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Scalable Tangential Flow Filtration (TFF) for Downstream Processing of Viral Vectors for Gene Therapy and Vaccine Production

Our overview of scalable tangential flow filtration for viral vector production will provide key considerations for developing and optimizing your downstream process.

Water Determination in Liquefied Petroleum Gas using GC BID and Ionic Liquid Column Watercol™

Using Watercol™ GC columns in conjunction with GC-BID can provide a reliable and practical option for Water determination in petrochemical feedstocks.

재생 가능 전기 툴킷

재생 가능 전기 공급업체 툴킷은 공급업체가 재생 가능 전기의 기본 사항과 프로젝트를 실현하는 방법을 배울 수 있도록 도와줍니다.

Renewable Electricity Toolkit

The Renewable Electricity Supplier Toolkit helps suppliers learn renewable electricity basics and how to bring projects to life.

ChIC and CUT&RUN Data Analysis Tool Frequently Asked Questions (FAQs)

Chromatin Immunocleavage (ChIC) and CUT&RUN data analysis tool frequently asked questions (FAQs).

ChIC and CUT&RUN Data Analysis Tool Overview

Chromatin Immunocleavage (ChIC) and CUT&RUN data analysis tool for file management and downstream sequencing data analyses.

ChIC/CUT&RUN Kits

Chromatin Immunocleavage (ChIC) kits and CUT&RUN technology overview for improved chromatin isolation and downstream analyses.

Custom Next-Gen Sequencing Oligos

Regardless of next-generation sequencing (NGS) platform, universal and index adapter sequences are required for the proper assembly of sample fragments.

DNA Standards for Metagenomic Research

DNA standards enhance metagenomics research integrity, offering precise species study and mixed community standards.

Minimizing Dimerization of Next-Generation Sequencing Adapters

Turn to our Next-Gen Sequencing Oligos (NGSO) as a potential solution if you are experiencing high levels of adapter dimer formation with custom adapters sourced from other vendors.

siRNA Universal Neg. Controls

MISSION siRNA Negative Controls ensure mRNA knockdown efficiency baseline; tested in human, rat, and mouse cells, designed without gene homology.

SeqPlex™-I Transcriptome Amplification Kits

SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.

WTA2 Amplicon Modification for NGS

Transplex Whole Transcriptome Amplification (WTA2) precisely amplifies RNA maintaining transcript levels in test and reference samples.

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Water for Nucleic Acid Gel Electrophoresis

Learn how water impurities can affect DNA and RNA electrophoresis and blotting. High purity water purified using ultrafiltration is suitable for nucleic acid electrophoresis, Northern and Southern blotting.

Methods Used to Detect Proteins and Nucleic Acids Bound to Membranes

Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

KAPA Mouse Genotyping Kit FAQs

Review some of the most asked questions regarding the KAPA Mouse Genotyping Kits, which are ideally suited for the rapid extraction and amplification of DNA from mouse tail, ear and toe tissue.

DNA Component Explanation

Plasmid platform with interchangeable DNA components offers versatile research tools for genetic studies.

PCR Assay Optimization and Validation

PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.

Colony PCR

Colony PCR reagents and our colony PCR protocol using REDExtract-N-Amp™ PCR ReadyMix™ and JumpStart™ REDTaq® PCR ReadyMix™ reagents.

A highly efficient method to concentrate DNA for forensic STR genotyping using DNAstable®

Forensic laboratories routinely use STR genotyping for identity testing of biological samples. However, forensic samples often contain low copy numbers of target DNA, making it difficult to obtain complete STR profiles.

Custom cGMP Oligos

If you commercialize Molecular Diagnostics (MDx), develop Laboratory Developed Tests (LDTs), or conduct preclinical research on therapeutics, then your final kit, assay, or modality must have an extraordinary level of quality to comply with strict regulatory or study standards.

Oligonucleotide Quality Control & Quality Assurance

The foundation of Sigma's manufacturing process is a robust QMS, which drives compliance to ISO 9001:2008 certification.

Custom LAMP Primers in Partnership with New England Biolabs

Primers manufactured using proprietary purification adjustments to perform optimally in loop-mediated isothermal amplification assays.

DNA Oligonucleotide Synthesis

Learn about the steps in phosphoramidite solid-phase method of Oligonucleotide Synthesis.

Custom DNA/RNA Oligos & qPCR Probes Resources

Review resources for custom oligos, RNA oligos, and qPCR probes including online tools, case studies, and FAQs.

Dual-Labeled Probes in Digital PCR

Learn more about how digital PCR (dPCR) is used for absolute quantification and for analysis of minority sequences.

Extract-N-Amp™ Blood PCR Kit Protocol

The Extract-N-Amp Blood PCR Kits contain all the reagents needed to rapidly extract and amplify human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.

Extract-N-Amp Reagent: Effective PCR from FTA® Blood Cards

Extract-N-Amp simplifies DNA extraction and amplification, enabling PCR directly from FTA blood cards, reducing variability.

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