.2 mL/min for 48 min.
Overview of U/HPLC columns for LC-MS
For LC-MS, particulate HPLC columns, preferably based
on high purity Type B silica, are widely used.
1. Small particles
Low-extractable Millex Samplicity®filters: ideal for preparing samples for sensitive HPLC detectors
prior to HPLC analysis
should introduce minimal extractable impurities as
detected by a fluorescence detector. Similar to the
challenges with LC-MS, fluorescence intensity varies
based on the excitation
Ultrapure water for biomedical LC analysis
were analyzed using LC-ESI-MS-MS. High purity water was utilized and results highlight the
importance of low organic contamination to reach low levels of detection.
Denaturating HPLC is a reverse phase
Solutions for Mass Spectrometry
sensitive MS analyses, as it can help to avoid
handling based contaminations.
Water sample Intensity of reserpine
signal [cps]
LC-MS grade bottled water Competitor B 8.80 x 104
LC-MS grade
Wallchart: Chiral HPLC Column Selection Guide
retention and selectivity. Typical
acid and base concentrations are 0.01
to 1%
• Acetonitrile should be the dominant
solvent with CYCLOBOND Column in POM
• LC-MS optimization: Replace TEA
with ammonium
MultiScreen Deep Well Solvinert Filter Plate: High throughput sample preparation for the quantitation of drug compounds in serum samples
following centrifugation.
LC-MS/MS analysis: Unless otherwise indicated, LC-MS/MS analyses were
performed using a Sciex API-2000 mass spectrometer coupled with an Agilent 1100
HPLC and well plate autosampler
Poster: Rapid Sample Preparation Method for High Throughput Total Drug Analysis by LC-MS/MS
high recovery
No interfering extractable to LC-MS/MS or HPLC-UV
1000
10000
100000
1000000
10 50 100 500 1000
Analyte concentration (nM)
LC
-M
S/
M
S
ar
ea
(c
ou
nt
s)
Pesticides in Cannabis
acetonitrile based gradient
produced better separation between the pesticides and major cannabinoids than other phase
chemistries, including C18. This separation would allow a switch to waste on the LC/MS/MS
system
The Power to create better outcomes for your Research
Filter holders
Atomic Absorption / ICP
UV & IR Spectroscopy
Chromatography, HPLC, LC‐MS
Photometry
GC, GC‐MS,
GC headspace
Miscellaneous
NMR
Certipur® ICP
Standards, Certipur®
AAS
Chromatography Instrumental Techniques
TLC-MS mode, the
interface sits between the HPLC pump and the MS system, and is pretty much “plug and play”. It’s
fair to say that such systems offer the lowest entry barriers into TLC-MS, and
SBR00030 Technical Bulletin
Derivatize the SCFA based on the Derivatization
instructions under Detection by LC-MS/HPLC.
2. Dilute the derivatized SCFA mixture to several
concentrations and analyze by HPLC following LC
Conditions instructions
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