If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
P1379
TWEEN® 20
viscous liquid
동의어(들):
Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
크기 선택
| 팩 크기 | SKU | 재고 정보 | 가격 (VAT 별도) |
|---|---|---|---|
| 25 mL | 구입 가능 여부는 고객센터에 문의하십시오. | ₩41,514 | |
| 100 mL | 오늘 배송 가능재고 수량 조회Seoul Warehouse South Korea | ₩84,550 | |
| 250 mL | 오늘 배송 가능재고 수량 조회Seoul Warehouse South Korea | ₩94,903 | |
| 500 mL | 오늘 배송 가능재고 수량 조회Seoul Warehouse South Korea | ₩154,179 | |
| 1 L | 오늘 배송 가능재고 수량 조회Seoul Warehouse South Korea | ₩184,629 | |
| 4 L | 오늘 배송 가능재고 수량 조회Seoul Warehouse South Korea | ₩384,685 | |
| 6 x 1 L | 구입 가능 여부는 고객센터에 문의하십시오. | ₩562,412 |
제품정보 (DICE 배송 시 비용 별도)
description
non-ionic
Quality Level
assay
>40% (GC)
form
viscous liquid
mol wt
~1228
composition
lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)
concentration
≥40.0% (GC)
technique(s)
DNA extraction: suitable, RNA extraction: suitable, cell culture | mammalian: suitable, protein purification: suitable
impurities
≤3.0% water
refractive index
n20/D 1.468 (lit.)
CMC
0.06 mM (20-25°C)
acid number
≤2.2 mg/g
hydroxyl value
96‑108 mg/g
transition temp
cloud point 76 °C
density
1.095 g/mL at 25 °C (lit.)
HLB
16.7
suitability
complies for IR spectroscopy
application(s)
advanced drug delivery
SMILES string
CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
InChI
1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
InChI key
HMFKFHLTUCJZJO-UHFFFAOYSA-N
General description
Surfactants, possessing both hydrophilic and hydrophobic components, fall into two main categories: ionic, which ionize in water (anionic, cationic, or ampholytic), and nonionic, which do not ionize. The discussed product is a nonionic surfactant with diverse applications such as emulsification, dispersion, solubilization, and detergent use. Additionally, it can serve as a solubilizer for membrane proteins.
Application
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.
- washing buffer and blocking buffer in Western blotting
- blocking buffer in immunohistochemistry
- washing buffer in ELISA (enzyme linked immunosorbent assay)[1]
- reaction mixture in PCR (polymerase chain reaction)
Features and Benefits
- Highly versatile surfactant for your life science and biochemical research
- Excellent solubilising, dispersing and emulsifying agent
- Suitable for DNA & RNA extraction, mammalian cell culture, and protein purification
Packaging
Each kit contains 3 x 100ML samples, each sample from a uniquely manufactured lot.
Preparation Note
Other Notes
Legal Information
1 of 1
이 품목 | |||
|---|---|---|---|
| suitability complies for IR spectroscopy | suitability - | suitability - | suitability - |
| form viscous liquid | form viscous liquid | form viscous liquid | form viscous liquid |
| technique(s) DNA extraction: suitable, cell culture | mammalian: suitable, RNA extraction: suitable, protein purification: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable | technique(s) DNA extraction: suitable, RNA extraction: suitable, cell culture | mammalian: suitable, protein purification: suitable |
| assay >40% (GC) | assay >40% (GC) | assay - | assay - |
| CMC 0.06 mM (20-25°C) | CMC 60 mg/L | CMC 0.06 mM (20-25°C) | CMC - |
| density 1.095 g/mL at 25 °C (lit.) | density 1.095 g/mL at 25 °C ((lit.)), 1.095 g/mL at 25 °C (lit.) | density 1.095 g/mL at 25 °C (lit.), 1.1 g/cm3 at 25 °C (lit.) | density 1.11 g/mL at 20 °C, 1.095 g/mL at 25 °C (lit.) |
저장 등급
10 - Combustible liquids
flash_point_f
527.0 °F - Pensky-Martens closed cup
flash_point_c
275 °C - Pensky-Martens closed cup
ppe
Eyeshields, Gloves
프로토콜
This page shows how to solubilize membrane proteins with products from Cytiva.
문서
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
사용 가능한 형광 동소 교잡법(FISH) 절차, 시약, 장비.
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| P1379-100ML | 04061837602108 |
| P1379-1KT | 04061842429400 |
| P1379-4L | 04061834354611 |
| P1379-250ML | 04061838672940 |
| P1379-6X1L | 04061837602160 |
| P1379-1L | 04061837602115 |
| P1379-25ML | 04061837602139 |
| P1379-500ML | 04061837602153 |
| P1379-6X500ML | 04061837602177 |
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How can I determine the shelf life / expiration / retest date of this product?
1 답변-
도움이 되었습니까?
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What is the storage temperature of Tween 20?
1 답변-
This product may be stored at room temperature.
도움이 되었습니까?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 답변-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
도움이 되었습니까?
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What is the minimum dilution ratio of Tween20 in Exosome incubating buffer based on CMC level?
1 답변-
This product is not tested for any specific application. The critical micelle concentration of TWEEN 20 has been reported as 60 milligrams per liter. Please see the link below to review a publication that may be helpful:
https://open.clemson.edu/cgi/viewcontent.cgi?article=5274&context=all_theses도움이 되었습니까?
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Please provide the information on product container and material for P1379-500ML TWEEN® 20
1 답변-
This item is packaged in a 500 ML HDPE Bottle with a red PP cap w/ PTFE liner.
도움이 되었습니까?
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You state 0.06 mM as the CMC for this product. What is the stock concentration (in Molar) for this product? Alternatively what is the % to reach that 0.06 mM / CMC concentration? Thank you
1 답변-
The critical micelle concentration (CMC) reported on the product page is a value reported from the literature. This product is a neat or pure liquid. The base molecular weight of this material is 1,225 daltons and the density is 1.095 g/mL. However, this is based on the assumption of 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid. Please see the link below to review the general Tween 20 datasheet, note that the CMC value listed is 60 mg/L.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/128/958/p9416pis.pdf도움이 되었습니까?
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What is the grade/agency of Tween 20?
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This product is a research grade TWEEN 20.
도움이 되었습니까?
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What is the expiry date once opened?
1 답변-
Long-term stability studies have not been performed, however, providing that the product is stored and handled properly, the expected shelf life is not expected to change. Factors such as improper closure and cross-contamination would certainly impact the overall stability and shelf life.
도움이 되었습니까?
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The data sheet for my lot says this is 50% pure. Does that mean it is 50% tween 20?
1 답변-
The 50% is referring to the concentration of lauric acid which occurs in the highest concentration. Fatty acid constituents of this product are determined by transesterification to yield fatty acid methyl esters, which are identified by gas chromatography. Fatty acids that are typically present in the offered Tween products are not a quantitative measurement. These include myristic, palmitic, and stearic acid, for the most part. While the Tween is considered a 'pure' product, the molecular weight is calculated on the assumption of 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid.
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What is the Department of Transportation shipping information for this product?
1 답변-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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