Parafilm is not a sterile product. However, Parafilm can be sterilized using either radiation (UV) or chemical (H2O2) sterilization techniques. There is no protocol available. Please check the literature for additional information.
추천 제품
material
film (thermoplastic)
무균
non-sterile
포장
pack of 1 ea
제조업체/상표
Parafilm PM996
파라미터
-45-+50 °C temp. range
너비 × 길이
10 cm × 38 m
롤 크기
4 in. × 125 ft
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일반 설명
Protect the integrity of research applications with a tight moisture-barrier that holds a strong seal and doesn′t release from glassware, despite off-gassing
Reduce the risk of tampered data with a covering that is odorless, colorless and semi-transparent
Prevents moisture and volume loss due to material composition and tight seal
Flexible and self-sealing material allows it to cling around irregular shapes and surfaces
Stretches more than 200% of original length
Resistant up to 48 hours against many polar substances such as saline solutions, inorganic acids and alkaline solutions
Permeability
Oxygen: (ASTM 1927-98) 150 cc/m² d at 23°C and 50% RH
Carbon Dioxide: (Modulated IR Method) 1200 cc/m² d at 23°C and 0% RH
Water Vapor: (ASTM F1249-01)
Flat: 1 g/m² d at 38°C and 90% RH
Creased: 1 g/m² d at 38°C and 90% RH
Effects of Common Reagents
Potassium Permanganate
5%: No apparent effect except permanent dark brown coloration in 18 hours
0.1%: Same as 5% except slightly less color
Iodine solution (0.1N)
No effect except staining brown in 18 hours
Ethyl Alcohol (95%)
No apparent effect except some face whitening in 24 hours
No apparent effect in 24 hours:
Hydrochloric Acid conc. (12N) dil. (5N)
Sulphuric Acid conc. (36N) dil. (5N)
Nitric Acid conc. (16N) dil. (5N)
Sodium Hydroxide conc. (22%)
Ammonium Hydroxide conc. (28% NH<sub>3</sub>)
Salt (NaCl) solution (20%)
Isopropyl Alcohol (99%)
Not recommended for use with chlorinated, non-polar aliphatic and aromatic solvents
Film becomes soft and sticky at about 130° to 150°F (54° to 66°C)
법적 정보
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프로토콜
iPS 세포 해동, 증식, 냉동, 특성화를 포함한 인간 유도만능줄기세포(iPSC)를 위한 줄기세포 배양의 단계별 프로토콜.
Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.
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I'm designing an experiment which involves using Parafilm M as a coverslip for a bacterial culture. Therefore, I need it to sterilize it previously. Which methods are suitable for this process? Thank you!
1 답변-
도움이 되었습니까?
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Ich habe immer Parafilm M. (Bemis 12) genutzt. Nun habe ich die einzelnen Strips hier, und man kann das Trägerpapier nicht ablösen; zumindest weiß ich nicht wie. Gibt es da einen Trick oder klebt die Folie nach 3 Jahren einfach fest auf dem Träger?
1 답변-
Parafilm is generally very stable. the temperature range for continuous use is -45 to 50 deg. C. Storage at or excursion beyond this range may impact the physical properties making it difficult to use and possibly separate from the backing. Some tips to separate from the backing include rubbing between fingers, creasing or trying to tear an edge or corner, scraping or marking with a micro spatula or other firm edged item. Below see the general use video on Parafilm that uses the creasing method.
https://www.youtube.com/watch?v=-kw7rBJxH3o도움이 되었습니까?
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