This product is packaged as a box of 8 tubes, 24 tubes, or 96 tubes.
UFC9030
Amicon® Ultra Centrifugal Filter, 30 kDa MWCO
sample volume 15 mL, regenerated cellulose membrane, MWCO 30 kDa
Amicon® Ultra Centrifugal Filter, 30 kDa MWCO
동의어(들):
15 mL sample volume, 30 kDa MWCO, Centrifugal concentrator
조직 및 계약 가격을 보려면 로그인를 클릭합니다.
제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
41104916
NACRES:
NB.24
eCl@ss:
36100101
Material:
polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing
Pore size:
30 kDa MWCO
Working volume:
≤15 mL , 15 mL
Sterility:
non-sterile
Packaging:
pkg of 24 pkg, pkg of 8 pkg, pkg of 96 pkg
다음으로 건너뛰기
material
polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg, pkg of 8 pkg, pkg of 96 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
15 mL sample volume
technique(s)
protein extraction: suitable, protein purification: suitable
L
12.1 cm (4.807 in.)
diam.
2.97 cm
filtration area
7.6 cm2
volume
15 mL
working volume
≤15 mL , 15 mL
pore size
30 kDa MWCO
Quality Level
greener alternative category
shipped in
ambient
1 of 4
이 품목 | UFC8030 | UFC203024 | UFC5030 |
|---|---|---|---|
| parameter 15 mL sample volume | parameter 4 mL sample volume | parameter 2 mL sample volume | parameter 0.5 mL sample volume |
| material polyethylene cap, polypropylene tube, regenerated cellulose support, polyethylene liner, styrene-butadiene housing, regenerated cellulose membrane | material polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing | material polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing | material polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing |
| manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra |
| pore size 30 kDa MWCO | pore size 30 kDa MWCO | pore size 30 kDa MWCO | pore size 30 kDa MWCO |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| product line Amicon® | product line Amicon® | product line Amicon® | product line Amicon® |
Application
- Ultrafiltration with 30,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS.
- Concentration of dilute or pre-purified proteins from column eluents.
- Desalting, buffer exchange and protein dialysis.
Features and Benefits
- Ultracel® regenerated cellulose membrane with 30 kDa NMWCO delivers retentate recovery of greater than 90%.
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes).
- Heat-sealed membrane minimizes downstream extractables.
- 100% integrity tested for reliable performance.
- Convenient sample monitoring with translucent housing and volume gradations.
- Direct pipettor sample access eliminates processing step to recover concentrate.
- High concentration factors of 80–100X.
General description
Amicon® Ultra 15 mL centrifugal devices with Ultracel® 30,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Ovalbumin (45 kDa) at 1 mg/ml initial concentration is retained at >95% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from 60% bio-circular material allocated using the ISCC mass balance approach. Click here for more information.
Physical form
Color Code: Clear with pink cap
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
문서
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
Filtration methods and membrane filter selection in nanoparticle purification and production.
나노입자 정제 및 생산에서 여과 방법 및 멤브레인 필터 선택.
프로토콜
면역글로불린을 위한 정제 프로토콜에는 단백질 A 또는 G 크로마토그래피 배지에 대한 친화성 결합, 비결합 단백질 세척, 낮은 pH 버퍼를 이용한 용리가 포함됩니다.
Purification protocols for immunoglobulins generally include affinity binding to Protein A or G chromatography media, washing unbound proteins, and eluting with a low pH buffer.
관련 콘텐츠
Purification methods for downstream analysis reduce sample complexity, remove interferences, and concentrate analytes for more accurate results.
다운스트림 분석을 위한 정제 방식은 시료의 복잡성을 줄이고 간섭을 제거하며 분석물을 농축하여 보다 정확한 결과를 얻을 수 있습니다.
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how many units are there in 1 pkg
1 답변-
도움이 되었습니까?
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How many individual columns come in one package. For example, if I wanted to purchase the 24package option, wil I get 24 columns or 24 packages of x number of columns?
1 답변-
A package of 24 would equate to 24 Ultra Centrifugal Filters.
도움이 되었습니까?
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Hi there, I need to extract exosome in the range of 30 to 150 nm. Which Ultra Centrifugal Filter is fit for my work? Thank you for your advice in advance.
1 답변-
We have performed studies of nanometer particles and all of our studies show that using a 100 kda molecular weight cutoff should be fine to trap particles greater than 10 nm.
도움이 되었습니까?
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Can this item be sterilised, and if so, what is the best method (e.g., autoclaving) to ensure that product function and integrity are maintained?
1 답변-
This item cannot be autoclaved. Sterilization with Ethylene Oxide is possible, but the method has not been validated.
도움이 되었습니까?
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Can the product be washed and re-used?
1 답변-
This product is intended for single use. Repeated usage may result in declining performance.
도움이 되었습니까?
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How many times can sample be added to the same filter unit? For example: If I have 100 mL cell free supernatant and want to add 12 mL at a time, centrifuge and collect retentate - followed by discarding the flow through and pouring the next 12 mL etc.
1 답변-
The same Amicon filter unit can be used for repeated spins with the same sample. However, repeated spins may lead to increased clogging, resulting in slower flow rates and longer spin times.
도움이 되었습니까?
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I noticed no volume change after centrifuging 10mL cell media at 14,000xg for 20minutes. Could this be due to unsuccessful concentration?
1 답변-
The lack of volume change suggests that the sample may contain a high amount of particulate matter, leading to rapid clogging and preventing effective concentration.
도움이 되었습니까?
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Hello! Our lab had to move to a different area of our building and we found some probably quite old boxes of Amicon Ultra 15s (Cat UFC903096, lot R8CN67048). Does this product have an expiration date/shelf life? If so, has this lot surpassed it?
1 답변-
This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one-year warranty from the date of shipment.
For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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