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ADCC Assay Using BioIVT Cryopreserved NK Cells

ADCC assay performance and reproducibility using ready-to-use, cryopreserved NK effector cells, A549 target cells expressing a luciferase reporter gene, and anti-EGFR antibody.

Introduction to Primary Cell Culture

Yeast plasmid selection: Utilizes auxotrophic yeast strains for plasmid selection, enabling growth on synthetic media for better yield.

Primary Human Osteoblasts and Osteoblast Growth/Differentiation Media

Primary human osteoblasts and media for the optimal growth and mineralization of human osteoblast cultures. Protocols for handling, cell passaging, media, and product use.

Primary Human Smooth Muscle Cells and Growth Media

Smooth muscle human primary cells isolated from blood vessels and hollow organs, plus smooth muscle cell growth media. Protocols on media handling, cell passaging and product use.

Mouse Embryonic Fibroblasts (MEF Feeder Cells)

Mouse embryonic fibroblasts (MEFs) serve as a feeder layer for both mouse and human embryonic stem cells (ES cells) and induced pluripotent stem cells (iPSCs).

Naive Pluripotent Stem Cell Culture in Serum-Free Media

Naive pluripotent stem cells cultured in vitro using specialized media and inhibitors mimic "ground-state" cells from blastocysts.

Neural Stem Cell FAQs

Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.

Neuronal Mesenchymal Stem Cells

Protocol to differentiate human mesenchymal stem cells into neurons with extensive dendrites and axons in 3 days. Explore over 350 PromoCell products on Sigma.com.

ReNcell® Human Neural Progenitors: Renewable and Consistent Human Neural Stem Cell Lines that Differentiate into Functional Neurons

ReNcell neural progenitors are immortalized human neural stem cell lines that can differentiate into neurons, astrocytes sand oligodendrocytes.

Single Transfection of a Synthetic Polycistronic Self-replicative RNA Yields High Numbers of Transgene-free Human iPSCs

The Simplicon™ RNA Reprogramming Technology is a next generation reprogramming system that uses a single synthetic, polycistronic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells.

T Cell Expansion and Viability Using Stericup^®-filtered Media

Learn about the impact of filtration on hematopoietic cell culture media containing vital T cell factors and other media components.

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Introduction to Cell Transfection

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

Acid and Base Chart — Table of Acids & Bases

Acid and base chart lists the strength of acids and bases (strongest to weakest) in order. Simple to use laboratory reference chart for scientists, researchers and lab technicians.

Click Chemistry in Drug Discovery

Explore the principles and applications of click chemistry in drug discovery, highlighting efficient reactions that streamline the synthesis of bioactive compounds.

Crosslinkers Selection Guide

Choosing the right crosslinker for your biochemical or bioconjugation research can be challenging. Our selection guide can help you find the perfect match for your applications.

GlycoProfile™ Azido Sugars

GlycoProfile™ Azido Sugar portfolio offers peracetylated azido sugars for glycan structure modification via chemical or biosynthetic pathways.

Guanylation of Amines

Introduce guanidinyl group using guanylating reagents like bis(tert-butoxycarbonyl)thiopseudourea, polymer-bound, from substituted amines.

Polybrene^® Technical Bulletin

Polybrene® neutralizes red blood cell charge, a vital anti-heparin agent in various laboratory applications.

Baran Diversinates™

Rapidly diversify (hetero)aromatic scaffolds for chemical industry needs amid resource and time constraints, ensuring efficiency.

Catalysts for Carbonylation

Professor Geoffrey Coates and co-workers at Cornell University have reported the preparation and use of catalysts composed of an oxophilic Lewis acid and a cobalt tetracarbonyl anion for the ring expansive carbonylation of epoxides to b-lactones and b-lactones to succinic

Cationic Gold(I) Complexes Based on Bulky Phosphine Ligands: New Opportunity for Efficient Gold Catalysis

Chiral Disulfonimides for Asymmetric Counteranion-directed Catalysis (ACDC)

Asymmetric counteranion-directed catalysis (ACDC) has a number of valuable applications in enantioselective synthesis.

Cinchona Alkaloids

Asymmetric phase-transfer catalysis (PTC) has been recognized as a “green” alternative to many homogeneous synthetic organic transformations, and has found widespread application. Synthetically modified cinchona alkaloids are typical chiral organocatalysts used in asymmetric PTC.

DalPhos Ligands

DalPhos ligands enable Pd-catalyzed C-N and C-C bond formation with good functional group tolerance.

DIOP for Asymmetric Hydrogenation Catalysis

DIOP

Doyle Nickel Precatalyst Applications

Palladium catalysts in transition metal-catalyzed coupling reactions offer diversity, accessibility, and robustness over nickel catalysts.

DSM MonoPhos™ Ligands

DSM collaboration offers MonoPhos™ ligands for research, based on the BINOL platform by Feringa and co-workers.

G3 and G4 Buchwald Precatalysts

G3 and G4 Buchwald palladium precatalysts are the newest air, moisture, and thermally stable crossing-coupling complexes used in bond formation for their versatility and high reactivity.

Kwon Phosphines: P-Chiral Monodentate Phosphines from Hydroxyproline

Chiral phosphines have been the staple ligands for asymmetric transition metal catalysis and more recently operate as catalysts in organic phosphinocatalysis.

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